Capillary electrophoresis is the technology used to conduct Sanger sequencing and fragment analysis with single-base resolution; it can discriminate between single-base changes In DNA length. Still considered the gold standard for DNA sequencing, this simple and fast technology uses fluorescently labeled oligonucleotide primers to enable targeted sequencing and fragment analysis.

sanger sequncing basics

Sanger sequencing basics

Sanger sequencing is a simple and fast sequencing workflow used to study a small subset of genes linked to a defined phenotype, confirm next-generation sequencing (NGS) variants, detect minor allele fractions down to 5%, or read contiguous sequences up to 1,000 bases.

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fragment analysis

Fragment analysis basics

Fragment analysis is a powerful technique with simple, straightforward workflows in which fragments of DNA are tagged using fluorescently labeled primers designed for specific regions of interest and are separated using capillary electrophoresis and sized by comparison to a size standard. This technology can be used for a for a wide variety of DNA sizing applications and is a flexible and effective technique that allows you to multiplex and analyze multiple samples in parallel.

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Additional resources

For Research Use Only. Not for use in diagnostic procedures.