GeneArt® TALEN Products and Services | Thermo Fisher Scientific

GeneArt® TALEN Transcription activator–like effector nuclease (TAL, TALE, or TALEN) proteins are naturally occurring transcriptional activators secreted by Xanthomonas spp. into their plant hosts. The deciphering of the TAL effector “code” led to the engineering of designer TAL effector proteins, a new approach to genome editing. Whether through targeted nucleases for genome engineering, or by precisely directed moderators of gene expression, GeneArt® Precision TALs are already helping advance a broad range of life science applications, including cell, molecular, and synthetic biology; drug discovery; and biofuels research.

Learn more about TALEN-based editing

Design with precision

GeneArt® Precision TALs provide a custom DNA-binding proteins for accurate DNA targeting and precise genome editing. Unlike other technologies that limit the choice of targets, or provide ambiguous results, GeneArt® Precision TAL enables the targeting of any locus in the genome. GeneArt® Precision TALs offers site-specific delivery of nucleases, activators, repressors, chromatin modifiers, genomic labels, and cross-linking molecules. This genome editing technology is known to function in a variety of host systems, including bacteria, yeast, plants, insects, zebrafish, and mammals.

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Precise genome editing for every gene locus is now within your reach

Free TALEN Design Consultation

TALEN Design Support Request

Custom TALEN Cell Line Service

Request information about GeneArt® Precision TALs

Ordering GeneArt® Precision TALs

The fastest and easiest way to design, edit, optimize, and order GeneArt® Precision TALs is through the GeneArt® portal.

Order online

The ordering process is simple; just follow the three steps below:

Select the functionality (effector domain) of your TAL from Table 1. For details, refer to the ‘TALEN functionality overview’
Select the product name from Table 1.
Then place your order through our online design tool within the GeneArt® portal, or you can downloadand complete the TALEN order form and email it to geneartsupport@lifetech.com.

If you have a question or need free design consultation, contact us here and we’ll be happy to assist.

We will ship you a clone with a verified, optimized sequence approximately 2 weeks after confirming your order.

Table 1: Available effector domains

Product	DNA-binding domain	Effector domain	Functionalities	Applications
Native TALEN™ Fok1	Native	Fok1 endonuclease	Gene targeting	Silencing Incorporation of exogenous DNA
Truncated TALEN™ Fok1	Truncated	Fok1 endonuclease	Gene targeting	Silencing Incorporation of exogenous DNA
Native TAL VP16 activator	Native	VP16 activator	Activation of transcription	Increasing the expression level of endogenous gene isoforms
Native TAL VP64 activator	Native	VP64 activator	Activation of transcription	Increasing the expression level of endogenous gene isoforms
TAL repressor	Modified	KRAB repressor	Epigenetic repression of transcription	Heritable knockdown of gene expression
Native TAL MCS	Native	(MCS)	Steric repression & custom design	Transient knockdown of gene expression Target any locus in the genome with the effector domain of your choice
Truncated TAL MCS	Truncated	(MCS)	Steric repression & custom design	Transient knockdown of gene expression Target any locus in the genome with the effector domain of your choice

KRAB: Krüppel associated box; MCS: multiple cloning site; TAL: transcription activator–like (DNA-binding domain).

An essential tool for monitoring the efficiency of your genome editing experiments

When using genome editing tools such as CRISPRs, TALs, or zinc finger nucleases to obtain targeted mutations, it is necessary to determine the efficiency with which these nucleases cleave the target sequence prior to continuing with labor-intensive and expensive experiments. The GeneArt® Genomic Cleavage Detection Kit provides a simple, reliable, and rapid method to determine the nuclease cleavage efficiency at a given locus.