Human CD4+ T Cell Isolation | Thermo Fisher Scientific

Which Human CD4+ T Cell Isolation Product is Right for You?

	Highest purity for molecular assays	Affordable & pure, flow compatible cells	Extreme purity & physiological quality	Untouched - most physiological relevant cells
	Dynabeads® CD4	Dynabeads® FlowComp™ Human CD4	Dynabeads® CD4 Positive Isolation Kit	Dynabeads® Untouched™ Human CD4 Cells
Isolation Method	Depletion or positive isolation	Positive isolation	Positive isolation	Negative isolation
Starting samples	Any sample (whole blood, MNC, bone marrow)	Any sample (whole blood, MNC, bone marrow)	Any sample (whole blood, MNC, bone marrow)	MNC*
# MNC processed (mL whole blood processed)	1-2 x 10⁹ MNC 200 mL whole blood	2 x 10⁹ MNC 80 mL whole blood	2 x 10⁹ MNC 400 mL whole blood	1 x 10⁹ cells
Purity	Highest 98-100%	Higher 95-99%	Highest 98-100%	High 90-95%
Yield	Highest 95-100%	High 70-85%	Standard 50-70%	Standard 50-70%
Viability	>95%	>95%	>95%	>95%
Isolation Time	~30 min	~60 min	~90 min	~50 min
Isolation Temp.	Cold	Cold	Release at room temp.	Cold
Applications	Depletion of unwanted CD4+ T or positive isolation for molecular applications, e.g. isolation of proteins & nucleic acids	Cellular assays (cell culture, cell activation/proliferation, cytokine studies) Molecular isolation of proteins & nucleic acids	Cellular assays (cell culture, cell activation/proliferation, cytokine studies) Translational research Molecular isolation of proteins & nucleic acids	Cellular applications for physiological studies (functional studies, flow analysis, study of resting/activated cells)
Bead-free Target Cells	No	Yes (but antibody present)	Yes (and antibody-free)	Yes
Compatible with Flow Cytometry /Sorting	No	Yes	Yes	Yes
Shelf Life from Production Date	3 years	2 years	3 years	2 years
Required Materials	Magnets	Magnets	Magnets	Magnets
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*MNC - Mononuclear cells

THIS IS FOR HUMAN RESEARCH - NOT FOR MOUSE