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Low-flow chromatography is ideal when detailed sample information is required from small sample volumes, such as in omics research. Compared with analytical flow (>100 µL/min), low-flow LC-MS (<100 µL/min) typically provides a large increase in sensitivity. Low-flow columns—also known as nano, capillary, or micro columns—have a smaller diameter compared to analytical columns.
Thermo Scientific nano-, capillary-, and micro-flow columns offer excellent sensitivity and resolution in easy-to-use formats:
Learn more about the power of Thermo Scientific low-flow HPLC columns and discover column designs that ensure near zero-dead volume connections to give you confidence in your data quality.
Our patented technology overcomes the physical limitations of packed bed LC columns. It reduces peak dispersion which leads to better separation. Furthermore, this unique technology increase data reproducibility and decrease costs as these columns are highly robust and reliable.
Improve your chromatography and analyze hundreds of samples per day, day after day, and do it without sacrificing performance and reproducibility.
Low-flow (U)HPLC column formats | ||
EASY-Spray (U)HPLC Columns | Single and Double nanoViper UHPLC Columns | µPAC Neo HPLC Columns |
Choose an EASY-Spray column when: | What makes an EASY-Spray column special? |
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| Unique design provides uncompromised performance in an easy-to-use format for nano and capillary LC-MS analysis.
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The Thermo Scientific EASY-Spray PepMap Neo UHPLC columns are perfect for bottom-up proteomics. Packed at higher pressure and rated to 1500 bar, they provide consistent column-to-column performance, long column lifetime, and excellent efficiency. These benefits are true at any pressure.
Catalog number | Product line | Particle size (µm) | Column ID (µm) | Column length (mm) | Order |
---|---|---|---|---|---|
ES75150PN | PepMap Neo columns | 2 | 75 | 150 | Order now › |
ES75500PN | PepMap Neo columns | 2 | 75 | 500 | Order now › |
ES75750PN | PepMap Neo columns | 2 | 75 | 750 | Order now › |
Also see: EASY-Spray PepMap HPLC columns
The Thermo Scientific MAbPac RP capillary column is best suited for the characterization of intact proteins in top-down proteomics, clinical and anti-doping applications where sample amount is limited, or sensitivity is crucial.
Catalog number | Product line | Particle size (µm) | Column length (mm) | Column ID (µm) | Order |
---|---|---|---|---|---|
ES907 | MAbPac RP column | 4 | 150 | 150 | Order now › |
For the best performance from your EASY-Spray column, consider investing in the following accessories.
Catalog number | Product line | Particle size (µm) | Column ID (µm) | Media bed length (mm) | Trap length (mm) | Union type | Order |
---|---|---|---|---|---|---|---|
174500 | PepMap Neo Trap Cartridge 3PK | 5 | 300 | 5 | N/A | N/A | Order now › |
164199 | PepMap nanotrap 500 bar 2PK | 5 | 100 | 20 | 150 | Nut/sleeve | Order now › |
164750 | PepMap nanotrap 500 bar 2PK | 5 | 100 | 20 | 150 | Double nanoViper | Order now › |
164535 | PepMap nanotrap 500 bar 2PK | 3 | 75 | 20 | 150 | Double nanoViper | Order now › |
164946 | PepMap nanotrap 1200 bar 2PK | 3 | 75 | 20 | 70 | Double nanoViper | Order now › |
164213 | PepMap nanotrap 500 bar 2PK | 5 | 200 | 20 | 150 | Nut/sleeve | Order now › |
Catalog number | Product line | For use with | Order |
---|---|---|---|
174501 | PEEK with nanoViper fittings 30 µm X100 mm 2PK 1500 bar | Low-flow PepMap columns | Order now › |
174502 | Trap holder + nanoViper fittings kit 1500 bar | Low-flow PepMap columns | Order now › |
Choose these columns when: | What makes these columns special? |
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| These stand-alone nano-, capillary, and micro-flow columns are:
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Use these columns for peptide bottom-up proteomics studies. They are packed at higher pressure and rated a 1500 bar and provide consistent column-to-column performance, long column lifetime, and excellent efficiency. These benefits are true at any pressure.
Catalog number | Product line | Particle size (µm) | Column ID (µm) | Column length (mm) | Order |
---|---|---|---|---|---|
DNV75150PN | PepMap Neo column | 2 | 75 | 150 | Order now › |
DNV75500PN | PepMap Neo column | 2 | 75 | 500 | Order now › |
DNV75750PN | PepMap Neo column | 2 | 75 | 750 | Order now › |
DNV150150PN | PepMap Neo column | 2 | 150 | 150 | Order now › |
SNV75750PN | PepMap Neo column | 2 | 75 | 750 | Order now › |
SNV150150PN | PepMap Neo column | 2 | 150 | 150 | Order now › |
Also available: Single nanoViper PepMap C18 HPLC columns
The Thermo Scientific MAbPac RP capillary column is best suited for the characterization of intact proteins in top-down proteomics, clinical, and anti-doping applications where sample amount is limited, or sensitivity is crucial.
Catalog number | Product line | Particle size (µm) | Column ID (µm) | Column length (mm) | Order |
---|---|---|---|---|---|
164947 | MAbPac RP column | 4 | 150 | 150 | Order now › |
Catalog number | Product line | Particle size (µm) | Column ID (µm) | Column length (mm) | Order |
---|---|---|---|---|---|
16726-157569 | Amide HILIC column | 2.6 | 75 | 150 | Order now › |
These emitters, nanoViper tubing kits, and unions offer easy connection from your LC system to an EASY-Spray source.
Catalog number | Product line | For use with | Order |
---|---|---|---|
6040.2304 | Two Viper unions | Single and Double nanoViper columns | Order now › |
6041.5260 | nanoViper tubing 20 µm x 550 mm | Single and Double nanoViper columns | Order now › |
ES993 | Emitter: 10 µm I.D. | Single and Double nanoViper columns | Order now › |
ES994 | Emitter: 15 µm I.D | Single and Double nanoViper columns | Order now › |
For the best performance from your double nanoViper column consider investing in the following accessories.
Catalog number | Product line | Particle size (µm) | Column ID (µm) | Media bed length (mm) | Trap length (mm) | Union type | Order |
---|---|---|---|---|---|---|---|
174500 | PepMap Neo Trap Cartridge 3PK | 5 | 300 | 5 | N/A | N/A | Order now › |
164199 | PepMap nanotrap 500 bar 2PK | 5 | 100 | 20 | 150 | Nut/sleeve | Order now › |
164750 | PepMap nanotrap 500 bar 2PK | 5 | 100 | 20 | 150 | Double nanoViper | Order now › |
164535 | PepMap nanotrap 500 bar 2PK | 3 | 75 | 20 | 150 | Double nanoViper | Order now › |
164946 | PepMap nanotrap 1200 bar 2PK | 3 | 75 | 20 | 70 | Double nanoViper | Order now › |
164213 | PepMap nanotrap 500 bar 2PK | 5 | 200 | 20 | 150 | Nut/sleeve | Order now › |
Catalog number | Product line | For use with | Order |
---|---|---|---|
174501 | PEEK with nanoViper fittings 30 µm X100 mm 2PK 1500 bar | Low-flow PepMap Neo columns | Order now › |
174502 | Trap holder + nanoViper fittings kit 1500 bar | Low-flow PepMap Neo columns | Order now › |
Choose a μPAC Neo HPLC column when: | What makes µPAC Neo HPLC columns special? |
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| The unique separation path provides:
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These HPLC columns are ideally suited for bottom-up proteomics where resolution is critical to the success of the analysis. Our µPAC Neo columns provide excellent column-to-column reproducibility and peak capacity at a wide range of flow rates and sample amounts.
Catalog number | Product line | Column length (cm) | Flow rate range (nL/min) | Sample loading | Gradient time | Order |
---|---|---|---|---|---|---|
COL-NANO050NEOB | µPAC Neo HPLC Column | 50 | 100-750 | 10-500 ng | 15 – 60 min | Order now › |
COL-NANO110NEOB | µPAC Neo HPLC Column | 110 | 100-750 | 500-2000 ng | 90-150 min | Order now › |
COL-LOLO050NEOB | Low-Load µPAC Neo HPLC Column | 50 | 100-750 | 0.1-1 ng | 15 – 60 min | Order now › |
COL-CAPHTNEOB | High-Throughput µPAC Neo HPLC Column | 5.5 | 100-2500 | 10-500 ng | < 15 min | Order now › |
Catalog number | Product line | For use with | Flow rate range (μL/min) | Sample loading | Order |
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COL-TRPLOLONEOB2 (Duo-Pack) | μPAC Neo low-load trapping column | μPAC Neo low-load HPLC column | 100-750 | < 10 ng | Order now › |
Catalog number | Product line | For use with | Order |
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6040.2304 | Union for Viper and nanoViper Tubing | Double nanoViper and μPAC Neo Columns | Order now › |
6041.5260 | nanoViper tubing 20 μm x 550 mm | Double nanoViper and μPAC Neo Columns | Order now › |
ES993 | Nano EASY-Spray emitter, 10 μm I.D. | Double nanoViper and μPAC Neo Columns | Order now › |
These HPLC columns are ideally suited for bottom-up proteomics where resolution is critical to the success of the analysis. Our µPAC HPLC columns provide excellent column-to-column reproducibility and peak capacity at a wide range of flow rates.
Catalog number | Product line | Pillar diameter (µm) | Interpillar distance (µm) | Column length (cm) | Flow rate range (µL/min) | Order |
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COL-nano050G1B | µPAC pillar array | 5 | 2.5 | 50 | 0.1 – 2.0 | Order now › |
COL-nano200G1B | µPAC pillar array | 5 | 2.5 | 200 | 0.1 - 1.0 | Order now › |
Catalog number | Product line | Pillar diameter (µm) | Interpillar distance (µm) | Column length (cm) | Order |
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COL-trpnano16G1B2 (Duo-Pack) | µPAC pillar array | 5 | 2.5 | 1 | Order now › |
Catalog number | Product line | For use with | Order |
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EMI-flexionB | μPAC Flex iON Connect ESI-MS: Interface to connect μPAC nanoLC columns to a Nanospray Flex Series ion source | μPAC nano columns | Order now › |
EMI-easysprayB | μPAC Compatible EASY-Spray Emitter: Pre-assembled EASY-Spray emitter, grounding union, and transfer line to connect μPAC nanoLC columns to an EASY-Spray source | μPAC nano columns | Order now › |
The all-in-one nano-, capillary-, and micro-flow LC system for high sensitivity LCMS workflows.
Webinar: Workflows for robust and sensitive separation of mAbs, intact proteins at subunit and peptide level by LC-MS
Download this guide and learn more about the portfolio of Thermo Scientific consumables for LC-MS proteomics research and the value they can bring to your lab.
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