Solutions in Cancer Research

Study up to 16-data parameters from your precious samples while saving time with the high-performance capabilities of the Invitrogen Attune NxT Flow Cytometer. This dynamic instrument is designed with active mechanical resistance to clogging, novel acoustics-assisted hydrodynamic focusing for speed, and sample acquisition from 96-well and 384-well microtiter plates for reliable automation. Immunophenotyping analysis on minimally processed samples can be performed using a simplified no-wash, no-lyse method, reducing the time-intensive traditional sample preparation protocols from 10 steps to 3 straightforward steps.

Invitrogen CytoVista Tissue Clearing Reagent sufficiently clears tissue for 3D fluorescent imaging, but unlike other clearing techniques does not significantly change the tissue morphology. Minimal morphological changes, such as shrinkage and contraction, have been observed. For precious samples, the clearing can be reversed, and the tissue can be processed for histology applications such as hematoxylin and eosin (H&E) staining.

The Invitrogen PureLink RNA Micro Scale Kit is designed to be used when input is limited. It provides rapid purification and concentration of total RNA from a variety of samples, including LCM tissues, fine-needle aspirates, and sorted cells.

Unlocking the information contained in FFPE samples is essential to cancer research. The Applied Biosystems MagMAX FFPE DNA/RNA Ultra Kit is designed for sequential isolation of DNA and RNA from the same FFPE tissue sample, with a minimal requirement of a 5 μm section or curl. The DNA and RNA are recovered in separate eluates, and both are compatible with a broad range of applications, including real-time PCR and NGS.

Invitrogen SYBR Gold Nucleic Acid Gel Stain is the most sensitive fluorescent stain we offer for nucleic acid detection. Maximize your precious DNA and RNA samples with enhanced sensitivity to detect as little as 25 pg of sample.

Invitrogen Platinum SuperFi DNA Polymerase is a proofreading enzyme that combines superior fidelity with trusted Platinum hot-start technology. Featuring >100x the fidelity of Taq polymerase, Platinum SuperFi DNA Polymerase is ideally suited for applications that benefit from exceptional sequence accuracy. In addition, the extreme robustness of the polymerase enables successful amplification of samples of suboptimal purity.

With simple cartridge-loaded reagents and a straight-forward user interface, the Ion GeneStudio S5 System makes NGS fast and easy—ideal for any cancer research lab. Minimize sample input and maximize flexibility. Ion AmpliSeq technology enables successful library preparation from challenging sample types such as formalin-fixed, paraffin-embedded (FFPE) tissue, retrospective samples from fine-needle aspirates, and cell-free DNA (cfDNA) extracted from blood, using as little as 1 ng of input DNA or RNA to support multiple applications. With this system, you can efficiently implement small and large projects across multiple research applications with a simple sample-to-data NGS workflow.

Applied Biosystems TaqMan Advanced miRNA assays enable highly sensitive and specific quantitation of mature miRNAs using qPCR. Together with the TaqMan Advanced miRNA cDNA Synthesis Kit, this solution provides a streamlined workflow that allows for the detection and quantitation of mature miRNA from as little as 1 pg of total RNA or 2 μL of plasma or serum.

Invitrogen SuperScript IV Reverse Transcriptase offers superior cDNA synthesis in a short 10-minute reaction. It is designed to provide reliable and consistent results even with the most challenging RNA samples.

The Ion Torrent Oncomine TCR Beta-LR Assay is designed to efficiently capture all three complementarity determining regions of the TCR beta chain gene (CDR1, CDR2, CDR3) with as little as 10ng of RNA input. This assay enables key applications such as predictive or prognostic biomarker discovery, T cell characterization, and identification of variable gene polymorphisms from samples such as RNA extracted from whole blood, fresh-frozen tissue, or sorted cells. The use of RNA template allows sequencing of productive and relevant variable (V), diversity (D), and joining (J) rearrangements—improving the identification of rare clones.

With a 3-day workflow using as little as 20 ng of tumor DNA, the Ion Torrent Oncomine Tumor Mutation Load Assay covers 1.65 Mb across 409 oncogenes relevant to major cancer types. The assay highly correlates with exome mutation counts and obviates the need for whole-exome sequencing, allowing for a higher percentage of samples to be evaluated and conserving precious samples for additional biomarker assessment. Additionally, our Oncomine informatics enable simultaneous assessment of TMB and variant profiling for greater actionable insights.

The Applied Biosystems Arcturus Cellect LCM System is optimized for applications ranging from single-cell isolation to extraction of small cell populations with a high degree of precision and flexibility. The instrument enables researchers to uncover unique molecular signatures that may be obscured in heterogeneous tumor samples. This system features the unique combination of a gentle infrared (IR) laser to capture the cells of interest and a powerful ultraviolet (UV) laser for efficient microdissection.

Applied Biosystems TaqMan Mutation Detection assays are powered by allele-specific TaqMan PCR (castPCR) technology to detect and measure somatic mutations in genes of interest to cancer research. This highly specific and sensitive technology can detect rare copies of mutated DNA in a sample that contains large amounts of wild type DNA—down to 1 cancer cell in 1,000 normal cells. These assays are compatible with sample types such as cell lines, FFPE tissue, and fresh-frozen tissue.

The Invitrogen Qubit Fluorometer is designed to quickly and specifically quantitate DNA or RNA. In addition, with the newest version of the Qubit Fluorometer, you can now easily measure RNA integrity and quality. It is now easier than ever to make sure you have enough DNA or RNA for your experiment. Use as little as 1 μL of sample, even with very dilute samples. Dyes in Invitrogen Qubit assays bind selectively to DNA, RNA, or protein, making them more sensitive than UV absorbance.

When working with challenging RNA samples or analyzing low-abundance transcripts, the ability of a reverse transcriptase to generate cDNA from the lowest amount of input RNA is crucial. Invitrogen SuperScript IV VILO Master Mix offers highly sensitive and robust cDNA synthesis for RT-qPCR applications in a short 10-minute reaction. Obtain higher cDNA yields and earlier Ct values (up to 6 cycles ahead of other reverse transcriptases), all while maintaining superior linearity across a broad range of RNA inputs. Additionally, superior inhibitor resistance allows for the utmost confidence in results, even with degraded or inhibitor-containing RNA samples.

Requiring low sample input, the Ion Torrent Oncomine TCR Beta-SR Assay specifically interrogates the CDR3 region of the TCR beta chain gene. Compatible with both FFPE DNA and RNA, this short-read sequencing assay enables characterization of immune status and detection of T cell clones in peripheral blood. The assay offers a 2-day turnaround time and is complete with superior informatics for accurate clonality and sequence assessment of the CDR3 region without interference from primer bias.

Clariom D Pico and Clariom S Pico microarray assays

Many expression profiling techniques require large amounts of input RNA, which are often not available in cancer research samples. Applied Biosystems Clariom Pico assays can generate detailed, robust, and accurate data from as little as 100 pg of total RNA (as few as 10 cells) from a wide range of challenging clinical research samples.

Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is an ultrasensitive enhanced chemiluminescent (ECL) substrate for low-femtogram detection of protein by western blot analysis with the enzyme horseradish peroxidase (HRP). This substrate produces a quantitative signal that is easily captured by exposure to film or with an imaging system. The substrate offers excellent signal duration—8 hours of usable light output when conditions are optimized.

Invitrogen Bolt Bis-Tris Plus mini gels are designed for optimal separation of a broad molecular weight range of proteins under denaturing conditions during gel electrophoresis. The unique WedgeWell design allows loading of up to two times more sample volume than other precast gels. Bolt gels are ideal for western blot analysis and applications where protein integrity is crucial and higher sensitivity is required.