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Our LanthaScreen Nuclear Receptor Competitive Binding Assay contains all the needed reagents you need to conduct compound affinity testing:
The nuclear receptor (NR) protein is indirectly labeled with terbium via the anti-GST antibody bound to the GST tag on the receptor. When this labeled protein binds to the Fluormone™ ligand, a high TR-FRET ratio will result. If a test compound that binds to the NR protein is added to the reaction, the test compound will compete with the Fluormone™ ligand, resulting in a lower TR-FRET ratio. Since the change in TR-FRET ratio only occurs in the presence of a competitor, it can be used as a convenient and accurate indicator of the relative affinity of the test compound for the NR (Figure 1 below).
Figure 1. Assay schematic of a Nuclear Receptor LanthaScreen®
Target | Alias(es) | Accession number | LanthaScreen® Competitive Binding Assay (TR-FRET) |
---|---|---|---|
Estrogen receptor alpha | ER Alpha, ERA, ESR1, NR3A1 | NP_000116.2 | A15887 |
Estrogen receptor beta | ER Beta, ERB, ESR2, NR3A2 | NP_001428.1 | A15895 |
Glucocorticoid receptor | GR, GCCR, GCR, NR3C1 | NP_000167 | A15901 |
Peroxisome proliferator-activated receptor alpha | PPAR Alpha, PPARA, NR1C1 | NP_005027 | PV4892 |
Peroxisome proliferator-activated receptor delta | PPAR Delta, PPARD, NR1C2 | NP_006229 | PV4893 |
Peroxisome proliferator-activated receptor gamma | PPAR Gamma, PPARG, NR1C3 | NP_005028 | PV4894 |
Pregnane X receptor | PXR(SXR), NR1I2 | NP_003880.3 | PV4839 |
For Research Use Only. Not for use in diagnostic procedures.