Amine-reactive, orange- and red-fluorescent fluorophores—Table 1.9 | Thermo Fisher Scientific - BR

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Table 1.9 Amine-reactive, orange- and red-fluorescent fluorophores.

Fluorophore (Abs/Em) *	Succinimidyl Ester	Other	Protein Labeling Kits	Notes
Lissamine rhodamine B (570/590)		L20 (SC)^M L1908 (SC)^M		Optimal for 568 nm excitation Photostable
Naphthofluorescein (602/672)	C653 ^M	C652 (COOH)^M		Very long-wavelength excitation and emission pH-sensitive fluorescence, with a high pK_a (~7.6)
Rhodamine 6G (525/555)	C6127 ⁵ C6128 ⁶ C6157 ^M			Excited by the 514 nm spectral line of the argon-ion laser Spectra intermediate between those of fluorescein and tetramethylrhodamine
Rhodamine Red-X (580/590)	R6160 (X)⁵		F6161 (F)	Conjugates of Rhodamine Red-X are generally more fluorescent than those of Lissamine rhodamine B, and the succinimidyl ester is more stable in H₂O
Tetramethylrhodamine (555/580)	C2211 ⁵ C6123 ⁶ C1171 ^M T6105 (X)^M	C6121 (COOH)⁵ C6122 (COOH)⁶ C300 (COOH)^M T1480 (ITC)⁵ T1481 (ITC)⁶ T490 (ITC)^M		pH-insensitive fluorescence Good photostability Conjugates are prone to aggregation Succinimidyl ester derivative (6-TAMRA, SE; C6123) is widely used for oligonucleotide labeling
Texas Red dye (595/615)	T6134 (X)^M T20175 (X)^S	T353 (SC)^M T1905 (SC)^MT30200 (DTA)^M	F6162 (F) T10244 (P) Z25045 (Z)	Good spectral separation from green fluorophores Texas Red-X succinimidyl ester typically yields greater fluorescence per attached dye than Texas Red sulfonyl chloride and is more stable in H₂O
X-rhodamine (580/605)	C6125 ⁵ C6126 ⁶ C1309 ^M	C6124 (COOH)⁵ C6156 (COOH)⁶ X491 (ITC)^M		Succinimidyl ester derivative (6-ROX, SE; C6126) is widely used for oligonucleotide labeling
* The numbers in parentheses reflect the absorption (Abs) and fluorescence emission (Em) maxima, in nm, of the goat anti–mouse IgG antibody or dextran conjugates in aqueous buffer. Superscripts 5, 6 and M correspond to 5-isomer, 6-isomer and a mixture of 5- and 6-isomers, respectively. (COOH) = Carboxylic acid. (F) = FluoReporter Protein Labeling Kit (Kits for Labeling Proteins and Nucleic Acids—Section 1.2). (ITC) = Isothiocyanate. (P) = Easy-to-Use Protein Labeling Kit (Kits for Labeling Proteins and Nucleic Acids—Section 1.2). (S) = Single isomer. (SC) = Sulfonyl chloride. (X) = Aminohexanoyl spacer separating the dye and the SE. (Z) = Zenon Antibody Labeling Kit (Zenon Technology: Versatile Reagents for Immunolabeling—Section 7.3).

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