Mass Spectrometry Standards and Calibrants Support—Troubleshooting

We recommend checking your mass spectrometry system performance using the Pierce HeLa Protein Digest Standard (Cat. No. 88328) to help determine if the problem is from the sample preparation or the LC-MS system.

We recommend using thePierce Peptide Retention Time Calibration Mixture (Cat. No. 88321). It provides synthetic heavy peptides to diagnose and troubleshoot your LC (liquid chromatography) system and gradient.

Your mass spectrometry instrument may require calibration. We recommend recalibrating using one of our Pierce Calibration Solutions. Additionally, verify that correct search parameters were used for database searching (e.g., species, enzyme, fragment ions, mass tolerance, etc.)

Here are some recommendations:

• Clean and recalibrate the mass spectrometry instrument using one of our Pierce Calibration Solutions.
• Verify settings for liquid chromatography (LC) acquisition methods.
• Fractionate TMT-labeled samples using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Cat. No. 84868) to reduce sample complexity.

Yes, we offer the Pierce Reserpine Standard for LC-MS (Cat. No. 88326) to guide in troubleshooting spray stability.

We recommend using the Pierce HeLa Protein Digest Standard (Cat. No. 88328) to test your sample clean-up method. Use it directly and also as a control co-treated with the sample to check for any loss of peptides.

Yes, Pierce Reserpine Standard for LC-MS (Cat. No. 88326) can be used to assess instrument sensitivity.