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The CTS Rotea System is well positioned to provide high purity and cell viability for cell processing of many different cell types.
Cell samples washed and concentrated using the Rotea instrument exhibit excellent viability and recovery in small volumes. T cell quality and composition are maintained after Rotea instrument processing, and PBMC isolation can be completed in much less time than is possible with high density media protocols.
The low volumes of efficiently washed and concentrated cells from the CTS Rotea system can be transferred directly to the CTS Xenon Electroporation System for transfection steps. Using these two highly compatible Cell Therapy systems saves times and reduces workflow complexity.
To learn more about automated PBMC isolation, T cell wash and concentration, and CAR T cell manufacturing using the CTS Rotea system and how the Rotea and Xenon systems can work together to support highly efficient and effective cell therapy workflows, use the link below to see all CTS Rotea system application notes.
Even at high input volumes, the Rotea system allows for rapid cell processing with high cell recovery. In less than four hours, users can effectively wash and concentrate large volumes of T cells or separate PBMCs from leukopak bags.
Input volume (L) |
Processing time (min) |
Cell recovery |
---|---|---|
T cell wash and concentrate (1 x 106 cells/mL) | ||
0.5 | 11 | >90% |
1.0 | 18 | |
3.0 | 38 | |
5.0 | 58 | |
10.0 | 114 | |
20.0 | 226 |
Input volume (L) |
Processing time (min) |
Cell recovery |
---|---|---|
PBMC isolation | ||
~0.25 (¼ leukopak bag) | 25 |
>90% |
~0.5 (½ leukopak bag) | 45 |
|
~1.0 (full leukopak bag) | 85 |
|
5 | 116 |
|
10 | 230 |
Flow cytometry data shows that PBMC cell separation in less than 30 minutes with the CTS Rotea instrument produces similar results to a two-hour Ficoll media separation.
The CTS Rotea system can also perform successful processing of NK cells at flow rates up to 80 mL/min, including automated washing and concentration of human NK cells expanded in Gibco CTS NK-Xpander Medium. In addition to high viability and recovery of NK cells, these cells also maintained their cytotoxicity following processing.
MSCs have emerged as a promising cell type for cell therapy aimed at treating a variety of diseases. Closed and automated cell processing systems such as the Rotea system can support the manufacture of MSC-based cell therapies by promoting scalability, regulatory compliance, and quality control.
This includes the use of a custom StemPro SFM XF media for expansion of mesenchymal stem cells (MSCs) in monolayer and microcarrier suspension-based cultures and use of closed system passaging and harvesting of MSCs from multi-layered flasks using the Rotea system.
MSCs harvested from Cell Factory Systems using the Rotea system have been shown to maintain high cell viability and expression of known MSC markers.
In addition to the data presented here, researchers have designed a semi-automated process using counterflow centrifugation to passage and harvest MSCs from multi-layered flasks.
Gentle counterflow centrifugation using the Rotea system allows for reduction in turbidity levels during lentiviral clarification to levels comparable to manual centrifugation but in a closed, low shear environment. The Rotea system enables faster processing times between 25-75 mL/min for larger scale applications and furthermore, processing using the Rotea system results in minimal loss of infectious titer.
The CTS Rotea system provides an efficient and effective closed system for the dissociation of pluripotent stem cell (PSC) spheroids grown in suspension cultures. Utilizing this closed-system environment significantly decreases the risk of contamination compared to water bath methods.
Prior to use for cell therapy, cells may be thawed, selected, expanded, genetically altered, differentiated, and/or cryopreserved. Each of these steps may require a different medium or buffer that can contain substances harmful to cells. The CTS Rotea system can effectively remove residual substances with minimal cell loss.
For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products.