Protein Enrichment | Thermo Fisher Scientific

Numerous techniques are available for the isolation of cellular proteins based on their post-translational modification (PTM) or active binding site. Protein enrichment is essential for isolating low-abundant proteins or isolating protein classes. Enrichment of specific proteins or protein complexes can most easily be accomplished using target-specific immobilized antibody or PTM specific affinity binding. The novel Thermo Scientific™ ActivX™ probes and enrichment kits enable the specific targeting of several enzyme classes, including kinases, GTPases, and serine hydrolases.

Comprehensive—reagents and kits for multiple protein enrichment strategies
Optimized—all reagents and kits are designed to maximize protein yield and reduce nonspecific binding
Convenient—easy-to-use spin formats enable more rapid enrichment of proteins
Flexible—kits can be used with either cultured mammalian cells or tissues

Choose the right protein enrichment kit for your application


	Phospho- protein Enrichment Kit	Glycoprotein Enrichment Kit, ConA	Glycoprotein Enrichment Kit, WGA	Ubiquitin Enrichment Kit	Kinase Enrichment Kit with ATP Probe	ActivX™ TAMRA-FP Serine Hydrolase Probe
Target	Phosphorylated proteins	α-linked mannose and terminal glucose residues	N-acetyl glucosamine (GlcNAc)	Polyubiquitin-ated proteins	ATP binding sites	Active serine hydrolase enzymes
Binding/ labeling mechanism	Metal chelate affinity to phosphate groups	Lectin affinity to sugars	Lectin affinity to sugars	Antibody affinity to target	Biotinylated ATP analog to active-site lysine	TAMRA-labeled fluoro phosphonate binds to active site serine
Processing time	2 hr	<1 hr	<1 hr	<45 min	6.5 hr	3 hr
Enrichment format	IMAC-agarose	Immobilized conA-lectin-agarose	Immobilized WGA-lectin-agarose	Immobilized polyubiquitin affinity-agarose	Immobilized strepavidin-agarose	Immobilized anti-TAMRA mAb-agarose
	90003 (10 columns)	89804 (10 rxn)	89805 (10 rxn)	89899 (15 rxn)	88310 (16 rxn)	88318 (6.8 µg)

For downstream mass spectrometry analysis, peptide enrichment and/or fractionation is often necessary to detect peptides from low-abundance proteins and/or for reducing sample complexity for proteomic analysis.

Featured product data

Faster and higher phosphoprotein yields with the Pierce Phosphoprotein Enrichment Kit.

Kit	Phosphoprotein yield (ug)*	Enrichment time (hours)
Pierce Phosphoprotein Enrichment Kit	300	1.5
Supplier Q kit	88	4.5
Supplier I kit	52**	3.5
Supplier C kit	160	3
Supplier E kit	Too dilute to determine	5

*From 2 mg total protein.
**Based on maximum 1 mg load per manufacturer’s protocol.

Resources

For Research Use Only. Not for use in diagnostic procedures.