Which Human CD4+ T Cell Isolation Product is Right for You?

  Highest purity for molecular assays Affordable & pure, flow compatible cells Extreme purity & physiological quality Untouched - most physiological relevant cells
Isolation MethodDepletion or positive isolationPositive isolationPositive isolationNegative isolation
Starting samplesAny sample (whole blood, MNC, bone marrow)Any sample (whole blood, MNC, bone marrow)Any sample (whole blood, MNC, bone marrow)MNC*
# MNC processed (mL whole blood processed)1-2 x 109 MNC
200 mL whole blood 		2 x 109 MNC
80 mL whole blood 		2 x 109 MNC
400 mL whole blood 		1 x 109 cells
PurityHighest
98-100% 		Higher
95-99% 		Highest
98-100% 		High
90-95%
YieldHighest
95-100% 		High
70-85% 		Standard
50-70% 		Standard
50-70%
Viability>95%>95%>95%>95%
Isolation Time~30 min~60 min~90 min~50 min
Isolation Temp.ColdColdRelease at room temp.Cold
ApplicationsDepletion of unwanted CD4+ T or positive isolation for molecular applications, e.g. isolation of proteins & nucleic acidsCellular assays (cell culture, cell activation/proliferation, cytokine studies)

Molecular isolation of proteins & nucleic acids 		Cellular assays (cell culture, cell activation/proliferation, cytokine studies)
Translational research

Molecular isolation of proteins & nucleic acids 		Cellular applications for physiological studies (functional studies, flow analysis, study of resting/activated cells)
Bead-free Target CellsNoYes (but antibody present)Yes (and antibody-free)Yes
Compatible with
Flow Cytometry
/Sorting 		NoYesYesYes
Shelf Life from Production Date3 years2 years3 years2 years
Required MaterialsMagnetsMagnetsMagnetsMagnets
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*MNC - Mononuclear cells

THIS IS FOR HUMAN RESEARCH - NOT FOR MOUSE
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