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Spheroid generation on AlgiMatrix plates
Plate the cells at a density of 20,000 - 80,000 cells/well in triplicates in 100 ml complete media on AlgiMatrix plate. Spin the plate at 100 x g for 4 minutes. Incubate the plate at 37°C in a 5% CO2 incubator for 10 minutes. Add 130 ml of complete media subsequently into each well.
In Situ harvesting of spheroids by tri-sodium citrate method
Preparation of iso-osmolar tri-sodium citrate/glucose solution
Note: Adding 1g/L NaCl to the solution will raise the osmolarity by 30 mOsm
For Research Use Only. Not for use in diagnostic procedures.