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We are proud to provide a new product line surface treatment that will support improved cell yield and morphology as well enabling you to do xeno-free or low-serum applications.
See how our surfaces perform with 39 cell types and cell lines at thermofisher.com/supradata
We’ve tested the most-used cell lines on Thermo Scientific Nunclon Delta, Supra and Sphera cell culture plastics side-by-side with the leading competitor to validate Nunc cell culture plastics’ place in your cell culture hood.
Please note: We are adding data for more cells all the time.
A431 cells grown on Nunc Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.
Below are representative brightfield images of A431 cells grown on Nunclon Delta surfaces (left) and Corning® tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
The below image describes A431 cells grown on Nunclon Delta plates or Corning® TC surfaces for at least 10 passage doublings were assayed for cell health with respect to expression of two common endogenous genes, EGFR (epidermal growth factor receptor) and VEFGA (vascular endothelial growth factor alpha). The normalized relative gene expression of cells grown on Corning TC surface with respect to Nunclon Delta surfaces surface is shown on the y-axis.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
Please note: For the above A431 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco IMDM, 10% Gibco FBS, and 1% Penicillin Streptomycin.
A549 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.
Below are representative brightfield images of A549 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
A549 cells grown on Nunclon Delta surfaces (Nunc) or Corning TC (Corning) surfaces as well as those switched to Nunclon Delta surfaces (S to ND) surface, were treated with epidermal growth factor (EGF, 200 ng/mL) for 10 minutes. Total cell lysates (30 mg) were subjected to western blot analysis for EGFR phosphorylation using anti-phospho-EGFR (Tyr1086) rabbit polyclonal antibody 0.5 µg/mL. Total EGFR and GAPDH were used as experimental and normalization controls respectively.
Please note: For the above A549 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco IMDM, 10% Gibco FBS, and 1% Penicillin Streptomycin.
Caco-2 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.
Below are representative brightfield images of Caco-2 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
As an extension of cell attachment and growth, Caco-2 cells grown on Nunclon Delta surfaces (left) and Corning® TC surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface (right) were stained for the tight junction protein ZO-3, shown in green. The nucleus was stained with DAPI (shown in blue). No morphological difference was observed between the surfaces. Cells were grown on 6-well plates and stained on the same surface. Images were captured using EVOS M5000 imaging system under 20X magnification and cropped using the same aspect ratio for better visualization.
Please note: For the above Caco-2 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 20% Gibco FBS, and 0.5% Penicillin Streptomycin.
CHO-K1 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.
Below are representative brightfield images of CHO-K1 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
CHO-K1 cells seeded on Nunclon Delta surfaces and Corning TC surface (6-well plates) and transfected with mCherry using Neon Transfection System. Representative images of live cells were acquired 24 hours post transfection.
Please note: Flow cytometry evaluation of transfection efficiency of mCherry in CHO-K1 cells: 10,000 events were captured per sample. UT=Untransfected cells, T=Transfected cells. n=2.
Please note: For the above CHO-K1 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.
Cos-7 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.
Below are representative brightfield images of Cos-7 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
Please note: For the above Cos-7 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.
HEK293 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.
Below are representative brightfield images of HEK293 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
HEK293 cells seeded on Nunclon Delta surfaces or Corning TC surfaces and transfected with mCherry using Lipofectamine 3000 transfection reagent. Images of live cells were acquired 24 hours post transfection.
Please note: Flow cytometry evaluation of transfection efficiency of mCherry in HEK293 cells: 10,000 events were captured per sample. UT=Untransfected cells, T=Transfected cells. n=2.
Please note: For the above HEK293 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.
HeLa cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.
Below are representative brightfield images of HeLa cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
HeLa cells seeded on Nunclon Delta surfaces or Corning TC surfaces (6-well plates) and transfected with mCherry using Lipofectamine 3000 transfection reagent. Images of live cells were acquired 24 hours post transfection.
Please note: Flow cytometry evaluation of transfection efficiency of mCherry in HeLa cells: 10,000 events were captured per sample. UT=Untransfected cells, T=Transfected cells. n=2.
Please note: For the above HeLa Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.
HepG2 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.
Below are representative brightfield images of HepG2 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
HepG2 cells grown on Nunclon Delta surfaces or Corning TC surfaces for at least 10 passage doublings were assayed for cell health with respect to expression of two common endogenous genes, SLCO2B1 (solute carrier organic anion transporter family member 2B1) and ABCC6 (multidrug resistance associated protein 6). The normalized relative gene expression of cells grown on Corning TC surface with respect to Nunclon Delta surfaces surface is shown on the y-axis.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
Please note: For the above HepG2 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.
HT-29 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.
Below are representative brightfield images of HT-29 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
Please note: For the above HT-29 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco IMDM, 10% Gibco FBS, and 1% Penicillin Streptomycin.
MCF-7 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.
Below are representative brightfield images of MCF-7 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
MCF-7 cells grown on Nunclon Delta surfaces (left) or Corning TC surfaces (middle) as well as those switched to Nunclon Delta surfaces surface (right) were analyzed for the expression of endogenous receptor tyrosine protein kinase ErbB2 using flow cytometry. IgG1 was used as the isotype control. No difference was observed in the relative expression of ErbB2 using the different growth surfaces. Samples were run in duplicate on an Invitrogen Attune NxT Flow Cytometer.
Please note: For the above MCF-7 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco MEM, 10% Gibco FBS, 1% Penicillin Streptomycin, and 0.01 mg/ml Insulin.
MCF-10A cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.
Below are representative brightfield images of MCF-10A cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
MCF-10A cells grown on Nunclon Delta surfaces or Corning TC surfaces for at least 10 passage doublings were assayed for the expression of cell surface associated MUC1 (mucin-1) gene by qPCR. RNA was isolated using RiboPure RNA Purification Kit, cDNA was prepared using High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor, and qPCR was performed using TaqMan Fast Advanced Master Mix in a Custom TaqMan Array Plate. The normalized relative gene expression of cells grown on Corning TC surface with respect to Nunclon Delta surfaces surface is plotted on the y-axis.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
Please note: For the above MCF-10A Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Reconstituted Gibco Medium 171, 100 ng/ml cholera toxin, and 1% Penicillin Streptomycin.
MDA-MB-231 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.
Below are representative brightfield images of MDA-MB-231 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
MDA-MB-231 cells grown on Nunclon Delta surfaces or Corning TC surface for 25 doublings as well as those switched to Nunclon Delta surfaces (ND) surface for 10 doublings were assessed for the expression of two endogenous surface marker proteins: ICAM-1 (top row, 0.25 μg/test) and CD44 (bottom row, 0.06 μg/test) using flow cytometry. 10,000 events were collected for each condition. No difference was observed in the surface marker expression between different growth surfaces.
Please note: For the above MDA-MB-231 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.
MDCK cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.
Below are representative brightfield images of MDCK cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
As an extension to cell attachment, MDCK cells grown on Nunclon Delta surfaces or Corning TC surfaces for at least 10 passage doublings were assayed for the gene expression of endogenously expressed CDH-1 (cadherin-1), a cell adhesion marker. Cells were also assayed for expression of the characteristic endogenous gene MUC1 (mucin-1) by qPCR. RNA was isolated using RiboPure RNA purification kit, cDNA was prepared using a cDNA preparation kit, and qPCR was performed using TaqMan Assay Master Mix in a custom TaqMan array plate. The normalized relative gene expression of cells grown on Corning TC surface with respect to Nunclon Delta surfaces surface is plotted on the y-axis.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
Please note: For the above MDCK Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.
NIH3T3 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.
Below are representative brightfield images of NIH3T3 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
Please note: For the above NIH3T3 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.
PC-3 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.
Below are representative brightfield images of PC-3 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
PC-3 cells seeded on Nunclon Delta surfaces or Corning tissue culture (TC) treated surfaces and transfected with mCherry using Lipofectamine 3000 transfection reagent. Images of live cells were acquired 24 hours post transfection.
Please note: Flow cytometry evaluation of transfection efficiency of mCherry in PC-3 cells: 10,000 events were captured per sample. UT=Untransfected cells, T=Transfected cells. n=2.
Please note: For the above PC-3 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.
HepG2 cells grown on Nunclon Sphera surface, in combination with Gibco cell culture media and fetal bovine serum have been proven to yield consistent, quality spheroids.
In a side-by-side comparison with a leading supplier, Nunclon Sphera yielded high-quality HepG2 spheroids, while the other supplier’s product yielded undesirable satellite colonies.
Spheroid formation in Nunclon Sphera and Corning® ULA™ U-bottomed surface: HepG2 cells were seeded at the respective densities on Nunclon Sphera and Corning ULA U-bottomed plates for spheroid generation. On Day 6, brightfield images of spheroids were captured with EVOS imaging system using 4X objective. The figure shows representative images. Spheroids formed on the Sphera surface had no satellite colonies, while those grown on Corning ULA plates were found to have satellite colonies at higher seeding densities. Scale bar=1,000 μm.
LNCaP cells grown on Nunclon Sphera surface, in combination with Gibco cell culture media and fetal bovine serum have been proven to yield consistent, quality spheroids.
In a side-by-side comparison with a leading supplier, Nunclon Sphera yielded high-quality LNCaP spheroids, while the other supplier’s product yielded undesirable satellite colonies.
Spheroid formation in Nunclon Sphera and Corning ULA U-bottomed surface: LNCaP cells were seeded at the respective densities on Nunclon Sphera and Corning ULA U-bottomed plates for spheroid generation. On Day 6, brightfield images of spheroids were captured with EVOS imaging system using 4X objective. The figure shows representative images. Spheroids formed on the Sphera surface had no satellite colonies, while those grown on Corning ULA plates were found to have satellite colonies at higher seeding densities. Scale bar=1,000 μm.
T47D cells grown on Nunclon Sphera surface, in combination with Gibco cell culture media and fetal bovine serum have been proven to yield consistent, quality spheroids.
In a side-by-side comparison with a leading supplier, Nunclon Sphera yielded high-quality T47D spheroids, while the other supplier’s product yielded undesirable satellite colonies.
Spheroid formation in Nunclon Sphera and Corning ULA U-bottomed surface: T47D cells were seeded at the respective densities on Nunclon Sphera and Corning ULA U-bottomed plates for spheroid generation. On Day 6, brightfield images of spheroids were captured with EVOS imaging system using 4X objective. The figure shows representative images. Spheroids formed on the Sphera surface had no satellite colonies, while those grown on Corning ULA plates were found to have satellite colonies at higher seeding densities. Scale bar=1,000 μm.
For Research Use Only. Not for use in diagnostic procedures.