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Depletion is used to reduce the complexity of biological samples such as serum, plasma, or biofluids, which contain high concentrations of albumin and immunoglobulins. These products utilize immunoaffinity techniques to enable the removal of the most abundant proteins, thus enhancing the detection of lower-abundance proteins in both discovery and targeted proteomic analyses.
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Pierce Albumin Depletion Kit | High-Select HSA/Immunoglobulin Depletion Spin Columns | High-Select Top14 Abundant Protein Depletion Spin Columns | |
Proteins depleted | Albumin | Albumin, IgG, IgG (light chains), IgA, IgM, IgD, IgE | 14 abundant proteins* |
Sample volume capacity | 10–50 µL | 10 μL (mini) or 100 μL (midi) | 10 μL (mini) or 100 μL (midi) |
Processing time | 20–30 min | 5–10 min | 5–10 min |
Format | Loose resin, spin columns, buffers | Pre-filled mini or midi spin columns, bulk resin | Pre-filled mini or midi spin columns, bulk resin |
*Top 14 proteins include Albumin, IgG, IgG (light chains), IgA, IgM, IgD, IgE, α1-Acid glycoprotein, Fibrinogen, Haptoglobin, α1-antitrypsin, α2-macroglobulin, Transferrin, Apolipoprotein A-I
Figure 3. Abundant protein depletion improves identification of unique proteins. Protein group identification profiles for normal human plasma samples which were (a) not depleted, or depleted using the HSA/Immunoglobulin (b) and Top14 (c) depletion resins, are shown. All samples were reduced/alkylated and digested with trypsin. Samples were analyzed by LC-MS on an Orbitrap Fusion Tribrid mass spectrometer over a 60 minute gradient using 750 ng of sample per injection (performed in triplicate). Raw files were searched against a human protein database using Thermo Scientific Proteome Discoverer 1.4 software. Non-redundant protein group identification numbers are reported for each sample type.
Figure 3. Abundant protein depletion improves identification of unique proteins. Protein group identification profiles for normal human plasma samples which were (a) not depleted, or depleted using the HSA/Immunoglobulin (b) and Top14 (c) depletion resins, are shown. All samples were reduced/alkylated and digested with trypsin. Samples were analyzed by LC-MS on an Orbitrap Fusion Tribrid mass spectrometer over a 60 minute gradient using 750 ng of sample per injection (performed in triplicate). Raw files were searched against a human protein database using Thermo Scientific Proteome Discoverer 1.4 software. Non-redundant protein group identification numbers are reported for each sample type.
For Research Use Only. Not for use in diagnostic procedures.