Fluorescence Basics | Thermo Fisher Scientific

Welcome to the Molecular Probes School of Fluorescence module on Fluorescence Basics. Here you will learn about photobleaching, fluorescence spectra, and filters and light sources.

Fluorescence Fundamentals

Introduction

Process of fluorescence
Photobleaching
The light spectrum and its relationship with fluorescence

Anatomy of Fluorescence Spectra

Spectra Basics

Excitation spectra
Emission spectra
Stokes Shift

Overview of Filters and Light Sources

Filters and light sources

Light sources
Lasers
LEDs
Excitation filters
Emission filters

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