CellLight Fluorescent Protein Labeling

CellLight fluorescent proteins provide an easy way to label specific structures in live cells. Simply add the ready-to-use constructs to your cells, incubate overnight, and you’re ready to image in the morning. These constructs express fluorescent fusion proteins targeted to specific intracellular structures. The fluorescent protein is introduced using a simple transfection step, using the BacMam technology, that doesn’t require molecular biology techniques to carry out—it works like a cell stain.

See all CellLight fluorescent protein products

Using the BacMam 2.0-based CellLight fluorescent fusion proteins to express genes is as simple as it is efficient:

1. Add virus to cells in full medium.
2. Incubate overnight.
3. Image or freeze for future use.

Cells can be transduced by mixing cells with the CellLight fluorescent fusion protein immediately prior to plating. Alternatively, adherent cells can be transduced by addition of virus to cells already in dish or on plate. Typically, optimal transduction is obtained at 70% confluence and with a 20:1 BacMam particle to cell ratio.

When to use CellLight fluorescent fusion proteins:

• To target organelles or intracellular structures in live cells with minimal toxicity or chemical disruption
• To identify functions that require higher resolution
• To multiplex with other CellLight fluorescent proteins or organic dyes to follow the behavior of individual cells, and analyze in live cells or after fixation
• To label mitochondria irrespective of membrane potential and lysosomes irrespective of pH

When NOT to use CellLight fluorescent fusion proteins:

• During cell population studies or automated imaging/counting because CellLight fluorescent fusion proteins rarely result in 100% transfection rates (Transfection rates and conditions may differ by cell type as a result an extensive reference list on their uses is provided)