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Instrument specifications
Easily fitting on a benchtop, the compact CTS Rotea system’s counterflow centrifugation technology was designed to gently and efficiently separate cells or aggregates based on size, and to concentrate particles with outer diameter (OD) >5 μm.
The proprietary Gibco DirectDraw extraction technology allows users to gently and precisely recover washed and concentrated cells from the processing chamber at the extremely low-volume output of 5 mL and is ideal when electroporation is the next step. In addition, the CTS Rotea system incorporates multiple sensors to monitor and protect the integrity of the closed system.
| Attribute | Description |
| Centrifugal force | 3000 x g |
| Peristaltic pump flow rate | 5–160 mL/min* |
| Centrifuge chamber volume | 10 mL |
| Cell count per chamber load | 5 x 10⁷–5 x 10⁹ cells** |
| Output cell concentration | 5 x 10⁶–500 x 10⁶ cells/mL |
| Recommended input volume range | 50 mL–20 L |
| Minimum output volume range | 5 mL |
| Maximum fluid density | 1.10 g/mL |
| Operating noise level | <70 dBA |
| Dimensions (H x W x D) | 16 x 11.4 x 20 in (body only), 30 x 11.4 x 20 in (with bag poles) (40 x 29 x 50.8 cm (body only), 76.2 x 29 x 50.8 cm (with bag poles)) |
| Weight | 44 lb (20 kg) |
| Tablet accessory model | Dell™ system, validated by Thermo Fisher Scientific for use with the CTS Rotea system |
*Dependent on single-use kit type.
**Dependent on cell type. Centrifuge can be filled multiple times to process larger batches.
Note: Specifications and parameters above are cell type and protocol dependent. Instrument and Consumable Regulatory Support File available upon request at thermofisher.com/regulatory.
Single-use kit specifications
The CTS Rotea single-use kit provides flexibility for process development and seamless transfer to clinical-scale manufacturing.
| Parameter | Specification |
| Kit sterility | Compatible with Luer connectors and sterile welding |
| Sterilization method | Gamma sterilized |
| Tubing material | DEHP-free PVC |
| Flow rates | Standard kit: 5–110 mL/min Hi-flow kit: 30–160 mL/min |
| Output cell concentration | Up to 500 x 106 cells/mL |
For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products.