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| Affordable & pure, flow-compatible cells | Highest purity for molecular assays | Untouched—most physiological relevant cells | |||
|---|---|---|---|---|---|
| Isolation method | Positive isolation | Depletion or positive isolation | Negative isolation | ||
| Starting samples | Any sample (whole blood, spleen, lymph node) | Any sample (whole blood, spleen, lymph node) | Spleen, lymph node cells* | ||
| # cells processed | 2 x 109 cells | 1–2 x 109 cells | 1 x 109 cells | ||
| Purity | Higher >90% | Higher >90% | High >85% | ||
| Yield | Standard >55% | Highest >95% | High >70% | ||
| Viability | >90% | >90% | >90% | ||
| Isolation time | ~60min | ~30 min | ~50 min | ||
| Isolation temperature | cold | cold | cold | ||
| Applications | Cellular assays (cell culture, cell activation/proliferation, cytokine studies) or molecular isolation of proteins & nucleic acids | Depletion of unwanted T cells or positive isolation for molecular isolation of proteins & nucleic acids | Cellular applications for physiological studies (functional studies, adoptive transfer, flow analysis, or further sorting) | ||
| Bead-free target cells | Yes (but antibody present) | No | No | ||
| Compatible with flow cytometry /flow sorting | Yes | No | Yes | ||
| Shelf life from production date | 2 years | 3 years | 3 years | ||
| Required materials | Magnets | Magnets | Magnets | ||
| Order now | Order now | Order now |
* Lyse the red cells prior to use to improve the purity
The Dynabeads FlowComp Mouse Pan T kit allows you to isolate flow-compatible mouse Pan T (CD90.2+) cells from splenocytes or lymph node cells. Immediately after positive isolation, the beads are released and removed from the cell sample. This avoids any influence of the beads to the cells. The sample cells will not be exposed to the stress of being passed through a column. The result is purity levels of 96-99% of mouse Pan T cells.
Dynabeads Mouse Pan T (Thy 1.2) are magnetic beads that isolate or deplete mouse T cells directly from any sample including whole blood, tissue digests such as spleen or lymph nodes, bone marrow, and mononuclear cells (MNC). Achieve rapid isolation of mouse T cells from any sample with no columns required.
Dynabeads Untouched Mouse T Cells contains magnetic beads and an Antibody Mix to deplete all leucocytes from mouse spleen or lymph node cells, leaving only the target mouse T cells. The negatively isolated, untouched mouse T cells are pure, viable, and ready to use in any downstream application.
THIS IS FOR MOUSE RESEARCH - NOT FOR HUMAN