Cyclophilin D (CyPD) is a member of the cyclophilin family of peptidylprolyl isomerases. Cyclophilin D is located in the matrix of the mitochondria where it acts as an integral member of the permeability transition pore complex, which also includes the voltage dependent anion channel (VDAC) and the adenine nucleotide translocator (ANT). Activation of this pore is involved in the induction of apoptotic and necrotic cell death. Cyclophilin D is conserved, ubiquitous, and abundantly expressed in most cells, making it a good loading control. We offer a number of quality Invitrogen cyclophilin D antibodies for a variety of research needs.

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Applications
Cyclophilin D Antibody PA3-022 immunofluorescence data

Immunofluorescence analysis of cyclophilin D in A431 cells. Cells were probed with a Cyclophilin 40 Polyclonal Antibody (Cat. No. PA3-022) at a dilution of 1:200, or treated equivalently without primary antibody (negative control) and incubated with a Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 488 (Cat. No. 35503). The resultant staining of cyclophilin D (green), F-actin with phalloidin (red), and nuclei with DAPI (blue) is shown.

Immunohistochemical analysis was performed on deparaffinized human hepatocarcinoma biopsy tissues. Tissues were probed with a Cyclophilin 40 Polyclonal Antibody (Cat. No. PA3-022) or treated equivalently without primary antibody (negative control). Tissues were counterstained with hematoxylin and prepared for mounting.

Cyclophilin D Antibody PA3-022 immunohistochemistry data

Resources

For Research Use Only. Not for use in diagnostic procedures.

For Research Use Only. Not for use in diagnostic procedures.