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Water Microbiology Testing: Test for Safe Water
Microbiology products for the isolation, identification and enumeration of waterborne microorganisms to meet your testing needs.
Our range of products for water testing includes dehydrated and prepared media, membrane filters, qPCR assays for Legionella, quality control organisms, as well as the equipment you need to deliver reliable results.
- Safe: media formulations that are compliant with ISO Standards for the testing of water
- Conform to ISO standard: quality control testing methods for culture media are accredited in accordance with ISO 17025:2017
- Proven and certified: all Certificates of Analysis (CoAs) for culture media confirm testing in accordance with the ISO 11133:2014 standard, including membrane filter testing
- Support: our team of microbiology experts is at hand to help with your technical queries
The convenience of a prepared, sterile solution when preparing water samples for Legionella testing
Conveniently prepare water samples when isolating and enumerating Legionella species according to EN ISO 11731 water quality testing specifications using Thermo Scientific Legionella Acid Buffer Solution. The convenient high-quality, transparent PET bottle has a leak-proof screw top made of PET and is lighter and more shatter-resistant than glass, making it better for handling in the laboratory as well as shipping.
Legionella anisa QC testing strain available
To support your laboratory’s water testing processes, we have added Thermo Scientific Culti-Loops Quality Control Legionella anisa NCTC 11974 (WDCM 00106) to our quality control testing organism range. Culti-Loops Quality Control organisms enable quick and safe preparation of cultures for QC testing with ready-to-use, disposable inoculation loops containing gel-stabilised micro-organisms.
Legionella anisa is the second most often isolated Legionella species from water samples, after Legionella pneumophila.
Detection of microorganisms: ISO 6222:1999
Water sample |
Poured plate method with Yeast Extract Agar |
68±4 h at 20±2 °C and 44±4 h at 36±2 °C |
Count all colonies formed |
Detection of Escherichia coli / Coliform bacteria: ISO 9308-1:2014
Water sample |
Membrane filtration, using filter on surface of CCA Agar |
36±2 ºC 21±3 h |
| |
Count all rose colonies as coliforms | Count all dark blue to purple colonies as E.coli |
| |
Test oxidase with freshly prepared reagentNegative: No blue colonies | Confirmed E.coli |
| |
> Count all Oxidase-negative bacteria as coliforms> Count all Oxidase-positive bacteria as non-coliforms |
| Method | Media | Product | Format | Product code |
|---|---|---|---|---|
| Membrane filtration | CCA Agar | Thermo Scientific™ Chromogenic Coliform Agar | 500 g Dehydrated culture media | CM1205B |
| 10 x 90 mm plates Prepared media | PO5318A | |||
| 10 x 55 mm plates Prepared media | PO5428J | |||
| Oxidase test | Tryptone Soya Agar (TSA) | CASO AGEN (TSA) | 500 g / 5 kg Dehydrated culture media | CM0131B/T |
| 10 x 90 mm plates Prepared media | PO5012A | |||
| Oxidase Reagent | Thermo Scientific™ BactiDrop™ Oxidase Reagent | 50 vials Dehydrated culture media | R21540 | |
| 10 x 90 mm plates Prepared media | PO5321A | |||
| Thermo Scientific™ Microbact™ Oxidase Strips | 50 strips Dehydrated culture media | MB0266A |
Detection of Enterococci: ISO 7899-2:2000
Water sample |
Membrane filtration, using filter on surface of Slanetz and Bartley Agar |
36±2 °C 44±4 h |
Count all convex red, brown or rose colonies |
Put filter onto pre-warmed 44°CBile Aesculine Azide Agar |
44±0.5°C 2 h |
Enumerate all brown colonies with brown halos as intestinal Enterococci |
| Method | Media | Product | Format | Product code |
|---|---|---|---|---|
| Membrane filtration | Slanetz Bartley Agar | Slanetz Bartley Agar | 500 g Dehydrated culture media | CM0377B |
| 10 x 90 mm plates Prepared media | PO5018A | |||
10 x 55 mm plates Prepared media | PO5410J* | |||
10 x 55 mm plates Prepared media | PO5423J* | |||
| Confirmation | Bile Aesculine Azide Agar | Enterococcus Selective Medium (Bile Aesculine Azide Agar)1 | 10 x 90 mm plates Prepared media | PO5062A |
| or Kanamycin Aesculin Azide Agar Base2 | 500g Dehydrated culture media | CM0591B | ||
| 10 x 90 mm plates Prepared media | PO5059A |
* Minimum order quantity required.
1. This medium is slightly different to the medium formulation according to ISO 7899-2:2000. The medium has additional sodium citrate and 20 g/l ox bile instead of 10 g/l and 0.55 g/l sodium azide instead of 0.15 g/l.
2. This medium is slightly different to the medium formulation according to ISO 7899-2:2000. It contains sodium citrate instead of ox bile and Kanamycin.
Detection of Pseudomonas aeruginosa: ISO 16266:2006
Water sample |
Membrane filtration, using filter on surface of Pseudomonas CN Agar |
36±2 °C 44±4 h* |
| | |
Inspection under UV light(360±20 nm) | Inspection under natural light | Inspection under natural light |
| | |
Fluorescence colonies: presumptive Pseudomonas aeruginosa | Blue green (Pyocyanine forming) colonies: confirmed Pseudomonas aeruginosa | Red brown colonies: presumptive Pseudomonas aeruginosa |
| | |
Subculture to Nutrient Agar | Subculture to Nutrient Agar | |
| | |
36±2°C 22±2 h | 36±2°C 22±2 h | |
| | |
Add Nessler´s Reagent ammonia development (red-yellow precipitation) | Oxidase test: oxidase positive | |
| | |
Confirmed Pseudomonas aeruginosa | Acetamide Solution | King´s B Medium |
| | |
36±2°C 22±2 h | 36±2°C up to 5 days | |
| | |
Add Nessler´s Reagent ammonia development (red-yellow precipitation) | Fluorescence under UV light | |
| | |
Confirmed Pseudomonas aeruginosa | ||
* Minimum order quantity required.
| Method | Media | Product | Format | Product code |
|---|---|---|---|---|
| Membrane filtration | Pseudomonas Selective Agar / CN Agar | Pseudomonas Centrimide Selective Agar | 10 x 90 mm plates Prepared media | PO5076A |
| 10 x 55 mm plates Prepared media | PO5413J* | |||
| Pseudomonas Agar Base | 500 g Dehydrated culture media | CM0559B | ||
| 10 tubes Dehydrated culture media | SR0102E | |||
| Confirmation | ||||
| Fluorescence | King´s B Media | |||
| Hydrolysis of acetamide to ammonia | Acetamide Solution Nessler Reagent | |||
| Oxidase Test | Nutrient Agar | Nutrient Agar | 500 g Dehydrated culture media | CM0003B |
| Oxidase Reagent | BactiDrop Oxidase Reagent | 50 vials Dehydrated culture media | R21540 | |
| Microbact Oxidase Strips | 50 strips Dehydrated culture media | MB0266A | ||
* Minimum order quantity required.
Detection of Clostridium perfringens (including spores): ISO 14189:2013
Membrane filtration of 100 mL onto TSC-Agar* (if necessary, dilute sample) |
44±1°C, anaerobic 21±3 h |
Enumerate the presumptive colonies which show black or grey to yellow brown staining within 30 minutes of opening |
Count all black colonies as Clostridium perfringens |
| |
1-10 black or grey to yellow-brownish colonies | >10 black or grey to yellow-brownish colonies |
| |
Subculture all presumptive colonies onto blood agar plates | Subculture 10 different presumptive colonies onto blood agar plates |
| |
36±2°C, anaerobic 21±3 h | 36±2°C, anaerobic 21±3 h |
| |
Put colonies that grew under anaerobic conditions onto filter paper and add 2-3 drops of phosphateacid. All colonies that turn purple within 3-4 minutes are confirmed positive. | |
*ATTENTION: Alternatively, a thin layer (approximately 5 mL to 10 mL) of molten TSC Agar (TV5204G TSC Agar Base), as an overlay on the filter can be used. Allow to solidify before anaerobic incubation. This method may enhance the blackening of the colonies. Medium without cycloserine; e.g. TV5204G TSC Agar Base, 20 mL.
Melt TSC Agar in a water bath and put melted agar onto filter on the plate:
Melt agar in a water bath at 95°C for approx. 10-15 minutes |
Cool medium to 45°C |
Shake tube carefully and pour a thin layer of molten agar |
| Method | Media | Product | Format | Product code |
|---|---|---|---|---|
| Membrane filtration | TSC Agar | TSC Selective Agar | 10 x 90 mm plates Prepared media | PO5315A |
| TSC Agar Base + | 500 g Dehydrated culture media | CM0587B | ||
| 50 tubes Prepared media | TV5204G | |||
| TSC Selective Supplement | 10 tubes Dehydrated culture media | SR0088E | ||
| 10 x 100 mL bottles Prepared media | BO0634M | |||
| 10 tubes Prepared media | SR0088E | |||
| Anaerobic atmosphere | Thermo Scientific™ AnaroGen™ System | 10 sachets | AN0025A | |
| Thermo Scientific™ AnaroJar™ 2,5 L Anaerobic System | 1 unit | AG0025A | ||
| Thermo Scientific™ AnaeroBox™ Rectangular Anaerobic System | 1 unit | AB0025A |
Detection of Legionella: ISO 11731:2017
Sample preparation:
Heat treated: Put sample into water bath for 30+/-2 minutes at 50 °C
Acid treated: 1 part sample to 9 parts of acid buffer. Mix well and leave it for 5+/- 0.5 minutes.
Acid treated filter: Put 30 mL of acid solution onto the membrane filter. Leave it for 5 +/- 0.5 minutes and remove the solution by filtration. Wash with 20 mL of either sterile Aqua dest., Pages Saline, Ringer Solution or PBS.
Samples with high concentration of Legionella species and low concentration of interfering organisms2 | Samples with low concentration of Legionella species and low concentration of interfering organisms2 | ||
| | | |
0.1-0.5 mL of the sample onto BCYE | 0.1-0.5 of the sample onto BCYE with AB | Membrane filtration of 100 mL sample1.Place one untreated membrane filter onto BCYE Agar; Place membrane filter with acid solution on one or more selective media: BCYE Agar with AB or GVPC Agar or MWY Agar | 0.1-0.5 mL of the sample; each untreated, heat treated and acid treated from the membrane filtration with washing procedure3 onto BCYE Agar and on one or more selective media: BCYE Agar with AB or GVPC Agar or MWY Agar |
| | | |
36 ± 2 °C, humid atmosphere, 7-10 days | |||
| |||
Subculture at least 3 typical Legionella colonies from each plate and inoculate on BCYE Agar without Cystein and BCYE Agar | |||
| |||
36 ± 2 °C, humid atmosphere, 2-5 days | |||
| |||
Colonies that grow on BCYE but not on BCYE without cystein are considered to be Legionella3 | |||
- 10 – 1000 mL sample volume according to ISO 11731-2.
- The choice of the method for the enumeration of Legionella species depends on the origin/characteristics of the sample and the reason for sampling or investigation. A decision matrix can be found in the ISO 11731:2017, Annex J.
- Further information, please see ISO 11731:2017.
| Method | Media | Product | Format | Product code |
|---|---|---|---|---|
| Sample preparation | Acid Buffer | Legionella Acid Buffer Solution | 6 x 1 L bottles Prepared media | GFB01 |
| Direct and membrane filtration | Legionella GVPC Agar | Legionella GVPC Selective Agar | 10 x 90 mm plates Prepared media | PO5074A |
| 10 x 90 mm plates Prepared media | PO0245A | |||
| Legionella CYE Agar-Base + | 500 g Dehydrated culture media | CM1203B | ||
| Legionella BCYE Supplement + | 10 tubes (500 mL) Dehydrated culture media | SR0251C | ||
| Legionella GVPC Selective Supplement | 10 tubes (500 mL) Dehydrated culture media | SR0252E | ||
| Legionella GVPC Agar with antibiotics | Legionella BCYE Agar with antibiotics | 10 x 90 mm plates Prepared media | PO5325A | |
| Legionella BCYE Agar | Legionella BCYE Agar | 10 x 90 mm plates Prepared media | PO5072A | |
| Legionella CYE Agar Base + | 500 g Dehydrated culture media | CM1203B | ||
| Legionella BCYE Supplement | 10 tubes (500 mL) Dehydrated culture media | SR0251C | ||
| Legionella MWY Agar | Legionella MWY Agar | 10 x 90 mm plates Prepared media | PO5071A | |
| Legionella CYE Agar Base + | 500 g Dehydrated culture media | CM1203B | ||
| Legionella BCYE Supplement + | 10 tubes (500 mL) Dehydrated culture media | SR0251C | ||
| Legionella MWY Selective Supplement | 10 tubes (500 mL) Dehydrated culture media | SR0255B | ||
| Subculture | Legionella BCYE Agar without Cysteine | Legionella BCYE Agar without Cysteine | 10 x 90 mm plates Prepared media | PO5028A |
| 10 x 90 mm plates Prepared media | PO0255A* | |||
| Legionella CYE Agar Base + | 500 g Dehydrated culture media | CM1203B | ||
| Legionella BCYE without Cysteine Supplement | 10 tubes (100 mL) Dehydrated culture media | SR0253A | ||
| Legionella BCYE Agar | Legionella BCYE Agar | 10 x 90 mm plates Prepared media | PO5072A | |
| Legionella CYE Agar Base + | 500 g Dehydrated culture media | CM1203B | ||
| Legionella BCYE Supplement | 10 tubes (500 mL) Dehydrated culture media | SR0251C |
* Minimum order quantity required.
Detection of Legionella: qualyfast® qPCR
| Product | Format | Order code |
|---|---|---|
| qualyfast Reagents and PCR Kits | ||
| qualyfast Free DNA Inactivator | 200 reactions | D401009 |
| qualyfast DNA Extraction Kit I clear water | 24 reactions | D101001 |
| 48 reactions | D201001 | |
| 96 reactions | D301001 | |
| qualyfast DNA Extraction Kit II dirty water | 48 reactions | D211001 |
| 96 reactions | D311001 | |
| qualyfast Legionella PCR Assay | 24 reactions (3x 8-tube strips) | A101013 |
| 48 reactions (6x 8-tube strips) | A201013 | |
| 96 reactions (12x 8-tube strips) | A301013 | |
| qualyfast Legionella Quantification Standards | 8 tests (4 standards each in an 8-tube strip) | A501013 |
| QuantStudio 5 Food Safety PCR System | ||
| Applied Biosystems™ QuantStudio 5 Food Safety Real-Time PCR System, 0.1 mL Block, Laptop | System | A36328 |
| Applied Biosystems QuantStudio 5 Food Safety Real-Time PCR System, 0.1 mL Block, Desktop | System | A36320 |
| Service & Support Packages | ||
| Applied Biosystems Assurance Service Plan additional 1 year warranty cover with instrument loan | 1 year cover | SCQS5FAST |
Filter Funnels and Membranes
Thermo Scientific™ Nalgene™ Membrane Filters are cellulose nitrate membranes that are certified for microbiological QC testing and analysis of water.
| Product | Size of Quantity | Catalog Number |
|---|---|---|
| Nalgene Disposable Analytical Funnels equipped with membrane filter | ||
| Sterile Analytical Filter Units, 100 mL, 47 mm, 0.45 μm, white/black | 50 pieces | NG145-0045 |
| Sterile Analytical Filter Units, 250 mL, 47 mm, 0.45 μm, white/black | 50 pieces | NG145-2045 |
| Sterile Analytical Filter Units, 100 mL, 47 mm, 0.45 μm, grey/black | 50 pieces | NG147-0045 |
| Sterile Test Filter Funnel, 250 mL, 0.45 uL | 50 pieces | NG147-2045 |
| Membrane filter | ||
| Membrane Filter for water testing, sterile, CN, 47 mm, 0,45 μm, white/black | 100 pieces | NG0205-4045 |
| Membrane Filter for water testing, sterile, CN, 47 mm, 0,45 μm, grey/black | 100 pieces | NG0210-6045 |
| Accessories | ||
| Vacuum Manifold | 1 piece | NG0345-0001 |
| Filter Stopper, non-sterile, no. 8 rubber | 3 pieces | NG0396-0080 |
| Filter Forceps, bent tip | 1 piece | NG0399-0001 |
| Filter Forceps, straight tip | 1 piece | NG0399-0002 |
| Filter Funnel Adapter, non-sterile | 25 pieces | NG0397-0010 |
| Vacuum Gasket, non-sterile thermoplastic elastomer | 6 pieces | NG0395-0708 |
| Nalgene Reusable Analytical Filters and accessories | ||
| Filter Funnels with Clamp, 250 mL | 1 piece | NG0315-0047 |
| Filter Holders with Receiver, 500 mL, 500 mL | 4 pieces | NG0300-4000 |
| Filter Holders with Receiver, 250 mL, 250 mL | 4 pieces | NG0300-4050 |
| Filter Holders with Receiver, 500 mL, 1000 mL | 4 pieces | NG0300-4100 |
| Filter Holders with Funnel, 250 mL | 1 piece | NG0310-4000 |
| Filter Holders with Funnel, 500 mL | 1 piece | NG0310-4050 |
| Reusable Bottle Top Filters, 250 mL | 1 piece | NG0320-2545 |
| Reusable Bottle Top Filters, 500 mL | 1 piece | NG0320-5033 |
| Reusable Bottle Top Filters, 500 mL | 1 piece | NG0320-5045 |
Quality Control organisms: ISO 11133:2014
| Product | Sample numbers | Catalog Number |
|---|---|---|
| Escherichia coli QC Organisms for testing of CCA Agar | ||
| Escherichia coli ATCC® 8739™* | WDCM 00012 | R4607085 |
| Escherichia coli ATCC® 25922™* | WDCM 00013 | R4607050 |
| Enterobacter aerogenes ATCC® 13048™* | WDCM 00175 | R4607080 |
| Pseudomonas aeruginosa ATCC® 27853™* | WDCM 00025 | R4607060 |
| Pseudomonas aeruginosa ATCC® 9027™* | WDCM 00026 | R4605210 |
| Enterococcus faecalis ATCC® 19433™* | WDCM 00009 | R4601990 |
| Enterococcus faecalis ATCC® 29212™* | WDCM 00087 | R4607030 |
| Enterococci QC Organisms for testing of Slanetz and Bartley Agar | ||
| Escherichia coli ATCC® 8739™* | WDCM 00012 | R4607085 |
| Escherichia coli ATCC® 25922™* | WDCM 00013 | R4607050 |
| Staphylococcus aureus subsp. aureus ATCC® 6538™* | WDCM 00032 | R4607016 |
| Staphylococcus aureus subsp. aureus ATCC® 25923™* | WDCM 00034 | R4607010 |
| Enterococcus faecalis ATCC® 29212™* | WDCM 00087 | R4607030 |
| Enterococcus faecalis ATCC® 19433™* | WDCM 00009 | R4601990 |
| Pseudomonas aeruginosa QC Organisms for testing of Pseudomonas CN Agar | ||
| Pseudomonas aeruginosa ATCC® 27853™* | WDCM 00025 | R4607060 |
| Pseudomonas aeruginosa ATCC® 9027™* | WDCM 00026 | R4607060 |
| Pseudomonas aeruginosa ATCC® 10145™* | WDCM 00024 | R4607065 |
| Escherichia coli ATCC® 8739™* | WDCM 00012 | R4607085 |
| Escherichia coli ATCC® 25922™* | WDCM 00013 | R4607050 |
| Enterococcus faecalis ATCC® 19433™* | WDCM 00009 | R4601990 |
| Enterococcus faecalis ATCC® 29212™* | WDCM 00087 | R4601990 |
| Clostridium perfringens QC Organisms for testing of TSC Agar | ||
| Bacillus subtilis ATCC 6633 | WDCM 00003 | R4601221 |
| QC organisms for testing GCVP Agar (Legionella method) | ||
| Legionella pneumophila ATCC® 33152™* | WDCM 00107 | R4603950 |
| Legionella anisa NCTC 11974™ | WDCM 00106 | R4601315 |
| Enterococcus faecalis ATCC® 19433™* | WDCM 00009 | R4601990 |
| Enterococcus faecalis ATCC® 29212™* | WDCM 00087 | R4607030 |
| Pseudomonas aeruginosa ATCC® 27853™* | WDCM 00025 | R4607060 |
| Pseudomonas aeruginosa ATCC® 9027™* | WDCM 00026 | R4605210 |
| Escherichia coli ATCC® 8739™* | WDCM 00012 | R4607085 |
| Escherichia coli ATCC® 25922™* | WDCM 00013 | R4607050 |
| Prepared Media for the Quality Control testing according to ISO 11133:2014 | ||
| CASO Agar (ISO 11133) | PO5321A | |
* The ATCC Licensed Derivative Emblem, the ATCC Licensed Derivative word mark, and the ATCC catalog marks are trademarks of ATCC. Remel Inc. is licensed to use these trademarks and sell products derived from ATCC® cultures. The identity, purity, and authenticity of the Licensed Products are exclusively the responsibility of Remel Inc., and not ATCC.