TaqMan Lyo-Ready qPCR Master Mixes | Thermo Fisher Scientific

Reliable and storage-temperature-agnostic molecular diagnostics qPCR assays need to utilize reagents that are easily lyophilized and provide consistent performance even with non-ideal samples. TaqMan Lyo-Ready qPCR master mixes fulfill these requirements, making them highly suited to lyophilized qPCR assays using your chosen primers and probes.

TaqMan Lyo-Ready qPCR master mixes are designed for consistent performance even in demanding applications, including virus and bacteria detection. Easy to lyophilize and fast to re-hydrate, they ensure consistency before and after lyophilization even in multiplex reactions.

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TaqMan Lyo-Ready qPCR master mixes are available in several formulations. Customization is also available and can be requested by contacting your Thermo Fisher Scientific representative or emailing custom.solutions@thermofisher.com.

Which TaqMan Lyo-Ready master mix is right for your application?

	TaqMan 2.5X Lyo-Ready 1-Step Master Mix with excipient	TaqMan 2.5X Lyo-Ready qPCR Master Mix with excipient	TaqMan 5X Lyo-Ready 1-Step Master Mix	TaqMan 5X Lyo-Ready 1-Step Master Mix with ROX	TaqMan 5X Lyo-Ready qPCR Master Mix	TaqMan 5X Lyo-Ready qPCR Master Mix with ROX
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Target	RNA, DNA	DNA	RNA, DNA	RNA, DNA	DNA	DNA
Concentration	2.5X	2.5X	5X	5X	5X	5X
Excipient	Yes, pre-mixed	Yes, pre-mixed	None	None	None	None
Passive reference dye	None	None	None	ROX	None	ROX
Reverse transcriptase	Thermostable M-MLV	N/A	Thermostable M-MLV	Thermostable M-MLV	N/A	N/A
Pre-PCR benchtop stability	4 hr*	4 hr*	<2 hr*	<2 hr*	<2 hr*	<2 hr*
Multiplexing targets	5	5	5	3	5	3
Sample SKU	C14031SMP (1.5 mL)	C14081SMP (1.5 mL)	C1403SMP (1.5 mL)	C1402SMP (1.5 mL)	C1408SMP (1.5 mL)	C1407SMP (1.5 mL)
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This product is best for you if you are	New to lyophilization Would like to develop and manufacture a lyophilized qPCR assay Need ready-to-use reagents and comprehensive guidance on the process		Experiencedin lyophilization Have your own excipient and lyophilization program Need a qPCR mix optimized for consistent and reliable performance before and after lyophilization

*Recommend setting up plate on ice and running immediately. Stability is assay dependent. Greater stability has been observed for some assays.

Technical Literatures

Brochure: TaqMan 2.5X Lyo-Ready qPCR Master Mix With Excipient

Brochure: TaqMan 2.5X Lyo-Ready 1-Step qPCR Master Mix With Excipient

Whitepaper: Switch easily from liquid to lyophillized format using TaqMan Lyo -ready master mix with excipient

TaqMan Lyo-Ready qPCR master mixes are reagents used in further manufacturing (lyophilization) and labeled “Caution: For use as a raw material in further manufacturing applications”, manufactured in an ISO 13485 certified and FDA registered facility, and optimized for exceptional lot-to-lot consistency, inhibitor tolerance, and multiplexing.