Physical properties that define fluorescence

Learn about basic properties of light, fluorophore excitation, emission, and Stokes shift.


How fluorescence microscopy works

Understand advantages of fluorescence microscopy compared to brightfield, the differences between magnification and resolution, and how using fluorescence can help increase resolution.

Epifluorescence microscope basics

Learn about the light path in typical epifluorescence microscopes and their limits of resolution

Using filters to capture your signal

Understand why filters are used and where in the light path you will find them.

Understanding your objective

Understand what the code on your objective means, why numerical aperture matters, and ways to make sure your microscope configuration will work for your experiment

Now you can take the Molecular Probes School of Fluorescence Basics of Imaging: Chapter 1—Fundamentals of fluorescence microscopy with you on your devices.

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For Research Use Only. Not for use in diagnostic procedures.