Product | Characteristics | Selected applications |
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Dynabeads MyOne SILANE | - Monosized beads with an optimized silica-like surface chemistry
- Highly reproducible binding of both DNA and RNA
- High specific surface area
(approx. 20 m2/g) - Size: 1 micrometer, monodisperse
- Isoelectric point: pH 3.5
- Increased iron content compared to the traditional Dynabeads MyOne product range, and hence a better mobility to magnet
- Low sedimentation rate and favorable reaction kinetics
- Flexible starting volumes
| - Isolation of total nucleic acids (DNA/RNA)
- From biological samples (serum/plasma, human whole blood etc.)
- Well suited for automated applications
- Validation and customization (bead, buffer, protocol or format) available upon request.
- Adjusted to customer requirements on a custom OEM basis
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Dynabeads SILANE viral NA | - Contains Dynabeads MyOne SILANE (see above for characteristics), buffers and a protocol optimized for isolation of viral DNA/RNA
- High sensitivity for viral DNA and RNA, e.g. can detect HBV DNA in a 200 µl serum sample containing only 40 infectious units
| - Isolation of viral DNA/RNA from human serum/plasma samples
- Effective isolation of nucleic acids even from challenging viruses (e.g. HBV)
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Dynabeads SILANE genomic DNA | - Contains Dynabeads MyOne SILANE (see above for characteristics), buffers and a protocol optimized for isolation of genomic DNA
- High integrity and purity of isolated DNA
- Typically isolates 10 µg DNA from a 350 µl blood sample using 50 µl (2 mg) Dynabeads
- Linear range of DNA yield relative to white blood cell (WBC) counts
| - Isolation of genomic DNA from human whole blood
- From fresh/frozen blood samples, and with a wide range of anticoagulants
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Dynabeads Oligo(dT)25 | - Hydrophilic bead surface
- Size: 2.8 micrometer
- Iron content (ferrites): 14% (20%)
- Amount of coupled oligo-dT25 probe: 70 pmol/mg beads
- Capture of polyA mRNA: >2 µg/mg beads
| - Capture of polyA+ mRNA directly from crude starting samples
- Build a solid-phase cDNA library
- Specific capture of nucleic acid targets (using capture probes with oligo-dA tail)
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Dynabeads M-270 Streptavidin | - Hydrophilic bead surface
- Based on carboxylic acid beads
- Size: 2.8 micrometer, monodisperse
- Isoelectric point: pH 4.5
- Highly charged (-50 mV, at pH 7)
- Iron content (ferrites): 14% (20%)
- Low aggregation of beads in high salt solutions
- Free biotin binding: 650–1,350 pmoles/mg beads
- Binding biotinylated Ig: 5–10 µg/mg beads
| - Isolation of biotinylated molecules
- Sequence specific DNA/RNA capture in nucleic acid based diagnostics
- Preparation of single-stranded DNA.
- For use in protocols that require GTC lysis or high salt concentrations, or protocols not compatible with BSA.
- Immunodiagnostics with hydrophobic targets
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Dynabeads M-280 Streptavidin
| - Hydrophobic bead surface
- Based on tosylactivated beads
- Size: 2.8 micrometer, monodisperse
- BSA as blocking protein
- Isoelectric point: pH 5.0
- Low charge (-10 mV, at pH 7)
- Iron content (ferrites): 12% (17%)
- Free biotin binding: 650–900 pmoles/mg beads
- Binding biotinylated Ig: 5–10 µg/mg beads
| - Isolation of biotinylated molecules
- Sequence specific DNA/RNA capture in nucleic acid based diagnostics
- Purification of DNA/RNA binding proteins
- Protein purification, phage display and biopanning
- Immunodiagnostics
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Dynabeads MyOne Streptavidin C1 | - Hydrophilic bead surface
- Based on carboxylic acid beads (C1)
- Size: 1 micrometer, monodisperse
- No blocking proteins used
- Tween 20 in the buffer
- Isoelectric point: pH 5.2
- Medium charged (-35 mV, at pH 7)
- Iron content (ferrites): 26% (37%)
- Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads
- Very low aggregation
- Free biotin binding: >2,500 pmoles/mg beads
- Binding biotinylated Ig: 20–25 µg/mg beads
| - Isolation of biotinylated molecules
- Sequence specific DNA/RNA capture in nucleic acid based diagnostics
- Preparation of single-stranded DNA
- High throughput nucleic acid clean-up protocols
- Well suited for automated applications
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Dynabeads MyOne Carboxylic Acid | - Hydrophilic bead surface with carboxylic acid groups
- Size: 1 micrometer, monodisperse
- Iron content (ferrites): 26% (37%)
- Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads
- Very low aggregation
- Active chemical functionality: 0.6 mmol/g beads
- Coupling capacity: 20–25 µg proteins or 700 pmol oligonucleotides per mg beads
| - Immobilization of amino-modified oligonucleotides, antibodies or other ligands, for capture of specific DNA/RNA sequences, proteins or any other analyte
- Well suited for automated applications
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Dynabeads M-270 Carboxylic Acid | - Hydrophilic bead surface with carboxylic acid groups
- Size: 2.8 micrometer, monodisperse
- Iron content (ferrites): 26% (37%)
- Very low aggregation
- Active chemical functionality: 150 µmol/g beads
| - Immobilization of amino-modified oligonucleotides, antibodies or other ligands, for capture of specific DNA/RNA sequences, proteins or any other analyte
- Well suited for automated applications
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Dynabeads MyOne Tosylactivated
| - Hydrophobic bead surface
- Size: 1 micrometer, monodisperse
- Iron content (ferrites): 26% (37%)
- Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads
- Active chemical functionality: 0.04–0.06 mmol/g beads
| - Immobilization via primary amino- or sulfhydryl groups.
- Protein purification, phage display and biopanning
- Immunodiagnostics
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Dynabeads M-280 Tosylactivated
| - Hydrophobic bead surface with
p-toluene-sulfonyl (tosyl) groups - Size: 2.8 micrometer, monodisperse
- Iron content (ferrites): 12% (17%)
- Active chemical functionality: 0.1–0.2 mmol/g beads
| - Immobilization via primary amino- or sulfhydryl groups
- Protein purification, phage display and biopanning
- Immunodiagnostics
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