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Surface-Activated Dynabeads |
Dynabeads magnetic beads are available for covalent coupling of ligands to the bead surface. One can choose beads of different sizes, with a hydrophobic or hydrophilic surface, and with a variety of surface chemistries. For optimal results, select the right bead based on ligand type (Table 1). Note that in most applications several Dynabeads products can be used. Depending on the magnetic bead type, the ligand can be directly linked to the bead surface or indirectly linked through a reactive intermediate. Protocols can take place in a single tube, with just a few handling steps.
The Dynabeads are pipetted into a vial and the ligand is added. Incubate the vial for covalent coupling of the ligand to the Dynabeads, add target containing sample, incubate again for affinity-binding of target. Use a magnetic stand or rack for magnetic separation, and remove supernatant. Wash and either boil in SDS for page or elute for pure target protein.
Figure 1. Antibodies or other ligands can be coupled onto the beads for affinity protein capture.
Ligand | Target | Dynabeads Tosyl- activated | Dynabeads Carboxylic Acid | Dynabeads Amine | Dynabeads Epoxy |
---|---|---|---|---|---|
Antibody | Low MW antigen1 or peptide | ![]() ![]() ![]() | ![]() ![]() | ![]() ![]() | ![]() ![]() |
Protein or antibody | ![]() ![]() ![]() | ![]() ![]() | ![]() | ![]() ![]() | |
Protein complex | ![]() ![]() | ![]() | ![]() | ![]() ![]() ![]() | |
Organelles | ![]() ![]() | ![]() ![]() | |||
Phage1 | ![]() ![]() ![]() | ![]() ![]() | ![]() ![]() | ![]() ![]() | |
Virus | ![]() ![]() ![]() | ![]() ![]() | ![]() ![]() | ![]() ![]() | |
Bacteria | ![]() ![]() ![]() | ![]() ![]() | |||
Cells3 | ![]() ![]() ![]() | ![]() ![]() | ![]() ![]() ![]() | ||
Antibody Fragment | Phage1 or antibody | ![]() ![]() | ![]() ![]() ![]() | ![]() ![]() ![]() | ![]() ![]() |
Protein | Phage or carbohydrate | ![]() ![]() | ![]() ![]() | ![]() ![]() | ![]() ![]() ![]() |
Nucleic acid | ![]() | ![]() ![]() ![]() | ![]() | ![]() | |
Peptide | Phage1 or antibody | ![]() ![]() | ![]() ![]() ![]() | ![]() ![]() ![]() | ![]() ![]() |
Carbohydrate | Antibody | ![]() | ![]() ![]() ![]() | ![]() | |
Low MV antigen | Antibody | ![]() ![]() | ![]() ![]() | ![]() ![]() | ![]() ![]() |
Nucleic acids, oligonucleotide, aptamer, PNA | Nucleic acid binding proteins1 | ![]() | ![]() ![]() | ![]() | |
DNA1, RNA1 or PCR amplicons1 | ![]() ![]() ![]() | ![]() | |||
Enzyme | Substrate or target for enzyme degradation | ![]() ![]() | ![]() | ![]() | ![]() ![]() ![]() |
Organic chemistry derivatization, including introduction of new functional groups | ![]() ![]() | ![]() ![]() | ![]() ![]() ![]() | ![]() ![]() |
Best product choice for listed ligand and target.
Alternative choice for listed ligand and target.
Can be used for listed ligand and target.
1 Dynabeads pre-coupled with streptavidin may be the best product choice.
2 Dynabeads M-450 Epoxy.
3 Dynabeads M-450 Epoxy are recommended for isolation, stimulation, and expansion of cells.
4 Bifunctional crosslinkers may be introduced to immobilize oligonucleotides onto the surface of Dynabeads Amine.
For Research Use Only. Not for use in diagnostic procedures.