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Super Bright 702 Antibody Conjugates
Comparable to Brilliant Violet 711 conjugates
Invitrogen eBioscience Super Bright 702 antibody conjugates for flow cytometry provide:
- Less spill over into certain blue channels and also into other violet channels, including the Brilliant Violet™ 786 detection channel
- Options in marker and clone selection for violet laser excitable antibody conjugates
- Compatibility with standard intracellular buffers, viability stains, compensation beads, and other antibodies
Multiplexing with Super Bright 702 antibody conjugates
Figure 1. Fluorescence intensity comparison of Super Bright 702 conjugates to Brilliant Violet™ 711 conjugates.(A) Mouse splenocytes stained with anti-CD4 conjugated to Super Bright 702 (red) or Brilliant Violet™ 711 conjugate (gray), at the same concentration of antibody. (B) Human peripheral blood cells stained with anti-CD19 conjugated to Super Bright 702 (red) or Brilliant Violet 711™ conjugate (gray), using the same concentration of antibody.
| Multiplexing compatibility | Buffer | Multiplexing considerations |
|---|---|---|
| Multiplexing with 1 Super Bright antibody conjugate | Standard buffers applicable | No special buffer required when only one Super Bright antibody conjugate is used in a panel |
| Multiplexing with 2 or more Super Bright antibody conjugates | Super Bright Staining Buffer | Special staining buffer is required prior to addition of Super Bright conjugates to reduce non-specific dye-dye interactions |
| Multiplexing with 1 or more Brilliant Violet™ antibody conjugates | Super Bright Staining Buffer | Special staining buffer is required to reduce non-specific dye-dye interactions. Use of the Super Bright Staining Buffer is recommended, but the similar Brilliant Stain Buffer can be substituted |
Figure 2. 10-color ILC2 subset panel. Normal human PBMCs were surface-stained in the presence of Super Bright Staining Buffer (Cat. No. SB-4400-42) at optimal concentrations for the indicated surface markers.(A) Gated on live cells, this plot shows the lineage markers used as a FITC dump channel (CD3 (clone UCHT1), CD4 (clone SK3), CD8a (clone RPA-T8), CD11b (clone ICRF44), and CD19 (clone HIB19) vs. CD127 (IL-7RA) (clone eBioRDR5) Super Bright 436 (Cat. No. 62-1278-42) stained cells. Since ILC2 subsets are negative for all five of these markers, all CD127 and lineage-positive cells can be eliminated from further analysis. (B-G) Gating strategy is shown for all lineage targets to highlight the ILC2 population. (H, I) The CD294 (CRTH2) population is identified.
| Viability stain options | Product | Multiplexing considerations |
|---|---|---|
| Fixable | LIVE/DEAD fixable dead cell stain kits | No compatibility concerns |
| Non-fixable | SYTOX non-fixable dead cell stains Ready Flow Ready-to-use viability reagents | No compatibility concerns Compatible with all Ready Flow reagents for viability |
| Product | Multiplexing considerations |
|---|---|
| UltraComp eBeads microspheres | UltraComp eBeads microspheres are compatible but OneComp eBeads are not compatible with violet lasers; the AbC Total Antibody Compensation Bead Kit is also compatible with the Super Bright antibody conjugates. |
| Staining Target | Product | Multiplexing considerations |
|---|---|---|
| Cytosolic staining (cytokines) | Intracellular Fixation & Permeabilization Buffer Set | No compatibility concerns |
| Nuclear staining (transcription factors) | Foxp3/Transcription Factor Staining Buffer Set | No compatibility concerns |
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Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.