Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Accurate genetic measurements often require comparison to reference samples and assay standards. Standardization of references is especially important in the field of metrology. For many organisms or applications, there is often no suitable reference sample available. Generation of reference standards using conventional real-time PCR requires consideration of how the reference sample will initially be calibrated, its long-term stability, and whether there is sufficient reference material for completion of all future studies. In addition, the lack of broadly adopted standards impacts comparison between laboratories.
Digital PCR does not rely on a reference sample or assay standard; it can be used for absolute quantification, measuring the exact copy number of a nucleic acid target of interest. This capability is especially useful for calibrating reference samples and assay standards when none exist. Through direct copy number determination, digitally measured assay standards can enable laboratories to compare results, with the assurance that measurements are based on the same absolute baseline.
Digital PCR: Absolute Quantification Applications using the QuantStudio™ 3D
Measuring the absolute number of molecules in a biological sample is a relatively common need. But, this can pose some unique challenges when using standard real-time PCR. In contrast, digital PCR is not dependent on cycle thresholds or references, yielding a simple and precise method to obtain absolute quantification.
Product Bulletin: QuantStudio™ 3D Digital PCR System
User Guide: QuantStudio™ 3D Digital PCR System
Digital PCR Experiment Design Guide
QuantStudio™ 3D Digital PCR System
Absolute quantification, using sealed-chip technology, for a reliable method and precise, sensitive data.
For Research Use Only. Not for use in diagnostic procedures.