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KingFisher Software and Protocols
BindIt Software is free and provides automated protocols for KingFisher systems
KingFisher automated purification systems are designed to adapt to your changing research needs. That’s why we include free software with all KingFisher instruments so that you can create, modify and store your protocols. You can also search and download automated protocols for our MagMAX nucleic acid purification kits, and Dynabeads and Pierce protein purification beads and kits.
Features of Software
- Compatible with KingFisher instruments
- Conveniently create and modify protocols on your PC and transfer to them to your KingFisher instrument. The software allows you to define and edit steps and parameters for every part of your protocol—bind, wash, elute
- Allows KingFisher plates and reagents to be defined in the plate layout
- Generates status reports that include run log, plate layout, and step parameters
Instructions for downloading and installing protocols with BindIt software:
- Make sure you have BindIt 4.1 Software installed in your computer
- Click the protocol in the table (DNA/RNA protocols, Protein protocols). Protocol opens directly in BindIt Software
- Save the protocol in your computer in the preferred folder.
- Connect your PC with USB cable to KingFisher instrument and select Home > Connect in BindIt Software.
- You can start the protocol from the BindIt or transfer it into instrument. If you transfer the protocol select Home > Transfer in BindIt Software.
- Select Upload and find the saved protocol. Click open.
- Select the folder (category) you want to store the protocol in the instrument.
- Your protocol can be now found in the instrument memory.
- Disconnect your instrument from BindIt and check using keypad that you protocol is in the desired folder.
Already have a KingFisher Instrument and BindIt Software?
BindIx for
KingFisher Apex
Features of Software
- All of the functionality of the Apex instrument on your PC
- Conveniently create and modify protocols
- Easily transfer new or edited protocols to KingFisher Apex
- Access protocol library
- Available for PC laptops or desktop computers
- Compatible with KingFisher Apex instruments only
How to install BindIx software*?
- Download BindIx here
- Download BindIx from Microsoft Store here
BindIx from Microsoft Store can be installed by selecting “Install” button on MS Store. - USB stick (delivered from manufacture)
BindIx from USB stick can be installed by double-clicking .msix bundle file and after that selecting “Install” button.
Note: System requirements for BindIx software is Windows 10 version 1809 (17763.0) or higher.
DNA & RNA extraction protocols available to download by SKU
| Nucleic acid | Sample type | Kit name | SKU | Format | Software protocol |
|---|---|---|---|---|---|
| RNA | Saliva | MagMAX Viral/Pathogen Kit (MVP I) | A48310 A42352 | up to 2,000 preps up to 200 preps | MagMAX Viral/Pathogen RNA Saliva Flex |
| MagMAX Viral/Pathogen II Kit (MVPII) | A48383 | up to 2,000 preps | |||
| DNA | Blood, buccal, swabs | MagMAX DNA Multi-Sample Ultra | A25597 A25598 | 500 preps 2,500 preps | MagMAX DNA Blood Buccal Duo Prime Flex |
| DNA | Blood, buccal, swabs | MagMAX DNA Multi-Sample Ultra w/ extra PK and beads | A25919 A25920 | 500 preps 2,500 preps | MagMAX DNA Blood Buccal Duo Prime Flex |
| DNA | Blood, buccal, swabs | MagMAX DNA Multi-Sample | 4413020 | 50 preps | MagMAX DNA Blood Buccal Duo Prime Flex |
| DNA | Blood, buccal, swabs | MagMAX-96 DNA Multi-Sample | 4413021 4413022 | 96 preps 5 x 96 preps | MagMAX DNA Blood Buccal Duo Prime Flex |
| DNA | PCR reaction | ChargeSwitch PCR Clean-Up Kit | CS12000 CS1200010 | 100 preps 960 preps | ChargeSwitch PCR reaction Flex |
| DNA | Tissue, cells | MagMAX DNA Multi-Sample Ultra | A25597 A25598 | 500 preps 2,500 preps | MagMAX DNA tissue cells Duo Prime Flex |
| DNA | Tissue, cells | MagMAX DNA Multi-Sample Ultra w/ extra PK and beads | A25919 A25920 | 500 preps 2,500 preps | MagMAX DNA tissue cells Duo Prime Flex |
| DNA | Tissue, cells | MagMAX DNA Multi-Sample | 4413021 | 50 preps | MagMAX DNA tissue cells Duo Prime Flex |
| DNA | Tissue, cells | MagMAX-96 DNA Multi-Sample | 4413021 4413022 | 96 preps 5 x 96 preps | MagMAX DNA tissue cells Duo Prime Flex |
| RNA/DNA | Viral | MagMAX Pathogen RNA/DNA | 4462359 | 480 preps | MagMAX Pathogen Std Volume Duo Prime Flex |
| RNA/DNA | Viral | MagMAX Viral RNA | AM1836 | 96 preps | MagMAX Pathogen Std Volume Duo Prime Flex |
| RNA/DNA | Viral | MagMAX Total Nucleic Acid | AM1840 | 100 preps | MagMAX Pathogen Std Volume Duo Prime Flex |
| RNA/DNA | Viral | MagMAX Pathogen RNA/DNA | 4462359 | 480 preps | MagMAX Pathogen High Volume Duo Prime Flex |
| RNA/DNA | Viral | MagMAX Viral RNA | AM1836 | 96 preps | MagMAX Pathogen High Volume Duo Prime Flex |
| RNA/DNA | Viral | MagMAX Total Nucleic Acid | AM1840 | 100 preps | MagMAX Pathogen High Volume Duo Prime Flex |
| RNA | Viral | MagMAX-96 AI/ND Viral RNA Isolation Kit | AM1835 | 384 preps | MagMAX AI/ND Viral RNA Duo Prime Flex |
| RNA/ microRNA | Tissue, cells blood, plasma, urine | MagMAX mirVana Total RNA | A27828 | 96 preps | MagMAX mirVana tissue cells Duo Prime Flex MagMAX mirVana biofluids Duo Prime Flex |
| DNA | Plasma | MagMAX Cell-Free DNA | A29319 | 96 preps | MagMAX Cell-Free DNA 2mL plasma Duo Prime Flex MagMAX Cell-Free DNA 4mL plasma Duo Prime Flex |
| RNA | Tissue, cells, plants | MagMAX-96 Total RNA | AM1830 | 96 preps | MagMAX Total RNA tissue cell plant Duo Prime Flex |
| RNA | Blood | MagMAX for Stabilized Blood (Paxgene tubes) | 4451894 | 96 preps | MagMAX for Paxgene tubes Duo Prime Flex |
| RNA | Blood | MagMAX for Stabilized Blood (Tempus tubes) | 4451893 | 96 preps | MagMAX for Tempus tubes Duo Prime Flex |
| DNA | Leukocytes | MagMAX DNA Multi-Sample Ultra | A25597 | 500 preps | MagMAX DNA leukocytes Duo Prime Flex |
| mRNA | Dynabeads mRNA Direct | 61011 61012 | 5 mL 10 mL | Dynabeads mRNA from crude samples Duo Prime Flex | |
| RNA | MagMAX for Microarrays Total RNA Isolation | AM1839 | 96 preps | MagMAX for Microarrays with 100uL aqueous phase Duo Prime Flex | |
| DNA | FFPE | MagMAX FFPE DNA/RNA Ultra Kit | A31881 | 1 kit | MagMAX FFPE Ultra DNA only Duo Prime Flex |
| RNA | FFPE | MagMAX FFPE DNA/RNA Ultra Kit | A31881 | 1 kit | MagMAX FFPE Ultra RNA only Duo Prime Flex |
| RNA/DNA | FFPE | MagMAX FFPE DNA/RNA Ultra Kit | A31881 | 1 kit | MagMAX FFPE Ultra DNA and RNA Duo Prime Flex |
Instructions for downloading and installing protocols:
- Make sure you have BindIt 4.1 Software installed in your computer
- Click the protocol in the table. Protocol opens directly in BindIt Software
- Save the protocol in your computer in the preferred folder.
- Connect your PC with USB cable to KingFisher instrument and select Home > Connectin BindIt Software.
- You can start the protocol from the BindIt or transfer it into instrument. If you transfer the protocol select Home > Transfer in BindIt Software.
- Select Uploadand find the saved protocol. Click open.
- Select the folder (category) you want to store the protocol in the instrument.
- Your protocol can be now found in the instrument memory.
- Disconnect your instrument from BindIt and check using keypad that you protocol is in the desired folder.
Immunoprecipitation protocols for KingFisher Flex or DUO Prime automation
† Strategy (Indirect or Direct). This refers to the order of binding steps, and it primarily pertains to immunoprecipitation protocols. Indirect protocols involve incubating the target-containing sample and antibody first, and then incubating with the beads second. Direct protocols involve incubating beads and antibody first (either to bind or covalently couple the IP antibody), and then incubating the bead-antibody complex with the sample.
| Ligand | Product | Protocol name: description | Strategy† | Download file |
|---|---|---|---|---|
| Protein A | DynaGreen Protein A | IP DynaGreen Protein A Direct: Immunoprecipitation (IP or Co-IP) | Direct | Flex Duo Prime |
| Protein A | DynaGreen Protein A | IP DynaGreen Protein A Indirect: Immunoprecipitation (IP or Co-IP) | Indirect | Flex Duo Prime |
| Protein A/G | DynaGreen Protein A/G | IP DynaGreen Protein A/G Direct: Immunoprecipitation (IP or Co-IP) | Direct | Flex Duo Prime |
| Protein A/G | DynaGreen Protein A/G | IP DynaGreen Protein A/G Indirect: Immunoprecipitation (IP or Co-IP) | Indirect | Flex Duo Prime |
| Capture Select | DynaGreen Capture Select | IP DynaGreen CaptureSelect Anti-IgG-Fc Direct: Immunoprecipitation (IP or Co-IP) | Direct | Flex Duo Prime |
| Capture Select | DynaGreen Capture Select | IP DynaGreen CaptureSelect Anti-IgG-Fc Indirect: Immunoprecipitation (IP or Co-IP) | Indirect | Flex Duo Prime |
| Antibodies | FabRICATOR MagIC immobilized on magnetic beads | High throughput parallel analysis of antibodies | Indirect | Flex Duo Prime |
| Protein A | Dynabeads Protein A | IP Dynabeads M-280 Protein A G Direct: Immunoprecipitation (IP or Co-IP) | Direct | Flex Duo Prime |
| Protein A | Dynabeads Protein A | IP Dynabeads M-280 Protein A G Indirect: Immunoprecipitation (IP or Co-IP) | Indirect | Flex Duo Prime |
| Protein A | Dynabeads Protein A Immunoprecipitation Kit | IP Dynabeads M-280 Protein A G Direct: Immunoprecipitation (IP or Co-IP) | Direct | Flex Duo Prime |
| Protein A | Dynabeads Protein A Immunoprecipitation Kit | IP Dynabeads M-280 Protein A G Indirect: Immunoprecipitation (IP or Co-IP) | Indirect | Flex Duo Prime |
| Protein G | Dynabeads Protein G | IP Dynabeads M-280 Protein A G Direct: Immunoprecipitation (IP or Co-IP) | Direct | Flex Duo Prime |
| Protein G | Dynabeads Protein G | IP Dynabeads M-280 Protein A G Indirect: Immunoprecipitation (IP or Co-IP) | Indirect | Flex Duo Prime |
| Protein G | Dynabeads Protein G Immunoprecipitation Kit | IP Dynabeads M-280 Protein A G Direct: Immunoprecipitation (IP or Co-IP) | Direct | Flex Duo Prime |
| Protein G | Dynabeads Protein G Immunoprecipitation Kit | IP Dynabeads M-280 Protein A G Indirect: Immunoprecipitation (IP or Co-IP) | Indirect | Flex Duo Prime |
| Sheep anti-Mouse IgG | Dynabeads Sheep anti-Mouse IgG | IP Dynabeads M-280 SAM SAR Direct: Immunoprecipitation (IP or Co-IP) | Direct | Flex Duo Prime |
| Sheep anti-Rabbit IgG | Dynabeads Sheep anti-Rabbit IgG | IP Dynabeads M-280 SAM SAR Direct: Immunoprecipitation (IP or Co-IP) | Direct | Flex Duo Prime |
| Streptavidin | Dynabeads M-280 Streptavidin | IP Dynabeads M-280 SA Direct: Immunoprecipitation (IP or Co-IP) | Direct | Flex Duo Prime |
| Glutathione | Pierce Glutathione Magnetic Agarose Beads | Purify GST tag Magnetic Agarose GSH Direct: GST fusion protein purification | Direct | Flex Duo Prime |
| Ni-IMAC | Pierce High Capacity Ni-IMAC MagBeads, EDTA-compatible | Purify His tag Magnetic Agarose Ni-IMAC Direct | Direct | Flex Duo Prime |
| Ni-NTA | Pierce Ni-NTA Magnetic Agarose Beads | Purify His tag Magnetic Agarose Ni-NTA Direct: His-tagged fusion protein purification | Direct | Flex Duo Prime |
| Protein A/G | Pierce Protein A/G Magnetic Agarose Beads | Purify Ab Magnetic Agarose Protein AG Direct: Antibody purification of IgG containing Fc region | Direct | Flex Duo Prime |
| Anti-HA Antibody | Pierce Anti-HA Magnetic Beads | IP HA tag Pierce anti-HA Ab Direct: HA-tagged fusion protein purification or immunoprecipitation (IP or Co-IP) | Direct | Flex |
| Anti-c-Myc Antibody | Pierce Anti-c-Myc Magnetic Beads | IP Myc tag Pierce anti-Myc Ab Direct: Myc-tagged fusion protein purification or immunoprecipitation (IP or Co-IP) | Direct | Flex |
| Glutathione | Pierce Glutathione Magnetic Beads | Purify GST tag Pierce GSH Direct: GST fusion protein purification or pull-down | Direct | Flex |
| Ni-NTA | HisPur Ni-NTA Magnetic Beads | Purify His tag Pierce Ni-NTA Direct: His-tagged fusion protein purification or pull-down | Direct | Flex |
| NHS Ester | Pierce NHS-Activated Magnetic Beads | Couple Pierce NHS ester Direct: Coupling (immobilization) of primary amine molecule (protein, peptide) | Direct | Flex |
| NHS Ester | Pierce NHS-Activated Magnetic Beads | IP Pierce NHS ester Direct: Affinity purification with coupled (immobilized) primary amine molecule (protein, peptide) | Direct | Flex |
| Protein A/G | Pierce Protein A/G Magnetic Beads | Purify Ab Pierce Protein AGL Direct: Antibody purification of IgG containing Fc region | Direct | Flex |
| Protein A | Pierce Protein A Magnetic Beads | Purify Ab Pierce Protein AGL Direct: Antibody purification of IgG containing Fc region | Direct | Flex |
| Protein G | Pierce Protein G Magnetic Beads | Purify Ab Pierce Protein AGL Direct: Antibody purification of IgG containing Fc region | Direct | Flex |
| Protein L | Pierce Protein L Magnetic Beads | Purify Ab Pierce Protein AGL Direct: Antibody purification of immunoglobulins containing kappa light chains | Direct | Flex |
| Protein A/G | Pierce Protein A/G Magnetic Beads | IP Pierce Protein AGL Indirect: Immunoprecipitation (IP or Co-IP) | Indirect | Flex |
| Protein A | Pierce Protein A Magnetic Beads | IP Pierce Protein AGL Indirect: Immunoprecipitation (IP or Co-IP) | Indirect | Flex |
| Protein G | Pierce Protein G Magnetic Beads | IP Pierce Protein AGL Indirect: Immunoprecipitation (IP or Co-IP) | Indirect | Flex |
| Protein A/G | Pierce Classic Magnetic IP/Co-IP Kit | IP Pierce Protein AGL Indirect: Immunoprecipitation (IP or Co-IP) | Indirect | Flex |
| NHS Ester | Pierce Direct Magnetic IP/Co-IP Kit | IP Pierce Direct IP Kit Direct: Immunoprecipitation (IP or Co-IP) or pull-down after coupling (immobilization) of IgG, protein or peptide to beads | Direct | Flex |
| Protein A/G | Pierce Crosslink Magnetic IP/Co-IP Kit | IP Pierce Crosslink IP Kit Direct: Immunoprecipitation (IP or Co-IP) after crosslinking IgG to beads | Direct | Flex |
| Protein A/G | Pierce Magnetic ChIP Kit | ChIP Pierce ChIP Kit Indirect: Chromatin immunoprecipitation (ChIP) of protein-DNA complexes | Indirect | Flex |
| Streptavidin | Pierce Streptavidin Magnetic Beads | IP Pierce SA heated elution Indirect: Purification or enrichment of biotinylated or desthiobiotinylated molecules using heated elution | Indirect | Flex |
| Streptavidin | Pierce Streptavidin Magnetic Beads | IP Pierce SA low pH elution Indirect: Purification or enrichment of biotinylated or desthiobiotinylated molecules using low-pH elution | Indirect | Flex |
| Titanium Dioxide (TiO2) | Pierce Magnetic Titanium Dioxide Phosphopeptide Enrichment Kit | Enrich pPep Pierce pPep Kit Direct deepwell plate: Phosphopeptide enrichment in deep-well plates (peptides containing phosphorylated amino acids, e.g., phosphoserine, phosphotyrosine or phosphotyrosine) | Direct | Flex |
| Titanium Dioxide (TiO2) | Pierce Magnetic Titanium Dioxide Phosphopeptide Enrichment Kit | Enrich pPep Pierce pPep Kit Direct PCR plate: Phosphopeptide enrichment in PCR plates (peptides containing phosphorylated amino acids, e.g., phosphoserine, phosphotyrosine or phosphotyrosine) | Direct | Flex |
Instructions for downloading and installing protocols:
- Make sure you have BindIt 4.1 Software installed in your computer
- Click the protocol in the table. Protocol opens directly in BindIt Software
- Save the protocol in your computer in the preferred folder.
- Connect your PC with USB cable to KingFisher instrument and select Home > Connect in BindIt Software.
- You can start the protocol from the BindIt or transfer it into instrument. If you transfer the protocol select Home > Transfer in BindIt Software.
- Select Upload and find the saved protocol. Click open.
- Select the folder (category) you want to store the protocol in the instrument.
- Your protocol can be now found in the instrument memory.
- Disconnect your instrument from BindIt and check using keypad that you protocol is in the desired folder.
Dynabeads virus enrichment and positive cell isolation protocols to download
| Ligand | Product | Protocol description | Strategy | Download file |
|---|---|---|---|---|
| Virus | Viruses (especially SARS-CoV-2), vesicles and proteins | Dynabeads Intact Virus Enrichment | Indirect | Duo Prime |
| Virus | Viruses (especially SARS-CoV-2), vesicles and proteins | Dynabeads Intact Virus Enrichment | Indirect | Flex |
| Cells | CD3, CD4, CD8, CD14, CD19 | Positive Cell Isolation for downstream nucleic acid, protein analysis, or cell depletion | Indirect | Duo Prime |
| Cells | CD3, CD4, CD8 | Positive Cell Isolation for downstream cellular applications – such as T-cell Activation | Indirect | Duo Prime |
Instructions for downloading and installing protocols:
- Make sure you have BindIt 4.1 Software installed in your computer
- Click the protocol in the table. Protocol opens directly in BindIt Software
- Save the protocol in your computer in the preferred folder.
- Connect your PC with USB cable to KingFisher instrument and select Home > Connectin BindIt Software.
- You can start the protocol from the BindIt or transfer it into instrument. If you transfer the protocol select Home > Transfer in BindIt Software.
- Select Uploadand find the saved protocol. Click open.
- Select the folder (category) you want to store the protocol in the instrument.
- Your protocol can be now found in the instrument memory.
- Disconnect your instrument from BindIt and check using keypad that you protocol is in the desired folder.
Features of Software
- Compatible with KingFisher instruments
- Conveniently create and modify protocols on your PC and transfer to them to your KingFisher instrument. The software allows you to define and edit steps and parameters for every part of your protocol—bind, wash, elute
- Allows KingFisher plates and reagents to be defined in the plate layout
- Generates status reports that include run log, plate layout, and step parameters
Instructions for downloading and installing protocols with BindIt software:
- Make sure you have BindIt 4.1 Software installed in your computer
- Click the protocol in the table (DNA/RNA protocols, Protein protocols). Protocol opens directly in BindIt Software
- Save the protocol in your computer in the preferred folder.
- Connect your PC with USB cable to KingFisher instrument and select Home > Connect in BindIt Software.
- You can start the protocol from the BindIt or transfer it into instrument. If you transfer the protocol select Home > Transfer in BindIt Software.
- Select Upload and find the saved protocol. Click open.
- Select the folder (category) you want to store the protocol in the instrument.
- Your protocol can be now found in the instrument memory.
- Disconnect your instrument from BindIt and check using keypad that you protocol is in the desired folder.
Already have a KingFisher Instrument and BindIt Software?
BindIx for
KingFisher Apex
Features of Software
- All of the functionality of the Apex instrument on your PC
- Conveniently create and modify protocols
- Easily transfer new or edited protocols to KingFisher Apex
- Access protocol library
- Available for PC laptops or desktop computers
- Compatible with KingFisher Apex instruments only
How to install BindIx software*?
- Download BindIx here
- Download BindIx from Microsoft Store here
BindIx from Microsoft Store can be installed by selecting “Install” button on MS Store. - USB stick (delivered from manufacture)
BindIx from USB stick can be installed by double-clicking .msix bundle file and after that selecting “Install” button.
Note: System requirements for BindIx software is Windows 10 version 1809 (17763.0) or higher.
DNA & RNA extraction protocols available to download by SKU
| Nucleic acid | Sample type | Kit name | SKU | Format | Software protocol |
|---|---|---|---|---|---|
| RNA | Saliva | MagMAX Viral/Pathogen Kit (MVP I) | A48310 A42352 | up to 2,000 preps up to 200 preps | MagMAX Viral/Pathogen RNA Saliva Flex |
| MagMAX Viral/Pathogen II Kit (MVPII) | A48383 | up to 2,000 preps | |||
| DNA | Blood, buccal, swabs | MagMAX DNA Multi-Sample Ultra | A25597 A25598 | 500 preps 2,500 preps | MagMAX DNA Blood Buccal Duo Prime Flex |
| DNA | Blood, buccal, swabs | MagMAX DNA Multi-Sample Ultra w/ extra PK and beads | A25919 A25920 | 500 preps 2,500 preps | MagMAX DNA Blood Buccal Duo Prime Flex |
| DNA | Blood, buccal, swabs | MagMAX DNA Multi-Sample | 4413020 | 50 preps | MagMAX DNA Blood Buccal Duo Prime Flex |
| DNA | Blood, buccal, swabs | MagMAX-96 DNA Multi-Sample | 4413021 4413022 | 96 preps 5 x 96 preps | MagMAX DNA Blood Buccal Duo Prime Flex |
| DNA | PCR reaction | ChargeSwitch PCR Clean-Up Kit | CS12000 CS1200010 | 100 preps 960 preps | ChargeSwitch PCR reaction Flex |
| DNA | Tissue, cells | MagMAX DNA Multi-Sample Ultra | A25597 A25598 | 500 preps 2,500 preps | MagMAX DNA tissue cells Duo Prime Flex |
| DNA | Tissue, cells | MagMAX DNA Multi-Sample Ultra w/ extra PK and beads | A25919 A25920 | 500 preps 2,500 preps | MagMAX DNA tissue cells Duo Prime Flex |
| DNA | Tissue, cells | MagMAX DNA Multi-Sample | 4413021 | 50 preps | MagMAX DNA tissue cells Duo Prime Flex |
| DNA | Tissue, cells | MagMAX-96 DNA Multi-Sample | 4413021 4413022 | 96 preps 5 x 96 preps | MagMAX DNA tissue cells Duo Prime Flex |
| RNA/DNA | Viral | MagMAX Pathogen RNA/DNA | 4462359 | 480 preps | MagMAX Pathogen Std Volume Duo Prime Flex |
| RNA/DNA | Viral | MagMAX Viral RNA | AM1836 | 96 preps | MagMAX Pathogen Std Volume Duo Prime Flex |
| RNA/DNA | Viral | MagMAX Total Nucleic Acid | AM1840 | 100 preps | MagMAX Pathogen Std Volume Duo Prime Flex |
| RNA/DNA | Viral | MagMAX Pathogen RNA/DNA | 4462359 | 480 preps | MagMAX Pathogen High Volume Duo Prime Flex |
| RNA/DNA | Viral | MagMAX Viral RNA | AM1836 | 96 preps | MagMAX Pathogen High Volume Duo Prime Flex |
| RNA/DNA | Viral | MagMAX Total Nucleic Acid | AM1840 | 100 preps | MagMAX Pathogen High Volume Duo Prime Flex |
| RNA | Viral | MagMAX-96 AI/ND Viral RNA Isolation Kit | AM1835 | 384 preps | MagMAX AI/ND Viral RNA Duo Prime Flex |
| RNA/ microRNA | Tissue, cells blood, plasma, urine | MagMAX mirVana Total RNA | A27828 | 96 preps | MagMAX mirVana tissue cells Duo Prime Flex MagMAX mirVana biofluids Duo Prime Flex |
| DNA | Plasma | MagMAX Cell-Free DNA | A29319 | 96 preps | MagMAX Cell-Free DNA 2mL plasma Duo Prime Flex MagMAX Cell-Free DNA 4mL plasma Duo Prime Flex |
| RNA | Tissue, cells, plants | MagMAX-96 Total RNA | AM1830 | 96 preps | MagMAX Total RNA tissue cell plant Duo Prime Flex |
| RNA | Blood | MagMAX for Stabilized Blood (Paxgene tubes) | 4451894 | 96 preps | MagMAX for Paxgene tubes Duo Prime Flex |
| RNA | Blood | MagMAX for Stabilized Blood (Tempus tubes) | 4451893 | 96 preps | MagMAX for Tempus tubes Duo Prime Flex |
| DNA | Leukocytes | MagMAX DNA Multi-Sample Ultra | A25597 | 500 preps | MagMAX DNA leukocytes Duo Prime Flex |
| mRNA | Dynabeads mRNA Direct | 61011 61012 | 5 mL 10 mL | Dynabeads mRNA from crude samples Duo Prime Flex | |
| RNA | MagMAX for Microarrays Total RNA Isolation | AM1839 | 96 preps | MagMAX for Microarrays with 100uL aqueous phase Duo Prime Flex | |
| DNA | FFPE | MagMAX FFPE DNA/RNA Ultra Kit | A31881 | 1 kit | MagMAX FFPE Ultra DNA only Duo Prime Flex |
| RNA | FFPE | MagMAX FFPE DNA/RNA Ultra Kit | A31881 | 1 kit | MagMAX FFPE Ultra RNA only Duo Prime Flex |
| RNA/DNA | FFPE | MagMAX FFPE DNA/RNA Ultra Kit | A31881 | 1 kit | MagMAX FFPE Ultra DNA and RNA Duo Prime Flex |
Instructions for downloading and installing protocols:
- Make sure you have BindIt 4.1 Software installed in your computer
- Click the protocol in the table. Protocol opens directly in BindIt Software
- Save the protocol in your computer in the preferred folder.
- Connect your PC with USB cable to KingFisher instrument and select Home > Connectin BindIt Software.
- You can start the protocol from the BindIt or transfer it into instrument. If you transfer the protocol select Home > Transfer in BindIt Software.
- Select Uploadand find the saved protocol. Click open.
- Select the folder (category) you want to store the protocol in the instrument.
- Your protocol can be now found in the instrument memory.
- Disconnect your instrument from BindIt and check using keypad that you protocol is in the desired folder.
Immunoprecipitation protocols for KingFisher Flex or DUO Prime automation
† Strategy (Indirect or Direct). This refers to the order of binding steps, and it primarily pertains to immunoprecipitation protocols. Indirect protocols involve incubating the target-containing sample and antibody first, and then incubating with the beads second. Direct protocols involve incubating beads and antibody first (either to bind or covalently couple the IP antibody), and then incubating the bead-antibody complex with the sample.
| Ligand | Product | Protocol name: description | Strategy† | Download file |
|---|---|---|---|---|
| Protein A | DynaGreen Protein A | IP DynaGreen Protein A Direct: Immunoprecipitation (IP or Co-IP) | Direct | Flex Duo Prime |
| Protein A | DynaGreen Protein A | IP DynaGreen Protein A Indirect: Immunoprecipitation (IP or Co-IP) | Indirect | Flex Duo Prime |
| Protein A/G | DynaGreen Protein A/G | IP DynaGreen Protein A/G Direct: Immunoprecipitation (IP or Co-IP) | Direct | Flex Duo Prime |
| Protein A/G | DynaGreen Protein A/G | IP DynaGreen Protein A/G Indirect: Immunoprecipitation (IP or Co-IP) | Indirect | Flex Duo Prime |
| Capture Select | DynaGreen Capture Select | IP DynaGreen CaptureSelect Anti-IgG-Fc Direct: Immunoprecipitation (IP or Co-IP) | Direct | Flex Duo Prime |
| Capture Select | DynaGreen Capture Select | IP DynaGreen CaptureSelect Anti-IgG-Fc Indirect: Immunoprecipitation (IP or Co-IP) | Indirect | Flex Duo Prime |
| Antibodies | FabRICATOR MagIC immobilized on magnetic beads | High throughput parallel analysis of antibodies | Indirect | Flex Duo Prime |
| Protein A | Dynabeads Protein A | IP Dynabeads M-280 Protein A G Direct: Immunoprecipitation (IP or Co-IP) | Direct | Flex Duo Prime |
| Protein A | Dynabeads Protein A | IP Dynabeads M-280 Protein A G Indirect: Immunoprecipitation (IP or Co-IP) | Indirect | Flex Duo Prime |
| Protein A | Dynabeads Protein A Immunoprecipitation Kit | IP Dynabeads M-280 Protein A G Direct: Immunoprecipitation (IP or Co-IP) | Direct | Flex Duo Prime |
| Protein A | Dynabeads Protein A Immunoprecipitation Kit | IP Dynabeads M-280 Protein A G Indirect: Immunoprecipitation (IP or Co-IP) | Indirect | Flex Duo Prime |
| Protein G | Dynabeads Protein G | IP Dynabeads M-280 Protein A G Direct: Immunoprecipitation (IP or Co-IP) | Direct | Flex Duo Prime |
| Protein G | Dynabeads Protein G | IP Dynabeads M-280 Protein A G Indirect: Immunoprecipitation (IP or Co-IP) | Indirect | Flex Duo Prime |
| Protein G | Dynabeads Protein G Immunoprecipitation Kit | IP Dynabeads M-280 Protein A G Direct: Immunoprecipitation (IP or Co-IP) | Direct | Flex Duo Prime |
| Protein G | Dynabeads Protein G Immunoprecipitation Kit | IP Dynabeads M-280 Protein A G Indirect: Immunoprecipitation (IP or Co-IP) | Indirect | Flex Duo Prime |
| Sheep anti-Mouse IgG | Dynabeads Sheep anti-Mouse IgG | IP Dynabeads M-280 SAM SAR Direct: Immunoprecipitation (IP or Co-IP) | Direct | Flex Duo Prime |
| Sheep anti-Rabbit IgG | Dynabeads Sheep anti-Rabbit IgG | IP Dynabeads M-280 SAM SAR Direct: Immunoprecipitation (IP or Co-IP) | Direct | Flex Duo Prime |
| Streptavidin | Dynabeads M-280 Streptavidin | IP Dynabeads M-280 SA Direct: Immunoprecipitation (IP or Co-IP) | Direct | Flex Duo Prime |
| Glutathione | Pierce Glutathione Magnetic Agarose Beads | Purify GST tag Magnetic Agarose GSH Direct: GST fusion protein purification | Direct | Flex Duo Prime |
| Ni-IMAC | Pierce High Capacity Ni-IMAC MagBeads, EDTA-compatible | Purify His tag Magnetic Agarose Ni-IMAC Direct | Direct | Flex Duo Prime |
| Ni-NTA | Pierce Ni-NTA Magnetic Agarose Beads | Purify His tag Magnetic Agarose Ni-NTA Direct: His-tagged fusion protein purification | Direct | Flex Duo Prime |
| Protein A/G | Pierce Protein A/G Magnetic Agarose Beads | Purify Ab Magnetic Agarose Protein AG Direct: Antibody purification of IgG containing Fc region | Direct | Flex Duo Prime |
| Anti-HA Antibody | Pierce Anti-HA Magnetic Beads | IP HA tag Pierce anti-HA Ab Direct: HA-tagged fusion protein purification or immunoprecipitation (IP or Co-IP) | Direct | Flex |
| Anti-c-Myc Antibody | Pierce Anti-c-Myc Magnetic Beads | IP Myc tag Pierce anti-Myc Ab Direct: Myc-tagged fusion protein purification or immunoprecipitation (IP or Co-IP) | Direct | Flex |
| Glutathione | Pierce Glutathione Magnetic Beads | Purify GST tag Pierce GSH Direct: GST fusion protein purification or pull-down | Direct | Flex |
| Ni-NTA | HisPur Ni-NTA Magnetic Beads | Purify His tag Pierce Ni-NTA Direct: His-tagged fusion protein purification or pull-down | Direct | Flex |
| NHS Ester | Pierce NHS-Activated Magnetic Beads | Couple Pierce NHS ester Direct: Coupling (immobilization) of primary amine molecule (protein, peptide) | Direct | Flex |
| NHS Ester | Pierce NHS-Activated Magnetic Beads | IP Pierce NHS ester Direct: Affinity purification with coupled (immobilized) primary amine molecule (protein, peptide) | Direct | Flex |
| Protein A/G | Pierce Protein A/G Magnetic Beads | Purify Ab Pierce Protein AGL Direct: Antibody purification of IgG containing Fc region | Direct | Flex |
| Protein A | Pierce Protein A Magnetic Beads | Purify Ab Pierce Protein AGL Direct: Antibody purification of IgG containing Fc region | Direct | Flex |
| Protein G | Pierce Protein G Magnetic Beads | Purify Ab Pierce Protein AGL Direct: Antibody purification of IgG containing Fc region | Direct | Flex |
| Protein L | Pierce Protein L Magnetic Beads | Purify Ab Pierce Protein AGL Direct: Antibody purification of immunoglobulins containing kappa light chains | Direct | Flex |
| Protein A/G | Pierce Protein A/G Magnetic Beads | IP Pierce Protein AGL Indirect: Immunoprecipitation (IP or Co-IP) | Indirect | Flex |
| Protein A | Pierce Protein A Magnetic Beads | IP Pierce Protein AGL Indirect: Immunoprecipitation (IP or Co-IP) | Indirect | Flex |
| Protein G | Pierce Protein G Magnetic Beads | IP Pierce Protein AGL Indirect: Immunoprecipitation (IP or Co-IP) | Indirect | Flex |
| Protein A/G | Pierce Classic Magnetic IP/Co-IP Kit | IP Pierce Protein AGL Indirect: Immunoprecipitation (IP or Co-IP) | Indirect | Flex |
| NHS Ester | Pierce Direct Magnetic IP/Co-IP Kit | IP Pierce Direct IP Kit Direct: Immunoprecipitation (IP or Co-IP) or pull-down after coupling (immobilization) of IgG, protein or peptide to beads | Direct | Flex |
| Protein A/G | Pierce Crosslink Magnetic IP/Co-IP Kit | IP Pierce Crosslink IP Kit Direct: Immunoprecipitation (IP or Co-IP) after crosslinking IgG to beads | Direct | Flex |
| Protein A/G | Pierce Magnetic ChIP Kit | ChIP Pierce ChIP Kit Indirect: Chromatin immunoprecipitation (ChIP) of protein-DNA complexes | Indirect | Flex |
| Streptavidin | Pierce Streptavidin Magnetic Beads | IP Pierce SA heated elution Indirect: Purification or enrichment of biotinylated or desthiobiotinylated molecules using heated elution | Indirect | Flex |
| Streptavidin | Pierce Streptavidin Magnetic Beads | IP Pierce SA low pH elution Indirect: Purification or enrichment of biotinylated or desthiobiotinylated molecules using low-pH elution | Indirect | Flex |
| Titanium Dioxide (TiO2) | Pierce Magnetic Titanium Dioxide Phosphopeptide Enrichment Kit | Enrich pPep Pierce pPep Kit Direct deepwell plate: Phosphopeptide enrichment in deep-well plates (peptides containing phosphorylated amino acids, e.g., phosphoserine, phosphotyrosine or phosphotyrosine) | Direct | Flex |
| Titanium Dioxide (TiO2) | Pierce Magnetic Titanium Dioxide Phosphopeptide Enrichment Kit | Enrich pPep Pierce pPep Kit Direct PCR plate: Phosphopeptide enrichment in PCR plates (peptides containing phosphorylated amino acids, e.g., phosphoserine, phosphotyrosine or phosphotyrosine) | Direct | Flex |
Instructions for downloading and installing protocols:
- Make sure you have BindIt 4.1 Software installed in your computer
- Click the protocol in the table. Protocol opens directly in BindIt Software
- Save the protocol in your computer in the preferred folder.
- Connect your PC with USB cable to KingFisher instrument and select Home > Connect in BindIt Software.
- You can start the protocol from the BindIt or transfer it into instrument. If you transfer the protocol select Home > Transfer in BindIt Software.
- Select Upload and find the saved protocol. Click open.
- Select the folder (category) you want to store the protocol in the instrument.
- Your protocol can be now found in the instrument memory.
- Disconnect your instrument from BindIt and check using keypad that you protocol is in the desired folder.
Dynabeads virus enrichment and positive cell isolation protocols to download
| Ligand | Product | Protocol description | Strategy | Download file |
|---|---|---|---|---|
| Virus | Viruses (especially SARS-CoV-2), vesicles and proteins | Dynabeads Intact Virus Enrichment | Indirect | Duo Prime |
| Virus | Viruses (especially SARS-CoV-2), vesicles and proteins | Dynabeads Intact Virus Enrichment | Indirect | Flex |
| Cells | CD3, CD4, CD8, CD14, CD19 | Positive Cell Isolation for downstream nucleic acid, protein analysis, or cell depletion | Indirect | Duo Prime |
| Cells | CD3, CD4, CD8 | Positive Cell Isolation for downstream cellular applications – such as T-cell Activation | Indirect | Duo Prime |
Instructions for downloading and installing protocols:
- Make sure you have BindIt 4.1 Software installed in your computer
- Click the protocol in the table. Protocol opens directly in BindIt Software
- Save the protocol in your computer in the preferred folder.
- Connect your PC with USB cable to KingFisher instrument and select Home > Connectin BindIt Software.
- You can start the protocol from the BindIt or transfer it into instrument. If you transfer the protocol select Home > Transfer in BindIt Software.
- Select Uploadand find the saved protocol. Click open.
- Select the folder (category) you want to store the protocol in the instrument.
- Your protocol can be now found in the instrument memory.
- Disconnect your instrument from BindIt and check using keypad that you protocol is in the desired folder.
For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.