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Molecular profiling of cancers is becoming more and more important in the research area of personalized cancer therapy, or precision oncology. Next-generation sequencing (NGS) provides a valuable method for high-throughput applications when many targets and samples need to be multiplexed and screened. However, for very focused applications such as single-analyte or single-gene targets, there is a clear need for fast, simple, and affordable methods for detecting low-level somatic variants. The improved sensitivity achieved using Applied Biosystems Minor Variant Finder Software makes Sanger sequencing an ideal technology for filling this gap.
Details of this application of Sanger sequencing can be found in the application note featured below, which demonstrates:
This study demonstrates how the improved sensitivity achieved through Minor Variant Finder Software makes Sanger sequencing an ideal choice for oncology research applications.
Our simple and fast Sanger sequencing workflow can be completed in less than one workday, from sample to answer. We offer Applied Biosystems products to support many steps of our recommended workflow, from PCR amplification to data analysis. Learn more about each step of our recommended Sanger sequencing workflow.
Get bite-sized answers to your everyday Sanger sequencing and fragment analysis questions
Learn about the history of sequencing and how to pick the right platform for your research needs
For Research Use Only. Not for use in diagnostic procedures.