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High quality viral RNA for veterinary assays is essential for rapid viral identification. The ability to perform high throughput viral RNA isolation from diverse samples also increases laboratory efficiency, reduces labor cost, and improves viral detection. This article describes how the Ambion® MagMAX™ magnetic bead-based technology has been successfully adapted for automated sample processing on the Caliper Life Sciences Sciclone® ALH 3000 Workstation for nucleic acid isolation from diverse samples.
High throughput viral RNA isolation from diverse samples enables rapid viral detection in animal samples. The Ambion MagMAX-96 Viral RNA Isolation Kit is designed for viral RNA/DNA purification from a variety of sample types, such as animal tissues, blood, serum, plasma, milk, swabs (e.g., oral, cloacal), and feces (see sidebar, Challenges of RNA Isolation From Cell-free Systems). Small elution volumes (20–50 µL) provide concentrated, purified nucleic acids for convenient streamlining of downstream applications.
Applied Biosystems scientists with the Custom Services group (see article, Accelerate Your Research with Custom Solutions) have validated the MagMAX-96 Viral RNA Isolation Kit for use on an automated sample handling platform, the Sciclone ALH 3000 Workstation (Caliper Life Sciences). RNA was isolated from Armored RNA® particles (Asuragen, Inc.)—synthetic viral particles containing an RNA oligonucleotide—spiked into diverse biological sample matrices, including human plasma, bovine serum, raw bovine milk, and brain heart infusion (BHI) medium, which is commonly used for swab sample storage.
Recovery of RNA from Armored RNA® particles was highly consistent across the various matrix types, and no cross-contamination was observed (Figures 1–2). The data clearly indicate that the combined use of the MagMAX-96 Viral RNA Isolation Kit on the Caliper Life Sciences Sciclone ALH 3000 Workstation provides a quick and robust workflow for efficient, high throughput viral RNA/DNA isolation.