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Prohibited Substance Screening
Meet current and future guidelines
Whether you are accredited to the World Anti-Doping Agency (WADA) or one of many other global and regional governing bodies, keeping up to date on current prohibited substance screening isn’t easy.
Thermo Fisher Scientific is routinely selected to support in and out of competition testing with high-throughput instruments for screening large sets of compounds efficiently in a single methods approach, while staying ahead of future banned substances.
Featured prohibited list screening products
Featured categories
Answer your most challenging sport testing applications with our high-performance GC-MS & GC-MS/MS systems.
To ensure athletes play true, our market leading solutions help you profile athletes and confirm steroid use with the precision and sensitivity expected.
Whether you require high-throughput, highly sensitive quantitative performance or screening solutions to retrospectively answer tomorrow's doping questions, we have you covered.
Sample preparation is a key step in analytical workflow. Our solutions include a multitude of consumables and automated platforms to enable fast, reproducible and easy sample preparation—whatever your workflow requirements may be.
Confirm the presence of synthetic forms of endogenous anabolic androgenic steroids (EAAS) in urine samples.
Comprehensive screening of known and unknown performance enhancing substances is a critical part of maintaining sports true.
Typical prohibited substance screening workflows
Solve your most challenging applications with our high-performance GC-MS and LC-MS systems.
Sample preparation
Prohibited substance testing routinely screens athletes for hundreds of compounds in a number of WADA Classes. Not all hyphenated mass spectrometry workflows offer the sensitivity or performance capabilities necessary to measure the analytes of interest, laboratories utilize a variety of configurations. Starting with typically urine-based samples, laboratories will need to utilize a multi-step preparation to maximize system sensitivity.
GC-MS workflows (non-peptidic):
Hydrolysis + Extraction + Derivatization
LC-MS workflows (peptidic & non-peptidic):
Hydrolysis + Extraction
Sample preparation application handbook
Access the handbook to learn more about the variety of forensic toxicology and sport doping sample preparation options available to you.
Screen and qualify
Sports testing laboratories rely on a variety of mass spectrometry based workflows to test for various WADA Classes of prohibited substances. Below is a comparative table of the various techniques. As technologies advance with increased performance, we continue to see a shift in platforms used.
Which mass spectrometer is right for you?
 TRACE 1610 Gas Chromatograph and ISQ 7610 Single Quadrupole GC-MS System |  TRACE 1610 Gas Chromatograph and TSQ 9610 Triple Quadrupole GC-MS/MS System |  UltiMate 3000 RS HPLC systems and TSQ Altis Triple Quadrupole mass spectrometer |  UltiMate 3000 RSLCnano system and Q Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer | |
| Targeted Screening |  | | | |
| Quantitation | | | | |
| Unknown Profiling & Retrospective Screening |  | | | |
| Typical WADA classes: S1, S3, S4, S5, S6, S7, S8 | | | | * |
| Typical WADA classes: SO, S2, S4.5, S9, P1, P2 | | | | * |
| Sample throughput | Low to medium | Low to medium | High | Medium to high |
| Cost | $ | $$ | $$$ | $$$ |
| Sample prep | Moderate | Moderate | Minimal | Minimal |
| Dynamic range | 7 logs | 7 logs | ≤ 6 logs | ≤ 6 logs |
* not all applications may require nanoHPLC
Analysis
TraceFinder software offers the most comprehensive and quantitative workflows to help you address critical challenges in your routine or advanced quantitation assays.
Sample preparation
Prohibited substance testing routinely screens athletes for hundreds of compounds in a number of WADA Classes. Not all hyphenated mass spectrometry workflows offer the sensitivity or performance capabilities necessary to measure the analytes of interest, laboratories utilize a variety of configurations. Starting with typically urine-based samples, laboratories will need to utilize a multi-step preparation to maximize system sensitivity.
GC-MS workflows (non-peptidic):
Hydrolysis + Extraction + Derivatization
LC-MS workflows (peptidic & non-peptidic):
Hydrolysis + Extraction
Sample preparation application handbook
Access the handbook to learn more about the variety of forensic toxicology and sport doping sample preparation options available to you.
Screen and qualify
Sports testing laboratories rely on a variety of mass spectrometry based workflows to test for various WADA Classes of prohibited substances. Below is a comparative table of the various techniques. As technologies advance with increased performance, we continue to see a shift in platforms used.
Which mass spectrometer is right for you?
| TRACE 1610 Gas Chromatograph and ISQ 7610 Single Quadrupole GC-MS System | TRACE 1610 Gas Chromatograph and TSQ 9610 Triple Quadrupole GC-MS/MS System | UltiMate 3000 RS HPLC systems and TSQ Altis Triple Quadrupole mass spectrometer | UltiMate 3000 RSLCnano system and Q Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer | |
| Targeted Screening | | | | |
| Quantitation | | | | |
| Unknown Profiling & Retrospective Screening | | | | |
| Typical WADA classes: S1, S3, S4, S5, S6, S7, S8 | | | | * |
| Typical WADA classes: SO, S2, S4.5, S9, P1, P2 | | | | * |
| Sample throughput | Low to medium | Low to medium | High | Medium to high |
| Cost | $ | $$ | $$$ | $$$ |
| Sample prep | Moderate | Moderate | Minimal | Minimal |
| Dynamic range | 7 logs | 7 logs | ≤ 6 logs | ≤ 6 logs |
* not all applications may require nanoHPLC
Analysis
TraceFinder software offers the most comprehensive and quantitative workflows to help you address critical challenges in your routine or advanced quantitation assays.
Clinical Research and Forensics Handbook
In our latest application handbook, we provide access to sports antidoping and forensic drug chemistry applications, peer reviewed references and recent technical posters for a wide range of experimental techniques, including mass spectrometry (including single quadrupole, triple quadrupole and quantitative high resolution systems), liquid chromatography and a variety of sample prep solutions.
Featured webinar
In this webinar Dr. Alessandro Casilli, Brazilian Laboratory for Doping Control, recent advances in high resolution accurate mass (HRAM-MS) Orbitrap technology for measuring different ionization modes during the same run, and improving specificity and detection limits in sports doping control analyses.
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