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The Varioskan® Flash from Thermo Scientific has received the LanthaScreen® Certified designation from Life Technologies which ensures that this reader is validated to strict standards in instrument setup and assay performance.
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Before using LanthaScreen® Terbium and Europium assays
Test your plate reader set-up
These technical notes provide a method for verifying that a fluorescent plate reader is able to detect a change in time-resolved fluorescence energy transfer (TR-FRET) signal, confirming proper instrument set-up and a suitable response. The method is independent of any biological reaction or equilibrium and uses reagents that are on-hand for the LanthaScreen® assay.
Varioskan® Flash Multimode Reader
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Instrument Configuration:
| Assay Technology | Adapta® & LanthaScreen® Europium | GeneBLAzer® | LanthaScreen® Terbium | Predictor™ | Z'-LYTE® |
|---|---|---|---|---|---|
| Modality | TR-FRET | FRET (bottom) | TR-FRET | FP | FRET |
| Setup Guide | Reader not |
| Reader not | Reader not compatible |
|
| Application Note | - | - | - | - | - |
| Poster | - | - | - | - | - |
| Excitation | 340/12 | 409/12 | 332/12 | - | 400/12 |
| Emission 1 | 615/12 | 460/12 | 488/12 | - | 460/12 |
| Emission 2 | 665/12 | 530/12 | 518/12 | - | 530/12 |
* Optics shown as center wavelength/bandwidth (nm); Contact Thermo Scientific for information on detection modalities suitable with the microplate reader listed above.
Varioskan® is a registered trademark of Thermo Scientific.
For Research Use Only. Not for use in diagnostic procedures.