Click Chemistry and Other Functional Group Modifications—Chapter 3

Click Chemistry—Section 3.1

• Principles of Bioorthogonal Labeling and Click Chemistry
• Copper-Free Click Chemistry
• Click-iT Azide and Alkyne Labeling Reagents
• Click-iT Tools for Cell Proliferation Analysis
• Click-iT TUNEL Assay
• Click-iT Tools for Detecting RNA and Protein Synthesis
• Click-iT Tools for Detecting Lipid Peroxidation

Reagents for Modifying Alcohols—Section 3.2

• Alcohols in Proteins: Serine, Threonine and Tyrosine Residues
• Alcohols in Carbohydrates
• Alcohol Modification in Organic Solvents

Reagents for Modifying Aldehydes and Ketones—Section 3.3

• Introducing Aldehydes and Ketones into Biomolecules
• Introducing a Hydroxylamine into a Biomolecule
• Hydrazines and Hydroxylamines
• Aliphatic and Aromatic Amines

Derivatization Reagents for Carboxylic Acids and Carboxamides—Section 3.4

• Coupling Hydrazines, Hydroxylamines and Amines to Carboxylic Acids
• Esterification of Carboxylic Acids with Fluorescent Diazoalkanes
• Fluorescent Alkyl Halides
• Fluorescent Trifluoromethanesulfonate
• 4-Sulfo-2,3,5,6-Tetrafluorophenol (STP) and N-Hydroxysulfosuccinimide (NHSS)

Related Tables

• Molecular Probes azide and alkyne derivatives—Table 3.1
• Molecular Probes hydrazine, hydroxylamine and amine derivatives—Table 3.2