Probe Labeling Chart | Thermo Fisher Scientific

Probe Type	Application	Starting Material for Probe Synthesis	Probe Synthesis Reaction	Ambion Kit	Comments
dsDNA (I,N)	Northern, Southern, dot blots	cDNA inserts, PCR product, plasmid with insert	Random priming reaction	DECAprime™ II	Fastest reaction Least sensitive
ssRNA (I,N)	NPA, in situ hybridization	Plasmid with insert and polymerase promoter	In vitro transcription	MAXIscript™	For use in ribonuclease protection assay
Oligonucleotide (I)	Northern, Southern, dot blots	Oligonucleotide	End labeling	KinaseMax™	Fastest reaction Least sensitive
Oligonucleotide (I)	NPA	Oligonucleotide	End labeling	KinaseMax™	Fastest reaction Least sensitive
ssRNA ssDNA dsDNA Oligonucleotide (N)	Northern, Southern, Dot blots, in situ hybridization	DNA, RNA or oligonucleotide	Chemical labeling, post-nucleic acid synthesis	BrightStar™ Psoralen-Biotin	Sensitivity depends on nucleic acid labeled (e.g., ssRNA vs. dsDNA)

(I) Isotopic
(N) Nonisotopic