Flow Cytometry Essentials

Viability dyes

Dead cells often give false positive results, as they tend to bind non-specifically to many reagents. Therefore, removing dead cells from your flow cytometry data is a critical step to ensure accurate results and analysis. Invitrogen cell viability assays for flow cytometry help researchers distinguish live and dead cell populations more accurately than only using forward and side scatter characteristics.

Cell proliferation assays

Cell proliferation analysis is important for drug development and biological processes. Proliferation measurements are typically made based on average DNA content or on cellular metabolism parameters. Assays can report either total or live cell numbers, or measure DNA synthesis in single cells. We offer dyes, kits, and antibodies to track proliferation.

Compensation beads

Compensation beads capture species-specific antibodies conjugated to fluorophores and other types of reagents. The purpose of these beads is to set voltages and gating parameters for obtaining accurate fluorescence signal.

Conjugated antibodies

Build your flow cytometry panels with our antibodies to identify cells and detect proliferation, homing profiles, activation states, and cytokine release. We offer antibodies conjugated to many types of fluorophores to accommodate your flow cytometry needs.

Nunc UpCell Surface Plastics

UpCell surface is a temperature-responsive cell culture surface that enables non-enzymatic harvesting of adherent cells for preservation of cell viability and surface proteins.

Simply transfer the culture vessel to room temperature to collect cells in suspension or in the form of a "cell sheet" with the help of a supportive membrane.

Nunc Serological Pipettes

Stringent quality specifications and convenient features make Thermo Scientific Nunc Serological Pipettes a great choice for daily lab work. Bold, clear, bi-directional graduations make it easy-to-read fluid volumes and dispensing amounts. Choose from a broad selection of sizes and packaging options.

Nunc Conical tubes, 15 and 50 ml

Nunc 15 mL and 50 mL Conical Sterile Polypropylene Centrifuge Tubes are premium, high-quality conical tubes that are environmentally friendly, offer the highest cleanliness with a recyclable plastic rack, and allow for increased traceability with the largest writing area on the market.

Nunc Cell Culture Dishes

Cell culture dishes come in wide range of sizes and styles as well as format, material, and surface modification. Each is designed and produced with highest quality standards to promote healthy cells and reproducible results.

Invitrogen Accutase Enzyme Cell Detachment Medium

Accutase performs exceptionally well in detaching cells for analysis of cell surface markers, virus growth assay, quiescence assays by serum starvation, transformation assays by oncogene transfection, neural crest cell migration assays, cell proliferation, cell haptotaxsis, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry.   

Gibco Versene Solution

Versene solution is an EDTA solution for use as a gentle non-enzymatic cell dissociation reagent. Gibco Versene Solution (0.48 mM) is formulated as 0.2 g EDTA(Na₄) per liter of Phosphate Buffered Saline (PBS).

Gibco Cell Dissociation Buffers

Gibco Cell Dissociation Buffers are membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free phosphate-buffered saline (PBS). Gibco Cell Dissociation Buffer is suitable for the gentle dissociation of mammalian cells for studies such as ligand binding and flow cytometry.

Nunc Cell Scraper

Featuring an adjustable blade, the sterile, nonpyrogenic Thermo Scientific Nunc Cell Scraper allows for convenient mechanical harvesting of the adherent cells from culture vessels. It comes in two lengths to accommodate a wide range of culture wares.

Dynabeads FlowComp beads

Eliminate the risk of contaminating and incubating your cells with iron, dextran, or other potentially harmful structures. FlowComp beads allow for positive cell isolation directly followed by cell release and removal of the magnetic beads.

Sample preparation buffers

The sample buffer fixation and permeabilization buffer sets are designed for use in intracellular staining and flow cytometric analysis and has been specially formulated to reduce non-specific staining of fluorochrome-labelled antibodies and increase fluorescence signal to noise ratios.  Achieve the best possible results with buffer sets, cell lysis and preservation assays, blocking reagents, and magnetic cell isolation beads.

Gibco Balanced Salt Solutions

All Gibco balanced salt solutions are manufactured in state-of-the-art cGMP, ISO certified facilities to ensure the highest quality and consistency for reproducible results every day. Tests include osmolality, pH, stability, the absence of bacterial and fungal contamination, and endotoxin tests. All powdered balanced salt solutions are produced without sodium bicarbonate to increase stability.