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The latest version of our TaqMan Assay search tool allows you to search more than 20 million professionally designed assay and array products backed by the TaqMan performance guarantee. The new features of the tool include the latest and most relevant publications associated with each assay. Below you will find a quick overview of how to navigate the search tool and find the right solution for your research. TaqMan Assays are consistently used as a trusted source, generating over 40,000 publications—3X more than any other 5’ nuclease assay.
The TaqMan Assay search tool includes assays and arrays for the following applications:
4. If desired, refine your search with filters (species, assay attributes, and more) (Figure 2).
If you are looking for multiple assays for a project or are creating a list of assays to include on an array, you can search multiple targets at once:
For gene expression assays and microRNA assays, you can enter a target sequence to find predesigned assays that map to that sequence:
For SNP genotyping and copy number assays, you can enter a chromosome position to find assays that map to that location:
When searching for TaqMan Arrays, you can search using any of the following types of keywords:
To order a TaqMan Assay, two pieces of information are required:
The catalog number for a TaqMan Assay is sequence-agnostic and defines other attributes of the assay, including:
The assay ID is a unique identifier for each assay designed either as part of Thermo Fisher Scientific’s comprehensive collection of pre-designed assays or as a custom assay using one of our custom assay design tools.
Use the TaqMan Assay search tool to search for an assay (see "How do I search for TaqMan assays and arrays?").
In the search result, the assay ID is highlighted in bold at the top of the assay result box. The catalog number is listed directly below and will change if you make changes to the dye or size (Figure 1).
There are multiple ways to reorder an assay:
We offer many tools to help you decide which gene expression assay is right for you:
All TaqMan Assays are designed using our industry-leading bioinformatics design process and meet all of our high quality-control specifications. For any given target, we compare all assays for that target and select a Best Coverage assay for standard gene expression experiments that detects the maximum number of transcripts for the gene of interest. We also evaluate the assays based on the design criteria below with the recommended assay best meeting the criteria.
For any gene expression assay, information about the assay design and the transcripts it detects is provided.
In the assay result box, Assay Design indicates where the primers and probe are located within the exon structure of the transcript and is updated regularly. Clicking the link under Assay Design opens the section under "View Details", revealing more details about the Assay Design (Figure 2).
Clicking the link under Transcripts also opens the View Details section and shows RefSeq accession information for the transcripts detected by the assay.
You can view the number of publications that cite the assay and a list of those publications in the assay search results (Figure 3). (For more information, see "How do I see publications for an assay?"). The assay result with the most citations is also marked with a banner.
Important Information text boxes provide information that you may want to consider in determining which assay is best for your experiment (Figure 4). Types of information found in these text boxes include:
Filters are always available at the top of the search page to help you refine your search results. (If you do not see them, click View filters to the right of the search bar). For gene expression results, there are multiple parameters to filter on, including Cross Reactivity.
The Cross Reactivity filter is used to check assays for cross-species reactivity for use in transgenic animal experiments. When you select a species in the Cross Reactivity filter, the list of results will be updated such that any assays that may cross react with the species selected will be excluded from the results. Any assays that remain in the list will not exhibit species cross reactivity and thus are suitable to use in a transgenic study.
You can now see the latest publications for an assay directly in the search results. For each assay ID, you can find the following publication-related information:
You can export search results by selecting the assays or arrays to be included using the checkboxes (Figure 1) and then opening the Select action dropdown.
In the Select action dropdown, there are two options for exporting your assay search results:
The exported files contain detailed information about the assays you have selected. For example, gene expression assay export files will include the following information for each assay ID selected:
If you are exporting information for TaqMan Array products, you will get an xls file with a tab for each product you selected (identified by catalog number or array ID) and the assay layout information for each array.
For Research Use Only. Not for use in diagnostic procedures.