TaqMan Probes and qPCR Primers

TaqMan probes and qPCR primers are an optimal choice for real-time PCR research experiments that cannot be addressed with Thermo Fisher Scientific predesigned, ready-to-use TaqMan assays. Researchers can select from a collection of dual-labeled TaqMan probes and unlabeled oligos for qPCR primers, providing the flexibility to meet various experimental needs. Whether researchers target multiple sequences simultaneously, using custom probes via bioinformatics, or detecting exotic targets that do not already have a predesigned assay, Thermo Fisher Scientific offers excellent probes for designing custom assays. The TaqMan MGB (minor groove binder), TAMRA, QSY, and QSY2 custom probes deliver exceptional performance. Manufactured with the same facilities and raw materials as the TaqMan assays featured in over 296,000 publications, these custom qPCR probes and primers help ensure quality and reliability.

TaqMan probes are highly reliable and widely used in various research applications, particularly in quantitative PCR (qPCR) experiments. These customizable probes are designed to specifically bind to the target sequence, allowing for precise and sensitive detection of the desired nucleic acid. TaqMan probes utilize a dual-labeled format, with a fluorophore at the 5' end and a quencher at the 3' end. During amplification, the probe is cleaved by the DNA polymerase, resulting in the separation of the fluorophore and quencher, leading to a fluorescent signal. This signal is directly proportional to the amount of target nucleic acid present in the sample. TaqMan probes are known for their exceptional quality, reproducibility, and widely used chemistry, making them a trusted choice for researchers seeking accurate and reliable results in their qPCR experiments.

Design and order custom TaqMan qPCR probes

qPCR primers are essential tools in the field of molecular biology, designed to amplify specific DNA sequences with high precision and efficiency. Sequence detection primers are custom primers for real-time PCR that can be used with either TaqMan probes or SYBR dye, making them versatile for real-time PCR research applications. Tailor-made to match the unique requirements of each experimental setup, these custom primers help ensure accurate target amplification, thereby enhancing the sensitivity and specificity of the assay for more reliable and reproducible data. Additionally, these qPCR primers are desalted, available in various scales, and can be shipped either as liquid or dried down, providing flexibility and convenience for researchers. Custom qPCR primers allow for optimal performance in diverse applications, including gene expression analysis, genotyping, and pathogen detection.

Design and order custom qPCR primers

• HPLC-purified probes—Removing impurities such as unconjugated dyes or truncated probes that can increase background signal and decrease assay sensitivity 
• Option to ship in solution—Avoid errors during resuspension that can alter stock concentrations and markedly affect downstream experiments 
• Guaranteed yield—Ensures the right amount of qPCR assays for every sample size and scale 
• Efficient integration—TaqMan probes are designed for seamless integration into the Applied Biosystems workflow, including latest master mixes 
• Competitive shipping—Similar turnaround times in North America as compared to competitors*

*Estimated 4–10 days in North America. International times may vary.