Antibody Purification Using Protein A and Alkaline Stable Protein A

Antibody purification involves isolation of antibody from serum (polyclonal antibody), ascites fluid, or from the culture supernatant of a hybridoma cell line (monoclonal antibody). Protein A ligand is recommended specifically for polyclonal IgG from rabbit, pig, dog, or cat serum. It binds the heavy chain constant region (Fc) of IgG (CH2-CH3 region). Native Protein A is purified from Staphylococcus aureus (46.7 kDa; 4 IgG-binding sites). The POROS MabCapture A resins feature a recombinant Protein A (rPA) ligand produced in yeast that can be scaled up for bioprocess applications.

The MabCaptureC™ High Capacity Protein A is a high-performance chromatography resin for capturing mAbs and recombinant Fc-tagged fusion proteins. It provides high specific binding capacity and enhanced alkaline stability characteristics under clean-in-place conditions (CIP), for a more robust purification and resin regeneration.

We offer a wide selection of base supports and formats to purify antibodies using the Protein A ligand. Our portfolio is designed to meet small-scale (screening) up to large-scale (bioprocess) needs.

Multiple formats—magnetic beads, loose resin, spin columns and kits, FPLC cartridges, and 96-well filter plates enable antibody purification from microgram to kilogram scales
Alkaline stability—engineered to withstand common CIP procedures with 0.2 M NaOH enabling efficient cleaning of the chromatography resin
High performance—resins are designed to maximize protein yield and reduce background
Economical—pricing is similar to or better than our leading competitors

Choose the right Protein A product for antibody purification

Product links in the last row of each column

	Dynabeads Protein A Magnetic Beads	Pierce Protein A Agarose	Pierce Protein A Plus Agarose	Pierce Recombinant Protein A Agarose	MabCaptureC™ High Capacity Protein A, Alkaline-Stable
Bead or resin size	2.8 μm	45–165 μm	45–165 μm	45–165 μm	75 μm
Binding capacity	250–300 μg human IgG/mL beads	12–19 mg human IgG/mL resin	34 mg human IgG/mL resin	15–17 mg human IgG/mL resin	58 mg/ml at 5 min residence (80 mg/ml at 10 min residence) – 10% breakthrough
Maximum linear flow rate	N/A	700 cm/hr	700 cm/hr	700 cm/hr	300 cm/hr or less (150 cm/hr recommended)
Support	Monosized, super paramagnetic polystyrene beads	Crosslinked 6% beaded agarose	Crosslinked 6% beaded agarose	Crosslinked 6% beaded agarose	Highly cross-linked agarose
Recommended application scale	screening	batch, pilot	screening, batch	batch	batch, pilot, process
Formats available	loose beads	loose beads, chromatography cartridges	loose beads, pre-packed spin columns and kits, 96-well filter plates	loose beads	loose beads
Order product(s)	10001D	20333 (loose resin) 88924 (1 mL cartridge) 89925 (5 mL cartridge) 44667 (spin kit)	22810 (loose resin) 45202 (spin plates)	20365	A53031, 10 mL 50% slurry A53033, 50 mL 50% slurry A53034, 200 mL 50% slurry 1963662250, 250 mL 50% slurry 196366201L, 1 L 50% slurry 196366205L, 5 L 50% slurry 196366210L, 10 L 50% slurry

For Research Use Only. Not for use in diagnostic procedures.