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Membrane Protein Extraction and Isolation
Our membrane protein isolation reagents, kits, and detergents are optimized for membrane protein isolation and enrichment, enable high protein yield, preserve protein function, and have been optimized for minimum contamination. The membrane proteins that are isolated with these assays are compatible with other protein assays and downstream applications.
Membrane protein detergents
| Detergent | Type | MW | CMC% w/v (mM) | Dialyzable | Compatible with Detergent Removal Resin |
|---|---|---|---|---|---|
| n-Dodecyl-β-Maltoside Detergent (DDM) | Nonionic | 510 | 0.009% (0.17) | No | Yes |
| Lauryl Maltose Neopentyl Glycol (LMNG) | Nonionic | 1005.19 | ∼0.001%, (0.01) | Yes | No |
| n-Undecyl-β-D-maltopyranoside (UDM) | Nonionic | 496.6 | 0.029% (0.58) | Yes | Yes |
| n-Octyl-β-D-Maltopyranoside (OM) | Nonionic | 454.4 | 0.89% (19.5) in 100 mM NaCl, 20 mM HEPES pH 7.5 | Yes | No |
| n-Nonyl-β-Maltoside (NM) | Nonionic | 468.5 | 0.28% (6) | Yes | Yes |
| n-Nonyl-β-D-glucoside (NG) | Nonionic | 306.4 | 0.20% (6.5) | Yes | Yes |
| Octyl-β-Glucoside Detergent (OG) | Nonionic | 292 | 0.6716–0.7300% (23–25) | Yes | Yes |
| Octylthioglucoside (OTG) | Nonionic | 308 | 0.2772% (9) | Yes | Yes |
| 5-Cyclohexyl-1-Pentyl-β-D-Maltoside | Nonionic | 494.5 | 0.12% (2.4–5.0) | Yes | Yes |
| 6-Cyclohexyl-1-Hexyl-β-D-Maltoside | Nonionic | 508.5 | 0.028% (~0.56) | Yes | Yes |
| 7-Cyclohexyl-1-Heptyl-β-D-Maltoside | Nonionic | 522.5 | 0.0099% (~0.19) | Yes | Yes |
| Tetraethylene Glycol Monooctyl Ether (C8E4) | Nonionic | 306.5 | 0.25% (~8) | Yes | Yes |
| CHAPS | Zwitterionic | 615 | 0.4920–0.6150% (8–10) | Yes | Yes |
| CHAPSO | Zwitterionic | 630.9 | 0.50% (8) | Yes | No |
| Lauryldimethylamine-N-oxide (LDAO) | Zwitterionic | 229.4 | 0.023% (~1–2) | Yes | Yes |
| Fluorinated Fos-Choline-8 | Zwitterionic | 529.2 | ~2.9 | Yes | Yes |
| Fos-choline-12 | Zwitterionic | 351.5 | 0.047% (1.5) | Yes | Yes |
| Fos-choline-15 | Zwitterionic | 393.5 | 0.0027% (0.07) | Yes | No |
Membrane protein isolation kits
| GPCR Extraction and Stabilization Reagent | Mem-PER Plus Membrane Protein Extraction Kit | Cell Surface Protein Biotinylation and Isolation Kit | |
|---|---|---|---|
| Membrane protein enrichment | G Protein-Coupled Receptors (GPCR) | Plasma membrane proteins | Integral and membrane associated proteins |
| Protein functionality | Maintains structural integrity as measured by immunoprecipitation and receptor ligand binding assays | Enrichment compatible with western blotting | Enrichment compatible with western blotting and MS applications |
| Compatible sample types | Tissues and cultured mammalian cells | Tissues and cultured mammalian cells | Cultured mammalian cells |
| Cytosolic contamination | Whole cell lysate | Less than 5% contamination of cytosolic proteins | Less than 20% contamination of cytosolic proteins |
| Sample processing time | 1–2 hours | 1 hour | 2 hours |
| Mechanical disruption required? | Yes, for tissue | Yes, for tissue | No |
| Amount of sample processed | 100 samples with 107 cells or 100 samples with 50–100 mg tissue | 50 samples with 5 million cells or 25 samples of 20–40 mg tissue per kit | 8 experiments, with two confluent 15 cm dishes or four T75 flasks |
| Protein assay compatibility | BCA Protein Assays | BCA Protein Assays, detergent-compatible Bradford | Lysis buffer is compatible with BCA and Rapid Gold BCA; elution buffer is not compatible. |
| Downstream compatibility | Western blot, IP, protein purification, radio-ligand binding assays | IP, western blot, ELISA, amine reactive labeling | Western blot, ELISA, mass spectrometry analysis |
| Protease or phosphatase inhibitors recommended? | Yes, both | Yes, both | Yes, both |
| Cat. No. | A43436 | 89842 | A44390 |
Reagent-based membrane protein extraction and membrane protein isolation
Reagent-based lysis has replaced traditional physical lysis as the method of choice for membrane disruption and extraction of membrane proteins. Reagent-based lysis methods do not require expensive, cumbersome equipment and protocols that are difficult to implement. The Thermo Scientific membrane protein extraction and membrane protein isolation reagents consist of optimized concentrations of detergents, buffers, salts, and reducing agents developed for particular species and types of cells. Reagents also have the added benefits of both lysing and solubilizing effects. Our membrane protein isolation and membrane protein extraction kits are optimized specifically for your needs to isolate high-quality protein samples essential to completing successful downstream applications.
Features:
- Extraction and isolation—produces minimal cross-contamination (typically less than 10%) of cytosolic protein into the membrane protein fraction
- Cells or tissues—effective for extraction from cultured mammalian cells and mammalian tissues
- Downstream compatibility—analyze membrane protein extracts by SDS-PAGE, western blotting, immunoprecipitation, and protein assays
- Fast and simple—isolation of membrane proteins in approximately one hour
- No special equipment required—only a benchtop microcentrifuge, tubes, homogenizers, and pipettors are needed
Featured webinar
Strategies for isolation of plasma membrane proteins
This webinar will focus on robust and optimized techniques for extraction, isolation and enrichment of cell surface proteins, including stable and functional G protein-coupled receptors (GPCRs).
Product manuals
Application notes
Membrane protein detergents
| Detergent | Type | MW | CMC% w/v (mM) | Dialyzable | Compatible with Detergent Removal Resin |
|---|---|---|---|---|---|
| n-Dodecyl-β-Maltoside Detergent (DDM) | Nonionic | 510 | 0.009% (0.17) | No | Yes |
| Lauryl Maltose Neopentyl Glycol (LMNG) | Nonionic | 1005.19 | ∼0.001%, (0.01) | Yes | No |
| n-Undecyl-β-D-maltopyranoside (UDM) | Nonionic | 496.6 | 0.029% (0.58) | Yes | Yes |
| n-Octyl-β-D-Maltopyranoside (OM) | Nonionic | 454.4 | 0.89% (19.5) in 100 mM NaCl, 20 mM HEPES pH 7.5 | Yes | No |
| n-Nonyl-β-Maltoside (NM) | Nonionic | 468.5 | 0.28% (6) | Yes | Yes |
| n-Nonyl-β-D-glucoside (NG) | Nonionic | 306.4 | 0.20% (6.5) | Yes | Yes |
| Octyl-β-Glucoside Detergent (OG) | Nonionic | 292 | 0.6716–0.7300% (23–25) | Yes | Yes |
| Octylthioglucoside (OTG) | Nonionic | 308 | 0.2772% (9) | Yes | Yes |
| 5-Cyclohexyl-1-Pentyl-β-D-Maltoside | Nonionic | 494.5 | 0.12% (2.4–5.0) | Yes | Yes |
| 6-Cyclohexyl-1-Hexyl-β-D-Maltoside | Nonionic | 508.5 | 0.028% (~0.56) | Yes | Yes |
| 7-Cyclohexyl-1-Heptyl-β-D-Maltoside | Nonionic | 522.5 | 0.0099% (~0.19) | Yes | Yes |
| Tetraethylene Glycol Monooctyl Ether (C8E4) | Nonionic | 306.5 | 0.25% (~8) | Yes | Yes |
| CHAPS | Zwitterionic | 615 | 0.4920–0.6150% (8–10) | Yes | Yes |
| CHAPSO | Zwitterionic | 630.9 | 0.50% (8) | Yes | No |
| Lauryldimethylamine-N-oxide (LDAO) | Zwitterionic | 229.4 | 0.023% (~1–2) | Yes | Yes |
| Fluorinated Fos-Choline-8 | Zwitterionic | 529.2 | ~2.9 | Yes | Yes |
| Fos-choline-12 | Zwitterionic | 351.5 | 0.047% (1.5) | Yes | Yes |
| Fos-choline-15 | Zwitterionic | 393.5 | 0.0027% (0.07) | Yes | No |
Membrane protein isolation kits
| GPCR Extraction and Stabilization Reagent | Mem-PER Plus Membrane Protein Extraction Kit | Cell Surface Protein Biotinylation and Isolation Kit | |
|---|---|---|---|
| Membrane protein enrichment | G Protein-Coupled Receptors (GPCR) | Plasma membrane proteins | Integral and membrane associated proteins |
| Protein functionality | Maintains structural integrity as measured by immunoprecipitation and receptor ligand binding assays | Enrichment compatible with western blotting | Enrichment compatible with western blotting and MS applications |
| Compatible sample types | Tissues and cultured mammalian cells | Tissues and cultured mammalian cells | Cultured mammalian cells |
| Cytosolic contamination | Whole cell lysate | Less than 5% contamination of cytosolic proteins | Less than 20% contamination of cytosolic proteins |
| Sample processing time | 1–2 hours | 1 hour | 2 hours |
| Mechanical disruption required? | Yes, for tissue | Yes, for tissue | No |
| Amount of sample processed | 100 samples with 107 cells or 100 samples with 50–100 mg tissue | 50 samples with 5 million cells or 25 samples of 20–40 mg tissue per kit | 8 experiments, with two confluent 15 cm dishes or four T75 flasks |
| Protein assay compatibility | BCA Protein Assays | BCA Protein Assays, detergent-compatible Bradford | Lysis buffer is compatible with BCA and Rapid Gold BCA; elution buffer is not compatible. |
| Downstream compatibility | Western blot, IP, protein purification, radio-ligand binding assays | IP, western blot, ELISA, amine reactive labeling | Western blot, ELISA, mass spectrometry analysis |
| Protease or phosphatase inhibitors recommended? | Yes, both | Yes, both | Yes, both |
| Cat. No. | A43436 | 89842 | A44390 |
Reagent-based membrane protein extraction and membrane protein isolation
Reagent-based lysis has replaced traditional physical lysis as the method of choice for membrane disruption and extraction of membrane proteins. Reagent-based lysis methods do not require expensive, cumbersome equipment and protocols that are difficult to implement. The Thermo Scientific membrane protein extraction and membrane protein isolation reagents consist of optimized concentrations of detergents, buffers, salts, and reducing agents developed for particular species and types of cells. Reagents also have the added benefits of both lysing and solubilizing effects. Our membrane protein isolation and membrane protein extraction kits are optimized specifically for your needs to isolate high-quality protein samples essential to completing successful downstream applications.
Features:
- Extraction and isolation—produces minimal cross-contamination (typically less than 10%) of cytosolic protein into the membrane protein fraction
- Cells or tissues—effective for extraction from cultured mammalian cells and mammalian tissues
- Downstream compatibility—analyze membrane protein extracts by SDS-PAGE, western blotting, immunoprecipitation, and protein assays
- Fast and simple—isolation of membrane proteins in approximately one hour
- No special equipment required—only a benchtop microcentrifuge, tubes, homogenizers, and pipettors are needed
Featured webinar
Strategies for isolation of plasma membrane proteins
This webinar will focus on robust and optimized techniques for extraction, isolation and enrichment of cell surface proteins, including stable and functional G protein-coupled receptors (GPCRs).
Product manuals
Application notes
Related products and downstream applications
For Research Use Only. Not for use in diagnostic procedures.