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Protein extraction, purification, and cleanup methods frequently produce dilute samples of target biomolecules in buffer matrices incompatible with downstream applications. Pierce protein concentrators are disposable ultrafiltration centrifugal devices used to concentrate, desalt, and buffer exchange biological samples, such as proteins and nucleic acids, readying them for downstream applications with minimal sample handling. Pierce protein concentrators are available in four sizes (0.5, 6, 20, and 100 mL) and offer pH compatibility over a broad pH range (1-9).
For larger volume sizes, Pierce crossflow filtration cassettes are designed for high purity and recovery of peptides, proteins, and virus/viral particles. They are used to concentrate large supernatant volumes (0.5 L–6 L). Additional accessories are available for use with the crossflow filtration cassettes.
Protein concentration | NEW Crossflow filtration cassettes | ||||
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Sample volume | 0.1 mL–0.5 mL | 2 mL–6 mL | 5 mL–20 mL | 20 mL–100 mL | 0.5 L–6 L |
See all Protein Concentrators, 3K or 5K MWCO * | See all Crossflow Filtration Cassettes, 3-100 kDa MWCO | ||||
See all Protein Concentrators, 10K MWCO | |||||
See all Protein Concentrators, 30K MWCO | |||||
See all Protein Concentrators, 50K MWCO | |||||
See all Protein Concentrators, 100K MWCO |
*MWCO = molecular weight cutoff
0.1–0.5 mL concentrators
Watch how to concentrate protein solution for sample volumes up to 0.5 mL using the microcentrifuge tube format. Membranes are available with 3K, 10K, 30K, 50K, and 100K MWCO.
2–6 mL concentrators
Watch how to concentrate protein solution for sample volumes up to 6 mL using the 15 mL centrifuge tube format. Membranes are available with 3K, 10K, 30K, 50K, or 100K MWCO.
5–20 mL concentrators
Watch how to concentrate protein solution for sample volumes up to 20 mL using the 20 mL tube format. Membranes are available with 3K, 10K, 30K, 50K, and 100K MWCO.
20–100 mL concentrators
Watch how to concentrate protein solution for sample volumes up to 100 mL using the 250 mL centrifuge bottle format. Membranes are available with 5K, 10K, 30K, 50K, and 100K MWCO.
0.5–6 L concentrators
Watch how to concentrate protein solution for sample volumes up to 6 liters using the Crossflow Filtration Cassette. These tangential flow filtration (TFF) cassettes are available in 3K, 10K, 30K, 50K, and 100K MWCO.
For Research Use Only. Not for use in diagnostic procedures.