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Immunophenotyping Examples
This section provides different examples of immunophenotyping data that can be found in application notes, articles, scientific posters, on demand webinars and more.
Immunophenotyping examples features
Application note
Validated 10-color T cell panel using the Attune NxT flow cytometer
In this application note, we take advantage of the OMIP-009 publication, which is optimized for characterizing the immunological response of human T cells, applying it to the 4-laser, 16-parameter configuration of the Invitrogen Attune NxT Flow Cytometer with Autosampler. Additionally, we discuss the importance of antibody titration and optimizing antibody concentration in panel testing. Furthermore, we review the importance of fluorescence minus one (FMO) controls.
Application note
Attune NxT Flow Cytometer for 6-color immunophenotyping analysis of stained human whole blood using a no-lyse, no-wash protocol, with no compensation
This application note demonstrates how to set up a multicolor flow cytometry experiment so that optimal dyes are selected. Additionally, the criteria for setting up a no compensation panel are described.
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| Type | Title | Categories |
|---|---|---|
| Application note (2017) | Validated 10-color T cell panel using the Attune NxT Flow Cytometer | antibodies, Attune/Attune NxT Flow Cytometer, flow cytometry, immunology, immunophenotyping, fluorescent dyes, multicolor flow cytometry |
| Application note (2017) | Detection of platelets in whole blood using the Attune NxT Flow Cytometer | Attune NxT, flow cytometer, platelets, immunophenotyping, no-wash, no-lyse |
| Application note (2015) | Attune NxT Flow Cytometer for 6-color immunophenotyping analysis of stained human whole blood using a no-lyse, no-wash protocol, with no compensation | Attune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry |
| Application note (2015) | Detection of murine regulatory T cells on the Attune NxT Flow Cytometer | Attune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry |
| Application note (2015) | Multiparameter analysis of murine regulatory T cells and dendritic cells with the Attune NxT Flow Cytometer | Attune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry |
| Application note (2015) | Ten-parameter immunophenotyping of human lysed whole blood with the Attune NxT Flow Cytometer allows identification of multiple T cell subsets and myeloid cells | Attune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry |
| Application note (2015) | 13-parameter immunophenotyping of human lysed whole blood with the Attune NxT Flow Cytometer allows for the identification of B cells, NK cells, multiple T cell subsets and myeloid cells | Alexa Fluor, antibodies, Attune NxT, compensation, flow cytometer, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot |
| BioProbes article (2018) | Immuno-oncology: Advances in Basic Research and Translational Medicine. With a focus on immune checkpoint inhibitors and T cell immunotherapy | antibodies, ELISA, flow cytometry, fluorescence microscopy/fluorescence imaging, high content analysis (HCA), immunohistochemistry (IHC), immunology, immuno-oncology, immunophenotyping, Luminex, ProCartaPlex, spheroids |
| BioProbes article (2016) | Transcription factor expression during differentiation of hPSC-derived cardiomyocytes—A multiparametric approach using the Attune NxT Flow Cytometer | Attune NxT, flow cytometer, fluorescence, large cell analysis, rare event detection, stem cell research |
| BioProbes article (2016) | Optimized multicolor immunofluorescence panels (OMIPs)—Jump-start your experimental design with published antibody and reagent panels | flow cytometry, fluorescence, immunophenotyping, multicolor flow cytometry |
| BioProbes article (2015) | No-compensation immunophenotyping using a four-laser flow cytometer | Attune NxT, flow cytometer, fluorescence, immunophenotyping, multicolor flow cytometry |
| Guided learning | T cell stimulation and proliferation eLearning course | antibodies, cell proliferation, cell stimulation, flow cytometry, immunoassays, immunology, immunophenotyping, T cells |
| Scientific Poster (2015) | A no lyse, no-wash approach to characterizing phagocyte phenotype and function in whole human blood on the Attune NxT Flow Cytometer | Attune NxT, cell cycle, flow cytometer, flow cytometry assays, immunophenotyping, no lyse, phagocytosis, whole blood |
| Scientific poster (2010) | Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry and imaging | Alexa Fluor, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, immunofluorescence (IF), immunophenotyping, stem cell research, violet laser-excited reagents |
| Scientific poster (2009) | Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry | Alexa Fluor, cell proliferation, cells, Click-iT, flow cytometry, fluorescent dyes, fluorescent proteins, immunophenotyping, nucleic acid quantitation, stem cell research, violet laser-excited reagents |
| CYToMORE lecture video (2016) | Assessing immunological aging using flow cytometry Presented by Victor Appay, Faculté de Médecine Pierre et Marie Curie, Pitié-Salpêtrière, France | flow cytometry, immunology, immunophenotyping |
| CYToMORE lecture video (2016) | Cytometric analysis of cytokines and transcription factors Presented by Hyun-Dong Chang, Deutsches Rheuma-Forschungszentrum, Berlin, Germany | cell signaling, flow cytometry, immunophenotyping |
| CYToMORE lecture video (2016) | Heterogeneity of tumour-infiltrating leukocytes: implications for adoptive cell transfer immunotherapy Presented by Enrico Lugli, Humanitas Clinical and Research Center, Italy | flow cytometry, immunology, immunophenotyping |
| CYToMORE lecture video (2016) | Mass cytometry in the phenomics era: an essential tool for gene/function studies Presented by Herve Luche, Centre d’Immunophénomique - CIPHE, France | mass cytometry, immunology, immunophenotyping |