Recombinant Fusion Protein Purification, displaying HisPur cobalt resin

We offer a variety of products, such as magnetic beads and resins, for the purification of recombinant proteins from E. coli, yeast, insect, or mammalian cell cultures. Magnetic beads and resin have high mg-scale protein binding capacity and are available in multiple formats to accommodate a variety of needs (high-throughput screening, batch, pilot, and process purification).

  • Broad product selection—choose affinity supports for the purification of proteins with a variety of fusion tags, including 6xHis, GST, DYKDDDDK (FLAG), c-myc, and HA.
  • More formats—magnetic beads, magnetic agarose, loose resin, spin columns and kits, FPLC cartridges, and 96-well filter plates enable fusion protein purification from microgram to low kilogram scales.
  • High performance—resins are designed to maximize protein yield and reduce background.
  • Economical—pricing is similar to or better than other suppliers.
     

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Choose a resin based on recombinant or epitope tag

 His-tagged (6xHis)
proteins		GST-tagged
proteins		Other epitope-tagged proteins
Ligands availableNickel, cobaltGlutathioneAnti-DYKDDDDK (FLAG),
Anti-c-Myc antibody,
or Anti-HA antibody
Formatsmagnetic beads, filter plates, loose resins (magnetic agarose, standard agarose and superflow agarose), spin columns and kits, FPLC cartridgesmagnetic beads, filter plates, loose resins (magnetic agarose, standard agarose, and superflow agarose), spin columns and kits, FPLC cartridgesmagnetic beads, loose resin (agarose, UltraLink)
Processing scaleµg–kgµg–kgµg–g
Select productsHis-tagged Protein PurificationGST-tagged Protein PurificationEpitope-tagged Protein Purification

Example product data
High-yield, high-purity, medium-scale purification of 6xHis-Tagged protein with HisPur Ni-NTA Superflow Agarose

High-yield, high-purity, medium-scale purification of 6xHis-Tagged protein. More than 4 grams of over-expressed 6xHis-GFP were purified in 3 hours using 200 mL columns containing HisPur Ni-NTA Superflow Agarose or Qiagen Ni-NTA Superflow. One liter of lysate was loaded at a flow rate of 20 mL/min, then washed until baseline with wash buffer containing 30 mM imidazole. Bound protein was eluted with buffer containing 300 mM imidazole. Fractions containing purified 6xHis-GFP were pooled and quantitated using Pierce 660 nm Protein Assay (Cat. No. 22662). Load, flow-through, wash, and eluate fractions were separated by SDS-PAGE, stained with Imperial Protein Stain (Cat. No. 24615) and evaluated using myImageAnalysis Software (Cat. No. 62237) to determine purity.

See experiment details and additional data

Thermo Scientific Pierce Glutathione Agarose facilitates high yield and high purity GST-fusion protein purification.E. coli lysate (14.4 mg total protein) containing overexpressed GST was incubated with 50 µL GSH resin from various suppliers and purified per manufacturers' instructions. The amount of GST eluted from the resin (yield) was quantified by Thermo Scientific Coomassie Plus Protein Assay. Purity was assessed by densitometry of the stained gel lanes. M= MW marker; L=Lysate load; FT=Flow-through; E=Elution.

Pierce Glutathione Agarose facilitates high yield and high purity GST-fusion protein purification.

How to Use video for Pierce Chromogenic Endotoxin Quant Kit

Use this sensitive, broad range endotoxin quantitation kit to measure endotoxin contamination between 0.01 - 1 EU/mL.

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