Having difficulties with your experiment?

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General

What does “Error - Calibration file already exists” mean?

The calibration files are stored on the QuantStudio™ instrument itself; therefore, when you need to perform a new calibration, you will have to delete the old calibration files. From the instrument console, go to the QuantStudio™ instrument touchscreen and select ‘Collect Results’. You should then see a list of files stored on the instrument. Manually select a calibration file such as “Fast-96-cal”, and press the ‘Delete’ button to remove the old calibration file. Occasionally, users cannot delete the file off the instrument and may encounter this error: “Some experiments did not collect results therefore cannot be deleted.”. In these situations, launch the software, go to ‘maintenance’, manage files, and download the calibration file. The status of the experiment should be displayed as ‘complete’. Next, delete the old calibration file and rerun the calibration.

What can I do when I get an error message related to “…JVM…”?

This error can occur due to allocation of the computer’s virtual memory. If you see this message, try the following:

  1. Click ‘OK’ to the error, close any unnecessary programs, and re-launch the software (you may have to do this several times).
  2. Using the Task Manager (Control+Alt+Delete), look for a process called ‘javaw.exe’. Select this line and end the process. Then re-launch the software.
What should I do if the software will not connect to the QuantStudio™ 6 or QuantStudio™ 7 Real-Time PCR System?

After launching the QuantStudio™ software, go to the Maintenance Manager. Check if the QuantStudio™ system icon is present in the “On the Network” window. If not, click on the ‘Refresh’ button to the left of ‘Remove from My Instruments’. If you still do not see the QuantStudio™ system icon for the following reasons:

  • There is an icon but you cannot add it into the "My Instruments" area
  • There is a red “X” on the instrument
  • A message says "Disconnected" at the bottom of the window with a red “X” in it

then you will need to add the Instrument into the ‘My Instruments’ area. Click on the wrench icon in the upper right-hand corner of the icon as pictured below. You may get a message that says the firmware is older than the one you're trying to run. If appropriate, choose to download the new firmware and let it progress to finish. Do not turn off the instrument during this update. After the firmware update is completed, restart the instrument and check for the QuantStudio™system icon in ‘On the Network’ area.

With the QuantStudio™ 6 and 7 Flex Real-Time PCR System Software, v1.0, I get the error message “Unable to start the run” when starting a new run. What can I do?

We recommend that you send the run file to the QuantStudio 6 Flex or QuantStudio 7 Flex Real-Time PCR instrument and start the run from the instrument touchscreen. Additionally, you can upgrade to v1.3 or above of the QuantStudio Real-Time PCR software and you will not have this error message show up.

How do I activate or renew my software license in the QuantStudio™ 6 or QuantStudio™ 7 Real-Time PCR System?

Please follow the instructions found in this User Bulletin: How to Activate or Renew a License. (Note: This bulletin also applies to the QuantStudio™ instruments even though the example provided refers to the ViiA™ 7 instrument). If you have a license key associated with the computer that is no longer in use, please contact our Instrument Services group at instrumentservices@lifetech.com with the serial number and license key so that they can reassign it for you.

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For Research Use Only. Not for use in diagnostic procedures.