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Tissue macrophages can be derived from monocytes. When isolated from blood and cultured in media with serum, adherent monocytes will differentiate into macrophages. For a pure macrophage culture, we recommend that you add factors such as M-CSF. Adding other factors including IL-4, IL-10, or TGF-β can help improve viability. The protocol below is for a T25 or T75 flask but can be scaled down for 100 mm culture dishes or plates.
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For Research Use Only. Not for use in diagnostic procedures.