Mutation Detection Analysis Using TaqMan Assays | Thermo Fisher Scientific

TaqMan™ Mutation Detection Assays are powered by competitive allele-specific TaqMan™ PCR (castPCR™ Technology) to detect and measure somatic mutations in genes associated with cancer research. The castPCR technology is highly specific and sensitive, and can detect rare amounts of mutated DNA in a sample that contains large amounts of normal, wild-type DNA. These assays are compatible with different sample types, such as cell lines, formalin-fixed paraffin-embedded (FFPE) tissue samples, and fresh frozen tissue samples.

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castPCR technology

Competitive, allele-specific TaqMan PCR utilizes an allele-specific primer for mutant allele detection that competes with an MGB blocker oligonucleotide to suppress the wild-type background.

Somatic mutation detection

By combining the power of TaqMan and castPCR technologies, TaqMan Mutation Detection Assays give you:

Higher specificity—designed to block the wild-type and amplify only the mutant
Higher sensitivity—designed to detect somatic mutations down to 1 cancer cell in 1,000 normal cells
Faster workflow—3 hours from sample to result

With 819 assays for 47 known cancer research genes available today like KRAS, BRAF, KIT, JAK2, the TaqMan Mutation Detection Assays provide an innovative tool for your cancer research.

Learn more about the somatic mutation detection workflow

All formats and custom designed assays

TaqMan Mutation Detection IPC Reagent Kit

The TaqMan™ Mutation Detection IPC Reagent Kit is a set of optional internal positive control reagents that can be duplexed with any TaqMan Mutation Detection Assay to provide a positive PCR control result. The TaqMan Mutation Detection IPC Reagent Kit is pre-optimized for use with the TaqMan Mutation Detection Assays.

96- or 384-well plates

Order your TaqMan Mutation Detection Assays pre-plated into Fast or standard 96- or 384-well plates with the TaqMan™ Custom Plating Service. Save time and reduce the resources needed for large-scale experiments.

Data analysis software

For data analysis, Applied Biosystems™ Mutation Detector™ Software allows users to determine the mutation status of their samples from TaqMan Mutation Detection Assay data collected on Applied Biosystems™ Real-Time PCR Systems.

TaqMan Assay documentation files

The TaqMan documentation files can be downloaded at thermofisher.com/taqmanfiles

Discrimination	castPCR technology combines allele-specific TaqMan qPCR with allele-specific MGB oligonucleotide blocker to effectively suppress non-specific amplification from the off-target allele.
Assay Components	Each pre-formulated mutant or wild type allele assay contains: A 10X mix of unlabeled PCR primers, a TaqMan™ MGB probe (FAM™ dye-labeled), and an MGB oligonucleotide blocker Each pre-formulated gene reference assay contains: A 10X mix of unlabeled PCR primers and a TaqMan MGB probe (FAM™ dye-labeled)
Reactions	Sufficient for 150 10µl reactions in a 384-well plate or 75 20µl reactions in a 96-well plate.
Expiration Date	2 years from the date of manufacture
Assay Information File	Includes sales order number, part number, assay ID, NCBI gene name, and entrez gene ID. Also includes Sanger COSMIC ID, nucleotide mutation, amino acid change, and mutation type.
Documentation	Available to download at thermofisher.com/taqmanfiles: Protocol for TaqMan Mutation Detection Assays (pdf file) TaqMan Mutation Detection Assays Quick Reference Card (pdf file) Product Insert (pdf file) Assay Information File (XML and HTML formats) Understanding Your shipment document (pdf file) Safety data sheet (pdf file) Datasheet (pdf file) Certificate of Analysis (pdf file)
Tracking/Identification	2D barcode laser-etched on the bottom of each assay tube 1D barcode printed on each rack of tubes
Shipping and Storage	Shipped at ambient temperature. Store at –15ºC to –25ºC. White Paper: Ambient Temperature Shipping Reduces Environmental Impact and Retains Quality and Stability

Catalog #	Product Name	Availability
4465804	TaqMan Mutation Detection Assays (mutant allele assays and wild type allele assays)	Inventoried
4465805	TaqMan™ EGFR Exon 19 Deletions Assay (mutant allele assay to 19 different EGFR exon 19 deletions)	Inventoried
4465807	TaqMan Mutation Detection Reference Assays (gene reference assays)	Inventoried
4467538	TaqMan Mutation Detection IPC Reagent Kit	Inventoried

Documentation

TaqMan Assay documentation files

The TaqMan documentation files can be downloaded at thermofisher.com/taqmanfiles

Manuals & Protocols

Product Literature

Product Bulletin: TaqMan Mutation Detection Assays
Application Note: Accurate and sensitive mutation detection and quantitation
Poster: Cancer Biomarker Research Using castPCR Technology

Related applications

Mutation Detection With Real-Time PCR

Data Analysis Tools

Mutation Detector™ Software

Specifications

Discrimination	castPCR technology combines allele-specific TaqMan qPCR with allele-specific MGB oligonucleotide blocker to effectively suppress non-specific amplification from the off-target allele.
Assay Components	Each pre-formulated mutant or wild type allele assay contains: A 10X mix of unlabeled PCR primers, a TaqMan™ MGB probe (FAM™ dye-labeled), and an MGB oligonucleotide blocker Each pre-formulated gene reference assay contains: A 10X mix of unlabeled PCR primers and a TaqMan MGB probe (FAM™ dye-labeled)
Reactions	Sufficient for 150 10µl reactions in a 384-well plate or 75 20µl reactions in a 96-well plate.
Expiration Date	2 years from the date of manufacture
Assay Information File	Includes sales order number, part number, assay ID, NCBI gene name, and entrez gene ID. Also includes Sanger COSMIC ID, nucleotide mutation, amino acid change, and mutation type.
Documentation	Available to download at thermofisher.com/taqmanfiles: Protocol for TaqMan Mutation Detection Assays (pdf file) TaqMan Mutation Detection Assays Quick Reference Card (pdf file) Product Insert (pdf file) Assay Information File (XML and HTML formats) Understanding Your shipment document (pdf file) Safety data sheet (pdf file) Datasheet (pdf file) Certificate of Analysis (pdf file)
Tracking/Identification	2D barcode laser-etched on the bottom of each assay tube 1D barcode printed on each rack of tubes
Shipping and Storage	Shipped at ambient temperature. Store at –15ºC to –25ºC. White Paper: Ambient Temperature Shipping Reduces Environmental Impact and Retains Quality and Stability

View all sizes

Catalog #	Product Name	Availability
4465804	TaqMan Mutation Detection Assays (mutant allele assays and wild type allele assays)	Inventoried
4465805	TaqMan™ EGFR Exon 19 Deletions Assay (mutant allele assay to 19 different EGFR exon 19 deletions)	Inventoried
4465807	TaqMan Mutation Detection Reference Assays (gene reference assays)	Inventoried
4467538	TaqMan Mutation Detection IPC Reagent Kit	Inventoried

Mutation analysis using digital PCR

The power of digital PCR can also be used to detect rare mutations for cancer research. Using the QuantStudio™ 3D Digital PCR System and wet-lab validated TaqMan SNP Genotyping Assays, you can target over 100 somatic mutations at a prevalence as low as 0.1%.

Learn more about digital PCR for rare mutation analysis

Resources