Targeted Genotyping by Sequencing | Thermo Fisher Scientific

Targeted genotyping by sequencing (GBS) is the latest innovation in genomics-assisted breeding programs. Targeted GBS uses a powerful multiplexed approach that enables users to interrogate hundreds to thousands of markers across hundreds to thousands of samples simultaneously. It includes NGS-based assays designed using customer-provided markers or catalog panels designed with publicly available markers.

Applications

Marker-assisted selection and breeding
Parentage and traceability

Benefits

Fast turnaround time
Cost effective
Simplified data analysis

Thermo Fisher Scientific offers two targeted genotyping by sequencing solutions:

Applied Biosystems AgriSeq targeted GBS solutions

The table below highlights the key features of our Applied Biosystems AgriSeq and targeted GBS technology.

	AgriSeq targeted GBS solutions
Requires Ion Torrent instrument	Yes
SNPs per panel	50–5,000
Ability to discover new variants	Yes
Expected call rates*	–95%
Processing time	2 days
Panel design	2–6 weeks for custom panel design
Sample throughput/week	Up to 7,680**
Cost per sample	Low

* Depends on level of genome annotation. ** Based on panel size with one instrument and one technician.