Alkaline Phosphatase Conjugated Secondary Antibodies

AP conjugated mouse IgG (H+L) cross-adsorbed secondary antibody in western blotting

Alkaline phosphatase (AP)-conjugated secondary antibodies are commonly used in enzyme driven applications, such as western blotting, ELISA, immuno-histochemistry, and others. AP-conjugated secondary antibodies use alkaline phosphatase substrates mainly for chemiluminescent or colorimetric detection. Navigate this page to find our menu of AP-conjugated secondary antibodies along with recommended substrates for your western blotting, ELISA, or immuno-histochemistry experiments.

Search all AP-Conjugated Secondary Antibodies

AP-conjugated secondary antibodies by application

Our offerings for high-activity AP-conjugated secondary antibodies are sorted below in the tabular format by application. Included with the selection tables for AP-linked secondary antibodies are recommended substrates for each application by detection method, including chemiluminescent or colorimetric.

Table 1. Alkaline phosphatase secondary antibodies for western blotting

Target species	Host
Target species	Goat	Rabbit	Mouse	Donkey	Chicken	Sheep
Anti-Mouse	AP-conjugated goat anti-mouse	AP-conjugated rabbit anti-mouse	—	AP-conjugated donkey anti-mouse	—	—
Anti-Human	AP-conjugated goat anti-human	AP-conjugated rabbit anti-human	AP-conjugated mouse anti-human	—	—	AP-conjugated sheep anti-human
Anti-Rabbit	AP-conjugated goat anti-rabbit	—	—	AP-conjugated donkey anti-rabbit	AP-conjugated chicken anti-rabbit	—
Anti-Rat	AP-conjugated goat anti-rat	AP-conjugated rabbit anti-rat	—	AP-conjugated donkey anti-rat	AP-conjugated chicken anti-rat	AP-conjugated sheep anti-rat
Anti-Goat	—	AP-conjugated rabbit anti-goat	—	AP-conjugated donkey anti-goat	AP-conjugated chicken anti-goat	—
Anti-Bovine	AP-conjugated goat anti-bovine	AP-conjugated rabbit anti-bovine	—	—	—	—
Anti-Chicken	AP-conjugated goat anti-chicken	AP-conjugated rabbit anti-chicken	—	—	—	—
Anti-Hamster	AP-conjugated goat anti-hamster	AP-conjugated rabbit anti-hamster	—	—	—	—
Anti-Guinea Pig	AP-conjugated goat anti-guinea pig	AP-conjugated rabbit anti-guinea pig	—	—	—	—
Anti-Sheep	—	AP-conjugated rabbit anti-sheep	—	AP-conjugated donkey anti-sheep	—	—
Anti-Dog	AP-conjugated goat anti-dog	—	—	—	—	—
Anti-Camelid	AP-conjugated goat anti-llama	AP-conjugated rabbit anti-llama	—	—	—	—
Anti-Donkey	AP-conjugated goat anti-donkey	AP-conjugated rabbit anti-donkey	—	—	—	—

Recommend AP substrates for western blotting

1-Step™ NBT/BCIP Substrate Solution	Novex AP Chemiluminescent Substrate (CDP-Star)

Readout: Colorimetric	Readout: Chemiluminescent
Low background, ready-to-use format	High sensitivity, ready-to-use format
Recommended antibody dilution: 1°: 1:500 2°: 1:5,000 to 1:50,000	Recommended antibody dilution: 1°: 1:500 to 5,000 2°: 1:2,000 to 1:10,000
Order here	Order here

Table 2. Alkaline phosphatase secondary antibodies for ELISA applications

Target species	Host
Target species	Goat	Rabbit	Mouse	Donkey	Chicken	Sheep
Anti-Mouse	AP-conjugated goat anti-mouse	AP-conjugated rabbit anti-mouse	—	AP-conjugated donkey anti-mouse	—	—
Anti-Human	AP-conjugated goat anti-human	AP-conjugated rabbit anti-human	AP-conjugated mouse anti-human	—	—	AP-conjugated sheep anti-human
Anti-Rabbit	AP-conjugated goat anti-rabbit	—	—	AP-conjugated donkey anti-rabbit	AP-conjugated chicken anti-rabbit	—
Anti-Rat	AP-conjugated goat anti-rat	AP-conjugated rabbit anti-rat	—	AP-conjugated donkey anti-rat	AP-conjugated chicken anti-rat	AP-conjugated sheep anti-rat
Anti-Goat	—	AP-conjugated rabbit anti-goat	—	AP-conjugated donkey anti-goat	AP-conjugated chicken anti-goat	—
Anti-Bovine	AP-conjugated goat anti-bovine	AP-conjugated rabbit anti-bovine	—	—	—	—
Anti-Chicken	AP-conjugated goat anti-chicken	AP-conjugated rabbit anti-chicken	—	—	—	—
Anti-Hamster	AP-conjugated goat anti-hamster	AP-conjugated rabbit anti-hamster	—	—	—	—
Anti-Guinea Pig	AP-conjugated goat anti-guinea pig	AP-conjugated rabbit anti-guinea pig	—	—	—	—
Anti-Sheep	—	—	—	AP-conjugated donkey anti-sheep	—	—
Anti-Dog	AP-conjugated goat anti-dog	—	—	—	—	—
Anti-Camelid	AP-conjugate goat anti-llama	AP-conjugated rabbit anti-llama	—	—	—	—
Anti-Donkey	AP-conjugated goat anti-donkey	AP-conjugated rabbit anti-donkey	—	—	—	—

Recommend AP substrates for ELISA

1-Step PNPP Substrate Solution	CDP-Star Substrate (0.25 mM Ready-To-Use)

Readout: Colorimetric	Readout: Chemiluminescent
Order here	Order here

Table 3. Alkaline phosphatase secondary antibodies for IHC

Target species	Host
Target species	Goat	Rabbit	Mouse	Donkey	Chicken	Sheep
Anti-Mouse	AP-conjugated goat anti-mouse	AP-conjugated rabbit anti-mouse	—	AP-conjugated donkey anti-mouse	—	—
Anti-Human	AP-conjugated goat anti-human	AP-conjugated rabbit anti-human	AP-conjugated mouse anti-human	—	—	AP-conjugated sheep anti-human
Anti-Rabbit	AP-conjugated goat anti-rabbit	—	—	AP-conjugated donkey anti-rabbit	AP-conjugated chicken anti-rabbit	—
Anti-Rat	AP-conjugated goat anti-rat	AP-conjugated rabbit anti-rat	—	AP-conjugated donkey anti-rat	AP-conjugated chicken anti-rat	AP-conjugated sheep anti-rat
Anti-Goat	—	AP-conjugated rabbit anti-goat	—	AP-conjugated donkey anti-goat	AP-conjugated chicken anti-goat	—
Anti-Bovine	AP-conjugated goat anti-bovine	AP-conjugated rabbit anti-bovine	—	—	—	—
Anti-Chicken	AP-conjugated goat anti-chicken	—	—	—	—	—
Anti-Hamster	AP conjugated goat anti-hamster	AP conjugated rabbit anti-hamster	—	—	—	—
Anti-Guinea Pig	AP-conjugated goat anti-guinea pig	AP-conjugated rabbit anti-guinea pig	—	—	—	—
Anti-Sheep	—	—	—	AP-conjugated donkey anti-sheep	—	—
Anti-Dog	AP-conjugated goat anti-dog	—	—	—	—	—
Anti-Camelid	AP-conjugated goat anti-llama	AP-conjugated rabbit anti-llama	—	—	—	—
Anti-Donkey	AP-conjugated goat anti-donkey	AP-conjugated rabbit anti-donkey	—	—	—	—

Recommend AP substrates for IHC

1-Step™ NBT/BCIP Substrate Solution

Readout: Colorimetric
Low background, ready-to-use format
Recommended antibody dilution: 1°: 1:500 2°: 1:5,000 to 1:50,000
Order here

Western blotting

Table 1. Alkaline phosphatase secondary antibodies for western blotting

Target species	Host
Target species	Goat	Rabbit	Mouse	Donkey	Chicken	Sheep
Anti-Mouse	AP-conjugated goat anti-mouse	AP-conjugated rabbit anti-mouse	—	AP-conjugated donkey anti-mouse	—	—
Anti-Human	AP-conjugated goat anti-human	AP-conjugated rabbit anti-human	AP-conjugated mouse anti-human	—	—	AP-conjugated sheep anti-human
Anti-Rabbit	AP-conjugated goat anti-rabbit	—	—	AP-conjugated donkey anti-rabbit	AP-conjugated chicken anti-rabbit	—
Anti-Rat	AP-conjugated goat anti-rat	AP-conjugated rabbit anti-rat	—	AP-conjugated donkey anti-rat	AP-conjugated chicken anti-rat	AP-conjugated sheep anti-rat
Anti-Goat	—	AP-conjugated rabbit anti-goat	—	AP-conjugated donkey anti-goat	AP-conjugated chicken anti-goat	—
Anti-Bovine	AP-conjugated goat anti-bovine	AP-conjugated rabbit anti-bovine	—	—	—	—
Anti-Chicken	AP-conjugated goat anti-chicken	AP-conjugated rabbit anti-chicken	—	—	—	—
Anti-Hamster	AP-conjugated goat anti-hamster	AP-conjugated rabbit anti-hamster	—	—	—	—
Anti-Guinea Pig	AP-conjugated goat anti-guinea pig	AP-conjugated rabbit anti-guinea pig	—	—	—	—
Anti-Sheep	—	AP-conjugated rabbit anti-sheep	—	AP-conjugated donkey anti-sheep	—	—
Anti-Dog	AP-conjugated goat anti-dog	—	—	—	—	—
Anti-Camelid	AP-conjugated goat anti-llama	AP-conjugated rabbit anti-llama	—	—	—	—
Anti-Donkey	AP-conjugated goat anti-donkey	AP-conjugated rabbit anti-donkey	—	—	—	—

Recommend AP substrates for western blotting

1-Step™ NBT/BCIP Substrate Solution	Novex AP Chemiluminescent Substrate (CDP-Star)

Readout: Colorimetric	Readout: Chemiluminescent
Low background, ready-to-use format	High sensitivity, ready-to-use format
Recommended antibody dilution: 1°: 1:500 2°: 1:5,000 to 1:50,000	Recommended antibody dilution: 1°: 1:500 to 5,000 2°: 1:2,000 to 1:10,000
Order here	Order here

ELISA

Table 2. Alkaline phosphatase secondary antibodies for ELISA applications

Target species	Host
Target species	Goat	Rabbit	Mouse	Donkey	Chicken	Sheep
Anti-Mouse	AP-conjugated goat anti-mouse	AP-conjugated rabbit anti-mouse	—	AP-conjugated donkey anti-mouse	—	—
Anti-Human	AP-conjugated goat anti-human	AP-conjugated rabbit anti-human	AP-conjugated mouse anti-human	—	—	AP-conjugated sheep anti-human
Anti-Rabbit	AP-conjugated goat anti-rabbit	—	—	AP-conjugated donkey anti-rabbit	AP-conjugated chicken anti-rabbit	—
Anti-Rat	AP-conjugated goat anti-rat	AP-conjugated rabbit anti-rat	—	AP-conjugated donkey anti-rat	AP-conjugated chicken anti-rat	AP-conjugated sheep anti-rat
Anti-Goat	—	AP-conjugated rabbit anti-goat	—	AP-conjugated donkey anti-goat	AP-conjugated chicken anti-goat	—
Anti-Bovine	AP-conjugated goat anti-bovine	AP-conjugated rabbit anti-bovine	—	—	—	—
Anti-Chicken	AP-conjugated goat anti-chicken	AP-conjugated rabbit anti-chicken	—	—	—	—
Anti-Hamster	AP-conjugated goat anti-hamster	AP-conjugated rabbit anti-hamster	—	—	—	—
Anti-Guinea Pig	AP-conjugated goat anti-guinea pig	AP-conjugated rabbit anti-guinea pig	—	—	—	—
Anti-Sheep	—	—	—	AP-conjugated donkey anti-sheep	—	—
Anti-Dog	AP-conjugated goat anti-dog	—	—	—	—	—
Anti-Camelid	AP-conjugate goat anti-llama	AP-conjugated rabbit anti-llama	—	—	—	—
Anti-Donkey	AP-conjugated goat anti-donkey	AP-conjugated rabbit anti-donkey	—	—	—	—

Recommend AP substrates for ELISA

1-Step PNPP Substrate Solution	CDP-Star Substrate (0.25 mM Ready-To-Use)

Readout: Colorimetric	Readout: Chemiluminescent
Order here	Order here

Immunohistochemistry

Table 3. Alkaline phosphatase secondary antibodies for IHC

Target species	Host
Target species	Goat	Rabbit	Mouse	Donkey	Chicken	Sheep
Anti-Mouse	AP-conjugated goat anti-mouse	AP-conjugated rabbit anti-mouse	—	AP-conjugated donkey anti-mouse	—	—
Anti-Human	AP-conjugated goat anti-human	AP-conjugated rabbit anti-human	AP-conjugated mouse anti-human	—	—	AP-conjugated sheep anti-human
Anti-Rabbit	AP-conjugated goat anti-rabbit	—	—	AP-conjugated donkey anti-rabbit	AP-conjugated chicken anti-rabbit	—
Anti-Rat	AP-conjugated goat anti-rat	AP-conjugated rabbit anti-rat	—	AP-conjugated donkey anti-rat	AP-conjugated chicken anti-rat	AP-conjugated sheep anti-rat
Anti-Goat	—	AP-conjugated rabbit anti-goat	—	AP-conjugated donkey anti-goat	AP-conjugated chicken anti-goat	—
Anti-Bovine	AP-conjugated goat anti-bovine	AP-conjugated rabbit anti-bovine	—	—	—	—
Anti-Chicken	AP-conjugated goat anti-chicken	—	—	—	—	—
Anti-Hamster	AP conjugated goat anti-hamster	AP conjugated rabbit anti-hamster	—	—	—	—
Anti-Guinea Pig	AP-conjugated goat anti-guinea pig	AP-conjugated rabbit anti-guinea pig	—	—	—	—
Anti-Sheep	—	—	—	AP-conjugated donkey anti-sheep	—	—
Anti-Dog	AP-conjugated goat anti-dog	—	—	—	—	—
Anti-Camelid	AP-conjugated goat anti-llama	AP-conjugated rabbit anti-llama	—	—	—	—
Anti-Donkey	AP-conjugated goat anti-donkey	AP-conjugated rabbit anti-donkey	—	—	—	—

Recommend AP substrates for IHC

1-Step™ NBT/BCIP Substrate Solution

Readout: Colorimetric
Low background, ready-to-use format
Recommended antibody dilution: 1°: 1:500 2°: 1:5,000 to 1:50,000
Order here

Fluorescent substrates for signal amplification of AP conjugates

Figure 1. Endogenous phosphatase activity in zebra brain visualized with ELF Endogenous Phosphatase Kit
(Cat. No. E6601).

ELF signal amplification is based on ELF 97 phosphate, a substrate for both alkaline phosphatase and acid phosphatase. Upon cleavage this substrate yields a bright yellow-green fluorescence with excellent photostability. The ELF 97 signal is more photostable than signals achieved using either direct or indirect detection with fluorescein conjugates. ELF 97 is well suited for immunolabeling and can be detected with imaging instrumentation that is equipped with a UV light excitation source.

Order here

Do you need help deciding on which substrate to use? Please see our substrate selection tool
Need a primary antibody for a different application? Search for all your antibodies here
Have you seen our antibodies resource library? Find antibody methods, protocol, application notes, and more

For Research Use Only. Not for use in diagnostic procedures.