Pharmacogenomic Copy Number Variant (CNV) Analysis

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Analyze CYP2D6 copy number variants with the QuantStudio™ 12K Flex Real-Time PCR System. TaqMan® Copy Number Assays use TaqMan® MGB probe chemistry to obtain specific, reproducible, and easy-to-interpret copy number results for important pharmacogenomic genes such as CYP2D6.

Life Technologies provides a broad product family to extend your research to other important pharmacogenomic variants:

Predesigned TaqMan® Copy Number Assays
- Human: over 1.6 million predesigned assays available for genome-wide coverage
- Mouse: over 180,000 predesigned assays available for gene exons
Custom Plus TaqMan® Copy Number Assays
- Submit user-defined human or mouse genomic targets for custom assay design, including upfront bioinformatic sequence analysis and in silico QC
Custom TaqMan® Copy Number Assays for other targets of interest
- Submit any masked target sequences for assay design
- Submit primer/probe pair sequences for assay synthesis

Experimental Workflow: Find the Products You Need for Every Step

Step 1Prepare the DNA
Step 2Select the assay
Step 3Prepare the reaction
Step 4Amplify and read
Step 5Analyze the data

Purify the DNA using MagMAX™ technology

Recommended products:

MagMAX™ DNA Multi-Sample Kit
MagMAX™ DNA Multi-Sample Kits are designed for rapid purification of high-quality genomic DNA from a variety of sample types including blood, blood cards, and buccal swabs. These purification kits use MagMAX™ magnetic bead–based nucleic acid isolation technology to produce high yields of purified DNA, free from inhibitors that may affect downstream reactions such as genotyping assays.
MagMAX™ Express Magnetic Particle Processors
MagMAX™ accessories

Select your TaqMan® Copy Number Assays using our assay search tool.

Recommended products:

Perform real-time plate amplification and read using the QuantStudio™ 12K Flex system with the 384-well, Fast 96-well, or 96-well block.

Whether you need installation qualification and operation qualification (IQ/OQ) or instrument performance verification (IPV), our qualification services help you verify that your instrument is working according to manufacturer’s specifications, thereby allowing you to produce audit-quality data to share with regulatory agencies.

Recommended products:

CopyCaller® Software analyzes data generated on the QuantStudio™ 12K Flex platform to determine the number of copy variants for each target analyzed. AlleleTyper™ Software converts copy number results into star-allele results.

CopyCaller® Software can:

Open copy number experiment absolute quantitation data collected by most real-time instruments from Life Technologies
Perform copy number analyses with or without a known calibrator sample
Report confidence in individual or grouped copy number calls
Display the calculated sample copy number data in both table and graphic formats
View detailed data analysis information
Change analysis parameters and reanalyze the data
Simultaneously analyze and view data from multiple experiments
Save or print analyzed data for further analysis or for sharing with other laboratories that use CopyCaller® Software

Recommended products:

MagMAX™ DNA Multi-Sample Kit
MagMAX™ DNA Multi-Sample Kits are designed for rapid purification of high-quality genomic DNA from a variety of sample types including blood, blood cards, and buccal swabs. These purification kits use MagMAX™ magnetic bead–based nucleic acid isolation technology to produce high yields of purified DNA, free from inhibitors that may affect downstream reactions such as genotyping assays.
MagMAX™ Express Magnetic Particle Processors
MagMAX™ accessories

Step 2 : Select the assay

Select your TaqMan® Copy Number Assays using our assay search tool.

Recommended products:

Step 4 : Amplify and read

Perform real-time plate amplification and read using the QuantStudio™ 12K Flex system with the 384-well, Fast 96-well, or 96-well block.