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Learn how to leverage the latest technologies available from Life Technologies with these useful technical resources for epigenetics and noncoding RNA research.
Application Notes and Technical Guides |
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High Resolution Methylation (HRM) Analysis |
Methylation Analysis Using Methylation-Sensitive HRM and DNA Sequencing This application note discusses studying DNA methylation with HRM, followed by DNA sequencing. |
Getting Started with Bisulfite Primer Design |
CE Bisulfite Sequencing Guide |
DNA Methylation Enrichment Deep Sequencing Using NGS Technology |
Posters |
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DNA Methylome Sequencing: Methylated DNA Enrichment with Next-Generation Sequencing by Ligation |
Direct Bisulfite Conversion from Archived Tumor Samples for Methylation Detection |
Application Notes and Technical Guides |
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Noncoding RNA Characterization with TaqMan® ncRNA Assay This application note discusses how to leverage TaqMan® ncRNA Assays to better understand the role of larger, noncoding RNAs in gene regulation. |
miRNA Profiling Experimental Planning Get advice on sample size for experiments, review data analysis methods and tools, and review successful microRNA (miRNA) profiling experiments to learn more about any special equipment necessary. |
Choosing Endogenous Controls for Real-Time Quantitation |
Databases and Software |
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NCode™ Profiler Software An advanced experimental design and analysis solution designed for two-dye expression profiling microarray experiments. The software minimizes inherent challenges with miRNA array design and data analysis. |
Webinars |
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miRNA Expression in Cancer |
miRNA in Human Embryonic Stem Cell (hESC) Differentiation Life Technologies scientist Mark Landers reviews the role miRNA appears to play in hESC differentiating and effective approaches for profiling miRNA in these samples. |
Posters |
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microRNA Quantitation by Real-Time PCR |
TaqMan® Assay-Based miRNA Profiles Classify Embryonic Stem and Differentiated Cells Researchers used a large panel of TaqMan® MicroRNA Assays to quantify miRNA expression levels in four mouse embryonic stem cell lines and their differentiated embryoid bodies, along with six normal mouse tissues. |
High Correlation of miRNA Quantitation Data from Matched FFPE and Snap-Frozen Tissues using TaqMan® MicroRNA Assays This application note describes the successful isolation and detection of miRNAs from formalin-fixed, paraffin-embedded (FFPE) samples and demonstrates the accurate and robust measurement of miRNA expression from comparable snap-frozen samples using TaqMan® MicroRNA Assays. |
miRNA Expression Profiling in Brain Tumor by Real-Time PCR A significant difference in miRNA expression was evident between normal and GM tumor samples. |
Our Learning Center provides an overview of epigenetic regulation of gene expression as well as considerations underpinning the research workflow. Learn about: