Reversed Phase HPLC Columns | Thermo Fisher Scientific

Overview

Selectivity at your fingertips

Solve your chromatography separation challenges with our reversed phase (RP) HPLC and UHPLC columns.

Reversed phase HPLC and UHPLC columns offer a wide variety of stationary phases for the effective separation of your analytes. Reversed phase stationary phases are available in a range of surface chemistries that vary in the length and structure of their hydrocarbon chains. Our reversed phase column portfolio includes options for C18, C8, C4, C30, polar-embedded phases, and aromatic group phases like phenyl, biphenyl, phenyl-hexyl, and PFP. These stationary phases also come in different sorbents such as porous silica, solid core silica, and polymeric resin. By switching between different stationary phases, it is possible to control how strongly your molecules of interest are retained within your column.

Popular Thermo Scientific reversed phase HPLC and UHPLC column brands include:

Hypersil GOLD fully porous premiere columns for excellent peak shape.
Accucore solid core premier columns for high-throughput applications.
Acclaim fully porous premier columns for complex samples.

All HPLC and UHPLC Columns

Reversed phase HPLC and UHPLC columns

Hypersil GOLD Columns

Fully porous, ultrapure silica particle columns
Built on 40 years of experience in column development and manufacturing
Excellent resolution, efficiency, and sensitivity for routine, high-quality separations

Accucore Columns

Solid core silica technology columns
UHPLC efficiency on any instrument
Rapid, high-resolution separation of complex samples and limited sample volumes
Increased sensitivity and peak capacity using standard HPLC

Acclaim Columns

Fully porous, highly retentive silica particle column
Ideal for complex mixtures that require higher resolution
Wide variety of column chemistries with various selectivities, including mixed-mode and HILIC

Learn more about our small molecule HPLC and UHPLC column brands ›

Hydrophobic column phases

C18 (U)HPLC Columns	C8 (U)HPLC Columns	C4 (U)HPLC Columns

Strong hydrophobic retention that will separate most small molecules The laboratory workhorse and most commonly used selectivity Great starting column for new applications	Medium hydrophobic retention Ideal for highly hydrophobic small molecule analyte Densely bonded phase for separating structurally different molecules such as planar and bulky analytes	Weak hydrophobic retention Excellent retention and resolution of proteins and other biomolecules Can also be used to separate very hydrophobic macromolecules

C18 reversed phase (U)HPLC columns

Column	Column Advantages	Column Compatibility
Hypersil GOLD C18 Selectivity	Endcapped, ultrapure, silica-based C18 column Exceptional peak shape and resolution for HPLC and LC/MS analyses using gradient methods	Uniguard Guard Cartridge Hold UHPLC Filters
Hypersil GOLD VANQUISH C18	Endcapped, ultrapure, silica-based C18 column Packed to withstand high-pressure applications for more viscous mobile phase compositions or higher flow rates	UHPLC Filters
Hypersil GOLD Peptide C18	Endcapped, ultrapure, silica based C18 column Includes a peptide-based QC test, providing ultimate confidence Unparalleled sequence coverage and higher peak capacity
Accucore C18	Endcapped, solid core, high-efficiency, high-throughput C18 column Achieve UHPLC efficiency on any instrument Rapid separation of complex samples Increased sensitivity and peak capacity	Uniguard Guard Cartridge Holders
Accucore XL C18	Endcapped, solid core, high-efficiency, high-throughput C18 column Larger particles for increasing throughput when using older methods or older systems Increased sensitivity and efficiency	Uniguard Guard Cartridge Holders
Accucore Vanquish C18+, 1.5 µm	1.5 µm solid core particles, packed to withstand high-pressure applications Rapid separation of complex samples Increased sensitivity and peak capacity	UHPLC Filters
Acclaim 120 C18	Endcapped, high-surface-area, silica-based C18 column Increased resolution and retention High retention and resolution of complex mixtures	Acclaim Guard Holder and Coupler
Acclaim VANQUISH C18	Endcapped, high-surface-area, silica-based C18 column Packed to withstand high-pressure applications for more viscous mobile phase compositions or higher flow rates Increased resolution and retention High retention and resolution of complex mixtures	UHPLC Filters

C8 reversed phase (U)HPLC columns

Column	Column Advantages	Column Compatibility
Hypersil GOLD C8	Endcapped, ultrapure, silica-based C8 column Excellent peak shape and resolution	Uniguard Guard Cartridge Holders UHPLC Filters
Acclaim 120 C8	Endcapped, high-surface-area, silica-based C8 column Increased resolution and retention High retention and resolution of complex mixtures	Acclaim Guard Holder and Coupler
Accucore C8	Endcapped, solid core, high-efficiency, high-throughput C8 column Increased sensitivity and peak capacity	Uniguard Guard Cartridge Holders
Accucore XL C8	Endcapped, solid core, high-efficiency, high-throughput C18 column Larger particles for increasing throughput when using older methods or older systems Increased sensitivity and peak capacity	Uniguard Guard Cartridge Holders

C4 reversed phase (U)HPLC columns

Column	Column Advantages	Column Compatibility
Hypersil GOLD C4	Endcapped, ultrapure, silica-based C4 column Ideal for large peptides due to silica purity Excellent peak shape and resolution	Uniguard Guard Cartridge Holders UHPLC Filters
Accucore 150-C4	Endcapped, solid core, high-efficiency, high-throughput C4 column Large pore size provides better peak shapes when analyzing larger peptides Increased sensitivity and peak capacity	Uniguard Guard Cartridge Holders

Polar-modified C18 column phases

Polar-Endcapped C18 (U)HPLC Columns	Amide-Embedded C18 (U)HPLC Columns	Sulfonamide-Embedded C18 (U)HPLC Columns

Endcapped C18 phase with secondary polar selectivity Excellent for applications with mixed polar and non-polar analytes Can withstand 100% water mobile phase	Polar-embedded C18 phase with amide-embedded functional group Provides great retention and excellent peak shapes for polar bases Can withstand 100% water mobile phase	Polar-embedded C18 phase with sulfonamide-embedded functional group Provides great retention and excellent peak shapes for polar bases and polar acids Can withstand 100% water mobile phase

Polar-endcapped C18 reversed phase (U)HPLC columns

Column	Column Advantages	Column Compatibility
Hypersil GOLD aQ C18 Polar-Endcapped	Polar-endcapped, ultrapure, silica-based C18 column Exceptional peak shape and resolution for HPLC and LC/MS analyses using gradient methods	Uniguard Guard Cartridge Holders UHPLC Filters
Hypersil GOLD VANQUISH aQ	Polar-endcapped, ultrapure, silica-based C18 column Packed to withstand high-pressure applications for more viscous mobile phase compositions or higher flow rates	UHPLC Filters
Accucore aQ C18 Polar-Endcapped	Polar-endcapped, solid core, high-efficiency, high-throughput C18 column Achieve UHPLC efficiency on any instrument Rapid separation of complex samples Increased sensitivity and peak capacity	Uniguard Guard Cartridge Holders

Amide-embedded C18 reversed phase (U)HPLC columns

Column	Column Advantages	Column Compatibility
Accucore Polar Premium	Endcapped, solid core, high-efficiency, high-throughput, polar-embedded C18 column Achieve UHPLC efficiency on any instrument Rapid separation of complex samples Increased sensitivity and peak capacity	Uniguard Guard Cartridge Holders
Acclaim Polar Advantage II (PA 2)	Endcapped, high-surface-area, silica-based, polar-embedded C18 column Increased resolution and retention High retention and resolution of complex mixtures	Acclaim Guard Holder and Coupler
Acclaim VANQUISH Polar Advantage II (PA 2)	Endcapped, high-surface-area, silica-based, polar-embedded C18 column Packed to withstand high-pressure applications for more viscous mobile phase compositions or higher flow rates Increased resolution and retention High retention and resolution of complex mixtures	UHPLC Filters

Sulfonamide-embedded C18 reversed phase (U)HPLC columns

Column	Column Advantage	Column Compatibility
Acclaim Polar Advantage (PA)	Endcapped, high-surface-area, silica-based, polar-embedded C18 column Increased resolution and retention High retention and resolution of complex mixtures	Acclaim Guard Holder and Coupler

Specialty hydrophobic column phases

Accucore RP-MS (U)HPLC Columns	C30 (U)HPLC Columns

Densely-bonded proprietary hydrophobic selectivity phase that allows for geometrical separation as well as hydrophobic retention C18-like selectivity Excellent for MS applications where speed and sensitivity are of the essence	Large, bulky phase that combines hydrophobic selectivity and geometrical separations C30 ligands bend over themselves when bonded onto the surface of silica packing material, forming pockets that create geometrical interactions with analytes C18-like selectivity Ideal for the separation of longer-chained isomers such as fatty acids and vitamin K

Accucore RP-MS reversed phase (U)HPLC columns

Specialty hydrophobic column phases

Column	Column Advantage	Column Compatibility
Accucore RP-MS	Endcapped, solid core, high-efficiency, high-throughput column Achieve UHPLC efficiency on any instrument Rapid separation of complex samples Increased sensitivity and peak capacity	Uniguard Guard Cartridge Holders

C30 reversed phase (U)HPLC columns

Column	Column Advantage	Column Compatibility
Accucore C30	Endcapped, solid core, high-efficiency, high-throughput C30 column Achieve UHPLC efficiency on any instrument Rapid separation of complex samples Increased sensitivity and peak capacity	Uniguard Guard Cartridge Holders
Acclaim C30	Endcapped, high-surface area, silica-based, polar-embedded C30 column High retention and resolution of complex mixtures	Acclaim Guard Holder and Coupler

Phenyl-based column phases

Phenyl and Phenyl-Hexyl (U)HPLC Columns	Biphenyl (U)HPLC Columns	PFP (Pentafluorophenyl) (U)HPLC Columns

Moderately hydrophobic traditional phenyl phases that separate samples via aromatic interactions such as pi-pi stacking and dipol-dipol interactions Orthogonal selectivity to traditional hydrophobic phases for challenging separations that require aromatic interactions Excellent for separations of aromatic and moderately polar analytes	Strongly hydrophobic phenyl phase that separates samples via aromatic interactions such as pi-pi stacking and dipol-dipol interactions Orthogonal selectivity to traditional hydrophobic phases for challenging separations that require aromatic and strong hydrophobic interactions Ideal for small molecule pharmaceutical and forensic applications such as the analysis of drugs of abuse	Strong aromatic interaction phase that separates analytes through more permanent pi-pi stacking Orthogonal selectivity to traditional hydrophobic phases for challenging separations that require strong aromatic interactions Excellent for aromatic isomers as well as polar and halogenated aromatic compounds

Phenyl and phenyl-hexyl reversed phase (U)HPLC columns

Column	Column Advantages	Column Compatibility
Hypersil GOLD Phenyl	Endcapped, ultrapure, silica-based phenyl column Exceptional peak shape and resolution for HPLC and LC/MS analyses using gradient methods	Uniguard Guard Cartridge Holders
Accucore Phenyl-Hexyl	Endcapped, solid core, high-efficiency, high-throughput phenyl-hexyl column Achieve UHPLC efficiency on any instrument Rapid separation of complex samples Increased sensitivity and peak capacity	Uniguard Guard Cartridge Holders

Biphenyl reversed phase (U)HPLC columns

Column	Column Advantages	Column Compatibility
Accucore Biphenyl	Endcapped, solid core, high-efficiency, high-throughput biphenyl column Achieve UHPLC efficiency on any instrument Rapid separation of complex samples Increased sensitivity and peak capacity	Uniguard Guard Cartridge Holders

PFP reversed phase (U)HPLC columns

Column	Column Advantages	Column Compatibility
Hypersil GOLD PFP	Endcapped, ultrapure, silica-based PFP column Exceptional peak shape and resolution for HPLC and LC/MS analyses using gradient methods	Uniguard Guard Cartridge Holders
Hypersil GOLD VANQUISH PFP	Endcapped, ultrapure, silica-based PFP column Packed to withstand high-pressure applications for more viscous mobile phase compositions or higher flow rates	Uniguard Guard Cartridge Holders
Accucore PFP	Endcapped, solid core, high-efficiency, high-throughput PFP column Achieve UHPLC efficiency on any instrument Rapid separation of complex samples Increased sensitivity and peak capacity	Uniguard Guard Cartridge Holders