Lipofectamine® 3000 Transfection Reagent:
Lipofectamine® 3000 reagent leverages our most advanced lipid nanoparticle technology to enable superior transfection performance and reproducible results. It delivers exceptional transfection efficiency into the widest range of difficult-to-transfect and common cell types with improved cell viability.

Lipofectamine® 2000 Transfection Reagent:
A proprietary cationic lipid formulation that offers the highest transfection efficiencies and protein expression levels on the widest variety of adherent and suspension cell lines

Cotransfecting RNAi and DNA with Lipofectamine 2000:
This reference provides general guidelines and a procedure to co-transfect plasmid DNA and an RNAi molecule (i.e. Stealth™ RNAi, siRNA, shRNA plasmid or miRNA plasmid) into mammalian cells using Lipofectamine™ 2000 Reagent

Lipofectamine 2000 Reverse Transfection:
Reverse transfection combines RNA, transfection reagent, and cells in an altered sequence compared to traditional Lipofectamine™ 2000 transfection protocols; in short, a different RNAi molecule is put in each well prior to transfection and combined with diluted Lipofectamine™ 2000 to form complexes in each well; cells are added directly to the Lipofectamine™ 2000-RNA complexes and transfection occurs while cells are attaching to the well

Lipofectamine® 2000 CD Transfection Reagent:
Designed for applications where concerns about animal-derived components exist; synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in experiments or cell lines

Lipofectamine® LTX Reagent:
Offers a new solution for gene expression; no other plasmid DNA-specific transfection reagent can provide the same level of reproducible and efficient gene expression in a wide range of cell lines, including primary and disease-related cells

Lipofectamine® LTX Reagent Cell-type Specific Protocols:
These protocols provide a recommended procedure to transfect plasmid DNA into different types of cells

PLUS™ Reagent:
Used in conjunction with transfection reagents, such as Lipofectamine™ LTX, to enhance transfection in adherent and suspension cell lines

Transfecting Plasmid DNA into Primary Mouse Neural Progenitor Cells Using Lipofectamine® LTX Reagent:
This reference provides a customer-generated protocol for transfecting plasmid DNA into primary mouse neural progenitor cells. Please note that this protocol has not been verified by scientists at Invitrogen. It is provided as references of transfection in challenging cell type to share with all customers. Further optimization of this protocol might be necessary in individual laboratories

Transfecting Plasmid DNA into RAW264.7 Macrophage Cells Using Lipofectamine® LTX Reagent:
This reference provides a customer-generated protocol for transfecting plasmid DNA into Raw 264.7 Macrophage cells. Please note that this protocol has not been verified by scientists at Invitrogen. It is provided as references of transfection in challenging cell type to share with all customers. Further optimization of this protocol might be necessary in individual laboratories

Lipofectamine® RNAiMAX Transfection Reagent:
A proprietary RNAi-specific cationic lipid formulation that offers the highest transfection efficiencies on the widest variety of cell types for siRNA gene knockdown experiments

Lipofectamine® RNAiMAX Transfection Reagent Cell-type Specific Protocols:
These protocols provide a recommended procedure to transfect siRNA into different types of cells

FreeStyle™ MAX Reagent:
A breakthrough technology for rapid and high-yield mammalian protein production in suspension - adapted CHO and 293 cells.

293fectin™ Transfection Reagent:
A specially designed cationic-lipid formulation which offers extremely high transfection efficiencies of FreeStyle™ 293-F suspension cells in serum-free GIBCO® FreeStyle™ 293 Expression Medium

Oligofectamine™ Transfection Reagent:
Forms stable complexes with oligos, permitting efficient transfection into eukaryotic cells in a highly-specific, non-toxic fashion

Lipofectamine® Transfection Reagent:
One of our first generation cationic-lipid transfection reagents formulated for the transfection of DNA into eukaryotic cells

Lipofectin® Transfection Reagent:
The reagent of choice for transfectin of endothelial cells; some of our customer also use it for transfection of DNA, RNA or oligonucleotides into their cell line of choice; it has also been shown to work well in combination with PLUS® reagent for the transfection of HeLa cells

DMRIE-C Transfection Reagent:
A liposomal formulation which complexes with nucleic acids for efficient transfection into mammalian cells

Optifect™ Transfection Reagent:
Pre-optimized formulation designed to ensure highly efficient gene delivery to a wide variety of cell lines transfected at low confluence or density (10%-70%)

Calcium Phosphate Transfection Kit:
Provides high-quality reagents to enable the introduction of DNA into eukaryotic cells via calcium phosphate co-precipitation

Cellfectin® Transfection Reagent:
A cationic-lipid formulation designed for optimal transfection of insect cells